Alternative solution for ex vivo lung perfusion, experimental study on donated human lungs non-accepted for transplantation
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Acta Cirúrgica Brasileira (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502015000500359 |
Resumo: | PURPOSE: To evaluate a new perfusate solution to be used for ex vivo lung perfusion.METHODS: Randomized experimental study using lungs from rejected brain-dead donors harvested and submitted to 1 hour of ex vivo lung perfusion (EVLP) using mainstream solution or the alternative.RESULTS: From 16 lungs blocs tested, we found no difference on weight after EVLP: Steen group (SG) = 1,097±526g; Alternative Perfusion Solution (APS) = 743±248g, p=0.163. Edema formation, assessed by Wet/dry weigh ratio, was statistically higher on the Alternative Perfusion Solution group (APS = 3.63 ± 1.26; SG = 2.06 ± 0.28; p = 0.009). No difference on PaO2 after EVLP (SG = 498±37.53mmHg; APS = 521±55.43mmHg, p=0.348, nor on histological analyses: pulmonary injury score: SG = 4.38±1.51; APS = 4.50±1.77, p=0.881; apoptotic cells count after perfusion: SG = 2.4 ± 2.0 cells/mm2; APS = 4.8 ± 6.9 cells/mm2; p = 0.361).CONCLUSION: The ex vivo lung perfusion using the alternative perfusion solution showed no functional or histological differences, except for a higher edema formation, from the EVLP using Steen Solution(r) on lungs from rejected brain-dead donors. |
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Acta Cirúrgica Brasileira (Online) |
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Alternative solution for ex vivo lung perfusion, experimental study on donated human lungs non-accepted for transplantationOrgan Preservation SolutionsLung InjuryLung TransplantationPURPOSE: To evaluate a new perfusate solution to be used for ex vivo lung perfusion.METHODS: Randomized experimental study using lungs from rejected brain-dead donors harvested and submitted to 1 hour of ex vivo lung perfusion (EVLP) using mainstream solution or the alternative.RESULTS: From 16 lungs blocs tested, we found no difference on weight after EVLP: Steen group (SG) = 1,097±526g; Alternative Perfusion Solution (APS) = 743±248g, p=0.163. Edema formation, assessed by Wet/dry weigh ratio, was statistically higher on the Alternative Perfusion Solution group (APS = 3.63 ± 1.26; SG = 2.06 ± 0.28; p = 0.009). No difference on PaO2 after EVLP (SG = 498±37.53mmHg; APS = 521±55.43mmHg, p=0.348, nor on histological analyses: pulmonary injury score: SG = 4.38±1.51; APS = 4.50±1.77, p=0.881; apoptotic cells count after perfusion: SG = 2.4 ± 2.0 cells/mm2; APS = 4.8 ± 6.9 cells/mm2; p = 0.361).CONCLUSION: The ex vivo lung perfusion using the alternative perfusion solution showed no functional or histological differences, except for a higher edema formation, from the EVLP using Steen Solution(r) on lungs from rejected brain-dead donors.Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia2015-05-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502015000500359Acta Cirúrgica Brasileira v.30 n.5 2015reponame:Acta Cirúrgica Brasileira (Online)instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)instacron:SBDPC10.1590/S0102-865020150050000008info:eu-repo/semantics/openAccessFernandes,Lucas MatosMariani,Alessandro WasumMedeiros,Israel Lopes deSamano,Marcos NaoyukiAbdalla,Luís GustavoCorreia,Aristides TadeuNepomuceno,Natália AparecidaCanzian,MauroPêgo-Fernandes,Paulo Manueleng2015-10-08T00:00:00Zoai:scielo:S0102-86502015000500359Revistahttps://www.bvs-vet.org.br/vetindex/periodicos/acta-cirurgica-brasileira/https://old.scielo.br/oai/scielo-oai.php||sgolden@terra.com.br0102-86501678-2674opendoar:2015-10-08T00:00Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)false |
dc.title.none.fl_str_mv |
Alternative solution for ex vivo lung perfusion, experimental study on donated human lungs non-accepted for transplantation |
title |
Alternative solution for ex vivo lung perfusion, experimental study on donated human lungs non-accepted for transplantation |
spellingShingle |
Alternative solution for ex vivo lung perfusion, experimental study on donated human lungs non-accepted for transplantation Fernandes,Lucas Matos Organ Preservation Solutions Lung Injury Lung Transplantation |
title_short |
Alternative solution for ex vivo lung perfusion, experimental study on donated human lungs non-accepted for transplantation |
title_full |
Alternative solution for ex vivo lung perfusion, experimental study on donated human lungs non-accepted for transplantation |
title_fullStr |
Alternative solution for ex vivo lung perfusion, experimental study on donated human lungs non-accepted for transplantation |
title_full_unstemmed |
Alternative solution for ex vivo lung perfusion, experimental study on donated human lungs non-accepted for transplantation |
title_sort |
Alternative solution for ex vivo lung perfusion, experimental study on donated human lungs non-accepted for transplantation |
author |
Fernandes,Lucas Matos |
author_facet |
Fernandes,Lucas Matos Mariani,Alessandro Wasum Medeiros,Israel Lopes de Samano,Marcos Naoyuki Abdalla,Luís Gustavo Correia,Aristides Tadeu Nepomuceno,Natália Aparecida Canzian,Mauro Pêgo-Fernandes,Paulo Manuel |
author_role |
author |
author2 |
Mariani,Alessandro Wasum Medeiros,Israel Lopes de Samano,Marcos Naoyuki Abdalla,Luís Gustavo Correia,Aristides Tadeu Nepomuceno,Natália Aparecida Canzian,Mauro Pêgo-Fernandes,Paulo Manuel |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Fernandes,Lucas Matos Mariani,Alessandro Wasum Medeiros,Israel Lopes de Samano,Marcos Naoyuki Abdalla,Luís Gustavo Correia,Aristides Tadeu Nepomuceno,Natália Aparecida Canzian,Mauro Pêgo-Fernandes,Paulo Manuel |
dc.subject.por.fl_str_mv |
Organ Preservation Solutions Lung Injury Lung Transplantation |
topic |
Organ Preservation Solutions Lung Injury Lung Transplantation |
description |
PURPOSE: To evaluate a new perfusate solution to be used for ex vivo lung perfusion.METHODS: Randomized experimental study using lungs from rejected brain-dead donors harvested and submitted to 1 hour of ex vivo lung perfusion (EVLP) using mainstream solution or the alternative.RESULTS: From 16 lungs blocs tested, we found no difference on weight after EVLP: Steen group (SG) = 1,097±526g; Alternative Perfusion Solution (APS) = 743±248g, p=0.163. Edema formation, assessed by Wet/dry weigh ratio, was statistically higher on the Alternative Perfusion Solution group (APS = 3.63 ± 1.26; SG = 2.06 ± 0.28; p = 0.009). No difference on PaO2 after EVLP (SG = 498±37.53mmHg; APS = 521±55.43mmHg, p=0.348, nor on histological analyses: pulmonary injury score: SG = 4.38±1.51; APS = 4.50±1.77, p=0.881; apoptotic cells count after perfusion: SG = 2.4 ± 2.0 cells/mm2; APS = 4.8 ± 6.9 cells/mm2; p = 0.361).CONCLUSION: The ex vivo lung perfusion using the alternative perfusion solution showed no functional or histological differences, except for a higher edema formation, from the EVLP using Steen Solution(r) on lungs from rejected brain-dead donors. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-05-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502015000500359 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502015000500359 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0102-865020150050000008 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
publisher.none.fl_str_mv |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
dc.source.none.fl_str_mv |
Acta Cirúrgica Brasileira v.30 n.5 2015 reponame:Acta Cirúrgica Brasileira (Online) instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) instacron:SBDPC |
instname_str |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) |
instacron_str |
SBDPC |
institution |
SBDPC |
reponame_str |
Acta Cirúrgica Brasileira (Online) |
collection |
Acta Cirúrgica Brasileira (Online) |
repository.name.fl_str_mv |
Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) |
repository.mail.fl_str_mv |
||sgolden@terra.com.br |
_version_ |
1752126443164270592 |