Combination of cafeteria diet with intraperitoneally streptozotocin in rats. A type-2 diabetes model
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Acta Cirúrgica Brasileira (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502021000700200 |
Resumo: | ABSTRACT Purpose To develop a model of induction of type-2 diabetes (DM2) by combining low doses of streptozotocin (STZ) and a cafeteria diet. Methods Forty male Wistar rats (200 g) were allocated into four groups: control (non-diabetic, n = 10); STZ 30 mg/kg (diabetic, n = 10); STZ 35 mg/kg (diabetic,n = 10); and STZ 40 mg/kg (diabetic, n = 10). DM2 was induced with a single intraperitoneal injection of STZ after four weeks of cafeteria diet in the three diabetic groups. All animals were evaluated as for anthropometric, and biochemical analyses, as well as liver, kidney and pancreas histological analyses. Results Lower weight gain, higher water intake, higher Lee index, hyperglycemia and modified total protein, urea, alpha-amylase, as well as insulin resistance, hepatic steatosis, pancreas, and kidney injury were observed in animals treated with 35 and 40 mg/kg of STZ. Conclusions The results show that the experimental model using cafeteria diet associated with 35 mg/kg of STZ is a low-cost model and efficient in order to develop DM2, confirmed by the presence of polydipsia, hyperglycemia, altered biochemical tests, insulin resistance and damages to the liver, pancreas and kidney, which is similar to the disease found in humans. |
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Combination of cafeteria diet with intraperitoneally streptozotocin in rats. A type-2 diabetes modelLiverHyperglycemiaBody WeightPancreasModelsAnimalABSTRACT Purpose To develop a model of induction of type-2 diabetes (DM2) by combining low doses of streptozotocin (STZ) and a cafeteria diet. Methods Forty male Wistar rats (200 g) were allocated into four groups: control (non-diabetic, n = 10); STZ 30 mg/kg (diabetic, n = 10); STZ 35 mg/kg (diabetic,n = 10); and STZ 40 mg/kg (diabetic, n = 10). DM2 was induced with a single intraperitoneal injection of STZ after four weeks of cafeteria diet in the three diabetic groups. All animals were evaluated as for anthropometric, and biochemical analyses, as well as liver, kidney and pancreas histological analyses. Results Lower weight gain, higher water intake, higher Lee index, hyperglycemia and modified total protein, urea, alpha-amylase, as well as insulin resistance, hepatic steatosis, pancreas, and kidney injury were observed in animals treated with 35 and 40 mg/kg of STZ. Conclusions The results show that the experimental model using cafeteria diet associated with 35 mg/kg of STZ is a low-cost model and efficient in order to develop DM2, confirmed by the presence of polydipsia, hyperglycemia, altered biochemical tests, insulin resistance and damages to the liver, pancreas and kidney, which is similar to the disease found in humans.Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia2021-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502021000700200Acta Cirúrgica Brasileira v.36 n.7 2021reponame:Acta Cirúrgica Brasileira (Online)instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)instacron:SBDPC10.1590/acb360702info:eu-repo/semantics/openAccessSantos,Mirelly Marques RomeiroCavalcante,Andressa Carolina Farias Pereira SubtilAmaral,Luane Aparecida doSouza,Gabriel Henrique Oliveira deSantos,Bárbara Suzuki dosPortugal,Luciane CandeloroBittencourt Junior,Felipe FransciscoTroquez,ThiagoRafacho,Bruna Paola MurinoHiane,Priscila AikoSantos,Elisvânia Freitas doseng2021-08-20T00:00:00Zoai:scielo:S0102-86502021000700200Revistahttps://www.bvs-vet.org.br/vetindex/periodicos/acta-cirurgica-brasileira/https://old.scielo.br/oai/scielo-oai.php||sgolden@terra.com.br0102-86501678-2674opendoar:2021-08-20T00:00Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)false |
dc.title.none.fl_str_mv |
Combination of cafeteria diet with intraperitoneally streptozotocin in rats. A type-2 diabetes model |
title |
Combination of cafeteria diet with intraperitoneally streptozotocin in rats. A type-2 diabetes model |
spellingShingle |
Combination of cafeteria diet with intraperitoneally streptozotocin in rats. A type-2 diabetes model Santos,Mirelly Marques Romeiro Liver Hyperglycemia Body Weight Pancreas Models Animal |
title_short |
Combination of cafeteria diet with intraperitoneally streptozotocin in rats. A type-2 diabetes model |
title_full |
Combination of cafeteria diet with intraperitoneally streptozotocin in rats. A type-2 diabetes model |
title_fullStr |
Combination of cafeteria diet with intraperitoneally streptozotocin in rats. A type-2 diabetes model |
title_full_unstemmed |
Combination of cafeteria diet with intraperitoneally streptozotocin in rats. A type-2 diabetes model |
title_sort |
Combination of cafeteria diet with intraperitoneally streptozotocin in rats. A type-2 diabetes model |
author |
Santos,Mirelly Marques Romeiro |
author_facet |
Santos,Mirelly Marques Romeiro Cavalcante,Andressa Carolina Farias Pereira Subtil Amaral,Luane Aparecida do Souza,Gabriel Henrique Oliveira de Santos,Bárbara Suzuki dos Portugal,Luciane Candeloro Bittencourt Junior,Felipe Franscisco Troquez,Thiago Rafacho,Bruna Paola Murino Hiane,Priscila Aiko Santos,Elisvânia Freitas dos |
author_role |
author |
author2 |
Cavalcante,Andressa Carolina Farias Pereira Subtil Amaral,Luane Aparecida do Souza,Gabriel Henrique Oliveira de Santos,Bárbara Suzuki dos Portugal,Luciane Candeloro Bittencourt Junior,Felipe Franscisco Troquez,Thiago Rafacho,Bruna Paola Murino Hiane,Priscila Aiko Santos,Elisvânia Freitas dos |
author2_role |
author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Santos,Mirelly Marques Romeiro Cavalcante,Andressa Carolina Farias Pereira Subtil Amaral,Luane Aparecida do Souza,Gabriel Henrique Oliveira de Santos,Bárbara Suzuki dos Portugal,Luciane Candeloro Bittencourt Junior,Felipe Franscisco Troquez,Thiago Rafacho,Bruna Paola Murino Hiane,Priscila Aiko Santos,Elisvânia Freitas dos |
dc.subject.por.fl_str_mv |
Liver Hyperglycemia Body Weight Pancreas Models Animal |
topic |
Liver Hyperglycemia Body Weight Pancreas Models Animal |
description |
ABSTRACT Purpose To develop a model of induction of type-2 diabetes (DM2) by combining low doses of streptozotocin (STZ) and a cafeteria diet. Methods Forty male Wistar rats (200 g) were allocated into four groups: control (non-diabetic, n = 10); STZ 30 mg/kg (diabetic, n = 10); STZ 35 mg/kg (diabetic,n = 10); and STZ 40 mg/kg (diabetic, n = 10). DM2 was induced with a single intraperitoneal injection of STZ after four weeks of cafeteria diet in the three diabetic groups. All animals were evaluated as for anthropometric, and biochemical analyses, as well as liver, kidney and pancreas histological analyses. Results Lower weight gain, higher water intake, higher Lee index, hyperglycemia and modified total protein, urea, alpha-amylase, as well as insulin resistance, hepatic steatosis, pancreas, and kidney injury were observed in animals treated with 35 and 40 mg/kg of STZ. Conclusions The results show that the experimental model using cafeteria diet associated with 35 mg/kg of STZ is a low-cost model and efficient in order to develop DM2, confirmed by the presence of polydipsia, hyperglycemia, altered biochemical tests, insulin resistance and damages to the liver, pancreas and kidney, which is similar to the disease found in humans. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502021000700200 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502021000700200 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/acb360702 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
publisher.none.fl_str_mv |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
dc.source.none.fl_str_mv |
Acta Cirúrgica Brasileira v.36 n.7 2021 reponame:Acta Cirúrgica Brasileira (Online) instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) instacron:SBDPC |
instname_str |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) |
instacron_str |
SBDPC |
institution |
SBDPC |
reponame_str |
Acta Cirúrgica Brasileira (Online) |
collection |
Acta Cirúrgica Brasileira (Online) |
repository.name.fl_str_mv |
Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) |
repository.mail.fl_str_mv |
||sgolden@terra.com.br |
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1752126446257569792 |