Validation and application of an analytical method for the determination of mycotoxins in crackers by UPLC-MS/MS

Detalhes bibliográficos
Autor(a) principal: MEDINA,Bianca Gonçalves
Data de Publicação: 2019
Outros Autores: SARTORI,André Victor, MORAES,Maria Heloísa Paulino de, CARDOSO,Maria Helena Wohlers Morelli, JACOB,Silvana do Couto
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Food Science and Technology (Campinas)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612019000300583
Resumo: Abstract An analytical method applying ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was validated for the determination of aflatoxins M2, M1, G2, G1, B2, B1, deoxynivalenol, ochratoxin A, fumonisins B1 and B2, hydrolyzed fumonisins B1 and B2, zearalenone and sterigmatocystin in crackers. The obtained recoveries (70 to 110%) and relative standard deviations (< 13%) were considered satisfactory. Method limits of quantification ranged from 0.20 µg kg-1 (sterigmatocystin) to 12.27 µg kg-1 (deoxynivalenol). The validated method was then applied for mycotoxin determination in 60 cracker samples (cream crackers and water and salt crackers) obtained from the metropolitan area of the state of Rio de Janeiro, RJ. Deoxynivalenol, zearalenone and fumonisin B1 were found, respectively, in 100, 50 and 28% of the analyzed samples. The maximum permissible limits established by Brazilian legislation for deoxynivalenol and zearalenone were not exceeded in the analyzed samples.
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spelling Validation and application of an analytical method for the determination of mycotoxins in crackers by UPLC-MS/MSmycotoxinsUPLC-MS/MScrackerwheat-based biscuitsAbstract An analytical method applying ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was validated for the determination of aflatoxins M2, M1, G2, G1, B2, B1, deoxynivalenol, ochratoxin A, fumonisins B1 and B2, hydrolyzed fumonisins B1 and B2, zearalenone and sterigmatocystin in crackers. The obtained recoveries (70 to 110%) and relative standard deviations (< 13%) were considered satisfactory. Method limits of quantification ranged from 0.20 µg kg-1 (sterigmatocystin) to 12.27 µg kg-1 (deoxynivalenol). The validated method was then applied for mycotoxin determination in 60 cracker samples (cream crackers and water and salt crackers) obtained from the metropolitan area of the state of Rio de Janeiro, RJ. Deoxynivalenol, zearalenone and fumonisin B1 were found, respectively, in 100, 50 and 28% of the analyzed samples. The maximum permissible limits established by Brazilian legislation for deoxynivalenol and zearalenone were not exceeded in the analyzed samples.Sociedade Brasileira de Ciência e Tecnologia de Alimentos2019-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612019000300583Food Science and Technology v.39 n.3 2019reponame:Food Science and Technology (Campinas)instname:Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)instacron:SBCTA10.1590/fst.33717info:eu-repo/semantics/openAccessMEDINA,Bianca GonçalvesSARTORI,André VictorMORAES,Maria Heloísa Paulino deCARDOSO,Maria Helena Wohlers MorelliJACOB,Silvana do Coutoeng2019-09-20T00:00:00Zoai:scielo:S0101-20612019000300583Revistahttp://www.scielo.br/ctaONGhttps://old.scielo.br/oai/scielo-oai.php||revista@sbcta.org.br1678-457X0101-2061opendoar:2019-09-20T00:00Food Science and Technology (Campinas) - Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)false
dc.title.none.fl_str_mv Validation and application of an analytical method for the determination of mycotoxins in crackers by UPLC-MS/MS
title Validation and application of an analytical method for the determination of mycotoxins in crackers by UPLC-MS/MS
spellingShingle Validation and application of an analytical method for the determination of mycotoxins in crackers by UPLC-MS/MS
MEDINA,Bianca Gonçalves
mycotoxins
UPLC-MS/MS
cracker
wheat-based biscuits
title_short Validation and application of an analytical method for the determination of mycotoxins in crackers by UPLC-MS/MS
title_full Validation and application of an analytical method for the determination of mycotoxins in crackers by UPLC-MS/MS
title_fullStr Validation and application of an analytical method for the determination of mycotoxins in crackers by UPLC-MS/MS
title_full_unstemmed Validation and application of an analytical method for the determination of mycotoxins in crackers by UPLC-MS/MS
title_sort Validation and application of an analytical method for the determination of mycotoxins in crackers by UPLC-MS/MS
author MEDINA,Bianca Gonçalves
author_facet MEDINA,Bianca Gonçalves
SARTORI,André Victor
MORAES,Maria Heloísa Paulino de
CARDOSO,Maria Helena Wohlers Morelli
JACOB,Silvana do Couto
author_role author
author2 SARTORI,André Victor
MORAES,Maria Heloísa Paulino de
CARDOSO,Maria Helena Wohlers Morelli
JACOB,Silvana do Couto
author2_role author
author
author
author
dc.contributor.author.fl_str_mv MEDINA,Bianca Gonçalves
SARTORI,André Victor
MORAES,Maria Heloísa Paulino de
CARDOSO,Maria Helena Wohlers Morelli
JACOB,Silvana do Couto
dc.subject.por.fl_str_mv mycotoxins
UPLC-MS/MS
cracker
wheat-based biscuits
topic mycotoxins
UPLC-MS/MS
cracker
wheat-based biscuits
description Abstract An analytical method applying ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was validated for the determination of aflatoxins M2, M1, G2, G1, B2, B1, deoxynivalenol, ochratoxin A, fumonisins B1 and B2, hydrolyzed fumonisins B1 and B2, zearalenone and sterigmatocystin in crackers. The obtained recoveries (70 to 110%) and relative standard deviations (< 13%) were considered satisfactory. Method limits of quantification ranged from 0.20 µg kg-1 (sterigmatocystin) to 12.27 µg kg-1 (deoxynivalenol). The validated method was then applied for mycotoxin determination in 60 cracker samples (cream crackers and water and salt crackers) obtained from the metropolitan area of the state of Rio de Janeiro, RJ. Deoxynivalenol, zearalenone and fumonisin B1 were found, respectively, in 100, 50 and 28% of the analyzed samples. The maximum permissible limits established by Brazilian legislation for deoxynivalenol and zearalenone were not exceeded in the analyzed samples.
publishDate 2019
dc.date.none.fl_str_mv 2019-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612019000300583
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612019000300583
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/fst.33717
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Ciência e Tecnologia de Alimentos
publisher.none.fl_str_mv Sociedade Brasileira de Ciência e Tecnologia de Alimentos
dc.source.none.fl_str_mv Food Science and Technology v.39 n.3 2019
reponame:Food Science and Technology (Campinas)
instname:Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)
instacron:SBCTA
instname_str Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)
instacron_str SBCTA
institution SBCTA
reponame_str Food Science and Technology (Campinas)
collection Food Science and Technology (Campinas)
repository.name.fl_str_mv Food Science and Technology (Campinas) - Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)
repository.mail.fl_str_mv ||revista@sbcta.org.br
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