Mycobacterium massiliense BRA100 strain recovered from postsurgical infections: resistance to high concentrations of glutaraldehyde and alternative solutions for high level disinfection

• Autor(a) principal: Lorena,Nádia Suely de Oliveira
• Data de Publicação: 2010
• Outros Autores: Pitombo,Marcos Bettini, Côrtes,Patrícia Barbur, Maya,Maria Cristina Araújo, Silva,Marlei Gomes da, Carvalho,Ana Carolina da Silva, Coelho,Fábrice Santana, Miyazaki,Neide Hiromi Tokumaru, Marques,Elizabeth Andrade, Chebabo,Alberto, Freitas,Andréa D'Ávila, Lupi,Otília, Duarte,Rafael Silva
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Acta Cirúrgica Brasileira (Online)
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502010000500013
• topic: Mycobacterium Infections; Videolaparoscopy; Disinfection; Glutaraldehyde

PURPOSE: To evaluate the minimum inhibitory concentration (MIC) of GTA against these microorganisms and alternative disinfectants for high-level disinfection (HLD). METHODS: Reference mycobacteria and clinical M. massiliense strains were included in this study. Active cultures were submitted to susceptibility qualitative tests with GTA dilutions (ranging from 1.5% to 8%), and commercial orthophthaldehyde (OPA) and peracetic acid (PA) - based solutions, during the period of exposure as recommended by National Agency of Sanitary Surveillance for HLD. RESULTS: All reference and M. massiliense non-BRA100 strains, recovered from sputum, were susceptible to any GTA concentration, OPA and PA solutions. M. massiliense BRA100 strains presented MIC of 8% GTA and were susceptible to OPA and PA. CONCLUSION: M. massiliense BRA100 strain is resistant to high GTA concentrations (up to 7%), which proves that this product is non-effective against specific rapidly growing mycobacteria and should be substituted by OPA or PA - based solutions for HLD.