Effects of controlled and pressure support mechanical ventilation on rat diaphragm muscle

• Autor(a) principal: Oliveira,André de Sá Braga
• Data de Publicação: 2012
• Outros Autores: Costa,Lívia Bandeira, Assis,Thiago de Oliveira, Mota,Diógenes Luís da, França,Eduardo Ériko Tenório de, Araújo Filho,José Cândido de, Rosas,Silvania Tavares Paz, Medeiros,Paloma Lys de
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Acta Cirúrgica Brasileira (Online)
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502012000200003
• topic: Anatomy & Histology; Diaphragm; Tracheostomy; Rats, Wistar

PURPOSE: The objective of this study was to analyze the effects of Pressure Controlled Ventilation mode (PCV-C) and PSV mode in diaphragm muscle of rats. METHODS: Wistar rats (n=18) were randomly assigned to the control group or to receive 6 hours of PCV and PSV. After this period, animals were euthanized and their diaphragms were excised, frozen in liquid nitrogen and stored in at -80º C for further histomorphometric analysis. RESULTS: Results showed a 15% decrease in cross-sectional area of muscle fibers on the PCV-C group when compared to the control group (p<0.001) and by 10% when compared to the PSV group (p<0.05). Minor diameter was decreased in PCV-C group by 9% when compared with the control group (p<0.001) and by 6% when compared to the PSV group (p<0.05). When myonuclear area was analyzed, a 16% decrease was observed in the PCV-C group when compared to the PSV group (p<0.05). No significant difference between the groups was observed in myonuclear perimeter (p&gt;0.05). CONCLUSION: Short-term controlled mechanical ventilation seems to lead to muscular atrophy in diaphragm fibers. The PSV mode may attenuate the effects of VIDD.