Use of the polymerase chain reaction for detection of Fusarium graminearum in bulgur wheat
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Food Science and Technology (Campinas) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612012000100030 |
Resumo: | The detection of mycotoxigenic fungi in foodstuff is important because their presence may indicate the possible associated mycotoxin contamination. Fusarium graminearum is a wheat pathogen and a producer of micotoxins. The polymerase chain reaction (PCR) has been employed for the specific identification of F. graminearum. However, this methodology has not been commonly used for detection of F. graminearum in food. Thus, the objective of the present study was to develop a molecular methodology to detect F. graminearum in commercial samples of bulgur wheat. Two methods were tested. In the first method, a sample of this cereal was contaminated with F. graminearum mycelia. The genomic DNA was extracted from this mixture and used in a F. graminearum specific PCR reaction. The F. graminearum species was detected only in samples that were heavily contaminated. In the second method, samples of bulgur wheat were inoculated on a solid medium, and isolates having F. graminearum culture characteristics were obtained. The DNA extracted from these isolates was tested in F. graminearum specific PCR reactions. An isolate obtained had its trichothecene genotype identified by PCR. The established methodology could be used in surveys of food contamination with F. graminearum. |
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Food Science and Technology (Campinas) |
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Use of the polymerase chain reaction for detection of Fusarium graminearum in bulgur wheatbulgur wheatFusarium graminearumPCRmolecular detectionfood protectionThe detection of mycotoxigenic fungi in foodstuff is important because their presence may indicate the possible associated mycotoxin contamination. Fusarium graminearum is a wheat pathogen and a producer of micotoxins. The polymerase chain reaction (PCR) has been employed for the specific identification of F. graminearum. However, this methodology has not been commonly used for detection of F. graminearum in food. Thus, the objective of the present study was to develop a molecular methodology to detect F. graminearum in commercial samples of bulgur wheat. Two methods were tested. In the first method, a sample of this cereal was contaminated with F. graminearum mycelia. The genomic DNA was extracted from this mixture and used in a F. graminearum specific PCR reaction. The F. graminearum species was detected only in samples that were heavily contaminated. In the second method, samples of bulgur wheat were inoculated on a solid medium, and isolates having F. graminearum culture characteristics were obtained. The DNA extracted from these isolates was tested in F. graminearum specific PCR reactions. An isolate obtained had its trichothecene genotype identified by PCR. The established methodology could be used in surveys of food contamination with F. graminearum.Sociedade Brasileira de Ciência e Tecnologia de Alimentos2012-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612012000100030Food Science and Technology v.32 n.1 2012reponame:Food Science and Technology (Campinas)instname:Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)instacron:SBCTA10.1590/S0101-20612012005000027info:eu-repo/semantics/openAccessFaria,Carla BertechiniAlmeida-Ferreira,Giovana CaputoGagliardi,Karina BertechineAlves,Tatiane Cristina AlbuquerqueTessmann,Dauri JoséMachinski Junior,MiguelBarbosa-Tessmann,Ione Parraeng2012-05-18T00:00:00Zoai:scielo:S0101-20612012000100030Revistahttp://www.scielo.br/ctaONGhttps://old.scielo.br/oai/scielo-oai.php||revista@sbcta.org.br1678-457X0101-2061opendoar:2012-05-18T00:00Food Science and Technology (Campinas) - Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)false |
dc.title.none.fl_str_mv |
Use of the polymerase chain reaction for detection of Fusarium graminearum in bulgur wheat |
title |
Use of the polymerase chain reaction for detection of Fusarium graminearum in bulgur wheat |
spellingShingle |
Use of the polymerase chain reaction for detection of Fusarium graminearum in bulgur wheat Faria,Carla Bertechini bulgur wheat Fusarium graminearum PCR molecular detection food protection |
title_short |
Use of the polymerase chain reaction for detection of Fusarium graminearum in bulgur wheat |
title_full |
Use of the polymerase chain reaction for detection of Fusarium graminearum in bulgur wheat |
title_fullStr |
Use of the polymerase chain reaction for detection of Fusarium graminearum in bulgur wheat |
title_full_unstemmed |
Use of the polymerase chain reaction for detection of Fusarium graminearum in bulgur wheat |
title_sort |
Use of the polymerase chain reaction for detection of Fusarium graminearum in bulgur wheat |
author |
Faria,Carla Bertechini |
author_facet |
Faria,Carla Bertechini Almeida-Ferreira,Giovana Caputo Gagliardi,Karina Bertechine Alves,Tatiane Cristina Albuquerque Tessmann,Dauri José Machinski Junior,Miguel Barbosa-Tessmann,Ione Parra |
author_role |
author |
author2 |
Almeida-Ferreira,Giovana Caputo Gagliardi,Karina Bertechine Alves,Tatiane Cristina Albuquerque Tessmann,Dauri José Machinski Junior,Miguel Barbosa-Tessmann,Ione Parra |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Faria,Carla Bertechini Almeida-Ferreira,Giovana Caputo Gagliardi,Karina Bertechine Alves,Tatiane Cristina Albuquerque Tessmann,Dauri José Machinski Junior,Miguel Barbosa-Tessmann,Ione Parra |
dc.subject.por.fl_str_mv |
bulgur wheat Fusarium graminearum PCR molecular detection food protection |
topic |
bulgur wheat Fusarium graminearum PCR molecular detection food protection |
description |
The detection of mycotoxigenic fungi in foodstuff is important because their presence may indicate the possible associated mycotoxin contamination. Fusarium graminearum is a wheat pathogen and a producer of micotoxins. The polymerase chain reaction (PCR) has been employed for the specific identification of F. graminearum. However, this methodology has not been commonly used for detection of F. graminearum in food. Thus, the objective of the present study was to develop a molecular methodology to detect F. graminearum in commercial samples of bulgur wheat. Two methods were tested. In the first method, a sample of this cereal was contaminated with F. graminearum mycelia. The genomic DNA was extracted from this mixture and used in a F. graminearum specific PCR reaction. The F. graminearum species was detected only in samples that were heavily contaminated. In the second method, samples of bulgur wheat were inoculated on a solid medium, and isolates having F. graminearum culture characteristics were obtained. The DNA extracted from these isolates was tested in F. graminearum specific PCR reactions. An isolate obtained had its trichothecene genotype identified by PCR. The established methodology could be used in surveys of food contamination with F. graminearum. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-03-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612012000100030 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612012000100030 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0101-20612012005000027 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Ciência e Tecnologia de Alimentos |
publisher.none.fl_str_mv |
Sociedade Brasileira de Ciência e Tecnologia de Alimentos |
dc.source.none.fl_str_mv |
Food Science and Technology v.32 n.1 2012 reponame:Food Science and Technology (Campinas) instname:Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA) instacron:SBCTA |
instname_str |
Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA) |
instacron_str |
SBCTA |
institution |
SBCTA |
reponame_str |
Food Science and Technology (Campinas) |
collection |
Food Science and Technology (Campinas) |
repository.name.fl_str_mv |
Food Science and Technology (Campinas) - Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA) |
repository.mail.fl_str_mv |
||revista@sbcta.org.br |
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1752126317340393472 |