Low-intensity laser therapy improves tetanic contractions in mouse anterior tibialis muscle injected with Bothrops jararaca snake venom
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Research on Biomedical Engineering (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2446-47402016000200153 |
Resumo: | Abstract Introduction Envenomation by Bothrops snakes can produce local pain, edema, hemorrhage and myonecrosis. However, standard antivenom therapy is generally ineffective in neutralizing these effects so that alternative methods of treatment have been investigated. In experimental animals, low-level laser therapy (LLLT) attenuates the local effects of Bothrops venoms, but the benefits of LLLT on muscle function after envenomation are unclear. In this study, we examined the influence of LLLT on the contractile activity of mouse skeletal muscle injected with venom from Bothrops jararaca, the principal cause of snakebite in southeastern Brazil. Methods Twenty-seven male mice were used. Mice were injected with venom (40 μg in 50 μl) in the right anterior tibialis muscle, after which the muscle tendon was exposed, connected to an isometric transducer and subjected to a resting tension of 1 g. A bipolar electrode was attached to the tibial nerve for electrical stimulation. The mice were randomly allocated to five groups: A – Control (n = 3), B – Venom 3 h (n = 6), C – Venom 9 h (n = 6), D – Venom + Laser 3 h (n = 6), E – Venom + Laser 9 h (n = 6). Results The two groups that received LLLT post-venom showed improved muscle contraction and contracture in relation to muscle treated with venom alone. Conclusion These results indicate that LLLT can improve muscle function after damage induced by B. jararaca venom. |
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Low-intensity laser therapy improves tetanic contractions in mouse anterior tibialis muscle injected with Bothrops jararaca snake venomBothrops jararacaLow-level laser therapyMuscle activityMuscle fatigueSnake venomTetanusAbstract Introduction Envenomation by Bothrops snakes can produce local pain, edema, hemorrhage and myonecrosis. However, standard antivenom therapy is generally ineffective in neutralizing these effects so that alternative methods of treatment have been investigated. In experimental animals, low-level laser therapy (LLLT) attenuates the local effects of Bothrops venoms, but the benefits of LLLT on muscle function after envenomation are unclear. In this study, we examined the influence of LLLT on the contractile activity of mouse skeletal muscle injected with venom from Bothrops jararaca, the principal cause of snakebite in southeastern Brazil. Methods Twenty-seven male mice were used. Mice were injected with venom (40 μg in 50 μl) in the right anterior tibialis muscle, after which the muscle tendon was exposed, connected to an isometric transducer and subjected to a resting tension of 1 g. A bipolar electrode was attached to the tibial nerve for electrical stimulation. The mice were randomly allocated to five groups: A – Control (n = 3), B – Venom 3 h (n = 6), C – Venom 9 h (n = 6), D – Venom + Laser 3 h (n = 6), E – Venom + Laser 9 h (n = 6). Results The two groups that received LLLT post-venom showed improved muscle contraction and contracture in relation to muscle treated with venom alone. Conclusion These results indicate that LLLT can improve muscle function after damage induced by B. jararaca venom.Sociedade Brasileira de Engenharia Biomédica2016-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S2446-47402016000200153Research on Biomedical Engineering v.32 n.2 2016reponame:Research on Biomedical Engineering (Online)instname:Sociedade Brasileira de Engenharia Biomédica (SBEB)instacron:SBEB10.1590/2446-4740.04515info:eu-repo/semantics/openAccessGiaretta,Vania Maria de AraújoSantos,Luiz PrudêncioBarbosa,Ana MariaHyslop,StephenCorrado,Alexandre PintoGalhardo,Milene SanchesNicolau,Renata AmadeiCogo,José Carloseng2016-07-21T00:00:00Zoai:scielo:S2446-47402016000200153Revistahttp://www.rbejournal.org/https://old.scielo.br/oai/scielo-oai.php||rbe@rbejournal.org2446-47402446-4732opendoar:2016-07-21T00:00Research on Biomedical Engineering (Online) - Sociedade Brasileira de Engenharia Biomédica (SBEB)false |
dc.title.none.fl_str_mv |
Low-intensity laser therapy improves tetanic contractions in mouse anterior tibialis muscle injected with Bothrops jararaca snake venom |
title |
Low-intensity laser therapy improves tetanic contractions in mouse anterior tibialis muscle injected with Bothrops jararaca snake venom |
spellingShingle |
Low-intensity laser therapy improves tetanic contractions in mouse anterior tibialis muscle injected with Bothrops jararaca snake venom Giaretta,Vania Maria de Araújo Bothrops jararaca Low-level laser therapy Muscle activity Muscle fatigue Snake venom Tetanus |
title_short |
Low-intensity laser therapy improves tetanic contractions in mouse anterior tibialis muscle injected with Bothrops jararaca snake venom |
title_full |
Low-intensity laser therapy improves tetanic contractions in mouse anterior tibialis muscle injected with Bothrops jararaca snake venom |
title_fullStr |
Low-intensity laser therapy improves tetanic contractions in mouse anterior tibialis muscle injected with Bothrops jararaca snake venom |
title_full_unstemmed |
Low-intensity laser therapy improves tetanic contractions in mouse anterior tibialis muscle injected with Bothrops jararaca snake venom |
title_sort |
Low-intensity laser therapy improves tetanic contractions in mouse anterior tibialis muscle injected with Bothrops jararaca snake venom |
author |
Giaretta,Vania Maria de Araújo |
author_facet |
Giaretta,Vania Maria de Araújo Santos,Luiz Prudêncio Barbosa,Ana Maria Hyslop,Stephen Corrado,Alexandre Pinto Galhardo,Milene Sanches Nicolau,Renata Amadei Cogo,José Carlos |
author_role |
author |
author2 |
Santos,Luiz Prudêncio Barbosa,Ana Maria Hyslop,Stephen Corrado,Alexandre Pinto Galhardo,Milene Sanches Nicolau,Renata Amadei Cogo,José Carlos |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Giaretta,Vania Maria de Araújo Santos,Luiz Prudêncio Barbosa,Ana Maria Hyslop,Stephen Corrado,Alexandre Pinto Galhardo,Milene Sanches Nicolau,Renata Amadei Cogo,José Carlos |
dc.subject.por.fl_str_mv |
Bothrops jararaca Low-level laser therapy Muscle activity Muscle fatigue Snake venom Tetanus |
topic |
Bothrops jararaca Low-level laser therapy Muscle activity Muscle fatigue Snake venom Tetanus |
description |
Abstract Introduction Envenomation by Bothrops snakes can produce local pain, edema, hemorrhage and myonecrosis. However, standard antivenom therapy is generally ineffective in neutralizing these effects so that alternative methods of treatment have been investigated. In experimental animals, low-level laser therapy (LLLT) attenuates the local effects of Bothrops venoms, but the benefits of LLLT on muscle function after envenomation are unclear. In this study, we examined the influence of LLLT on the contractile activity of mouse skeletal muscle injected with venom from Bothrops jararaca, the principal cause of snakebite in southeastern Brazil. Methods Twenty-seven male mice were used. Mice were injected with venom (40 μg in 50 μl) in the right anterior tibialis muscle, after which the muscle tendon was exposed, connected to an isometric transducer and subjected to a resting tension of 1 g. A bipolar electrode was attached to the tibial nerve for electrical stimulation. The mice were randomly allocated to five groups: A – Control (n = 3), B – Venom 3 h (n = 6), C – Venom 9 h (n = 6), D – Venom + Laser 3 h (n = 6), E – Venom + Laser 9 h (n = 6). Results The two groups that received LLLT post-venom showed improved muscle contraction and contracture in relation to muscle treated with venom alone. Conclusion These results indicate that LLLT can improve muscle function after damage induced by B. jararaca venom. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2446-47402016000200153 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2446-47402016000200153 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/2446-4740.04515 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Engenharia Biomédica |
publisher.none.fl_str_mv |
Sociedade Brasileira de Engenharia Biomédica |
dc.source.none.fl_str_mv |
Research on Biomedical Engineering v.32 n.2 2016 reponame:Research on Biomedical Engineering (Online) instname:Sociedade Brasileira de Engenharia Biomédica (SBEB) instacron:SBEB |
instname_str |
Sociedade Brasileira de Engenharia Biomédica (SBEB) |
instacron_str |
SBEB |
institution |
SBEB |
reponame_str |
Research on Biomedical Engineering (Online) |
collection |
Research on Biomedical Engineering (Online) |
repository.name.fl_str_mv |
Research on Biomedical Engineering (Online) - Sociedade Brasileira de Engenharia Biomédica (SBEB) |
repository.mail.fl_str_mv |
||rbe@rbejournal.org |
_version_ |
1752126288278061056 |