A SIMPLE EXPERIMENTAL MODEL OF HEAT SHOCK RESPONSE IN RATS

• Autor(a) principal: Meyer,Tufi Neder
• Data de Publicação: 1998
• Outros Autores: Silva,Alcino Lázaro da
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Acta Cirúrgica Brasileira (Online)
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86501998000400003
• topic: Heat-shock response; Proteins; Gene expression; Rats

Objective: To obtain a simple model for the elicitation of the heat shock response in rats. Design: Laboratory study. Setting: University research laboratories. Sample: Seventy-nine adult male albino rats (weight range 200 g to 570 g). Procedures: Exposure to heat stress by heating animals in a warm bath for 5 min after their rectal temperatures reached 107.60 F (420 C). Liver and lung samples were collected for heat-shock protein 70 (HSP70) detection (Western analysis). Results: Western analysis was positive for HSP70 in the liver and in the lungs of heated animals. There was a temporal correlation between heating and HSP70 detection: it was strongest 1 day after heating and reduced afterwards. No heated animals died. Conclusion: These data show that heating rats in a warm (45o C) bath, according to parameters set in this model, elicits efficiently the heat shock response.