Confocal Raman spectroscopy: determination of natural moisturizing factor profile related to skin hydration

Detalhes bibliográficos
Autor(a) principal: Teixeira,Ana Paula
Data de Publicação: 2014
Outros Autores: Rangel,João Lucas, Raniero,Leandro José, Tosato,Maira Gaspar, Fávero,Priscila Pereira, Martin,Airton Abrahão
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista Brasileira de Engenharia Biomédica (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-31512014000100003
Resumo: INTRODUCTION: Skin health and skin care to reduce the effects of aging are the main interests of many researchers. The skin is very important because it protects the body from various effects of the external environment, and studies of the largest organ of the human body have been conducted since antiquity. In skin, aging effects are severe enough to promote changes in cell structure and biochemical composition. In this study, we quantitatively analyzed the water content and natural moisturizing factor of human facial skin in vivo and in real time by confocal Raman spectroscopy. This non-invasive technique is capable of providing detailed information on the biochemical composition at different depth profiles in the skin. METHODS: We studied 10 volunteers, phototype II (40 and 50 years old), using a confocal Raman system to examine the skin surface down to 25 µm. Raman spectra were obtained before product use (T0), and after 30 days of continuous use of cosmetics (T30). RESULTS: The results show a significant increase of 6.4% in water content in the surface layer of the facial skin after the cosmetic use. The amounts of natural moisturizing factor (NMF) compounds were also increased. Urocanic acid underwent a greater change in relation to carboxylic acid pyrrolidone, with a 38.5% increase in the stratum corneum. CONCLUSION: Confocal Raman spectroscopy identified changes in the biochemical composition of the superficial layers of the epidermis, which suggests the anti-aging efficacy of the formulation.
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spelling Confocal Raman spectroscopy: determination of natural moisturizing factor profile related to skin hydrationSkin agingCosmeticsConfocal Raman spectroscopyNMFINTRODUCTION: Skin health and skin care to reduce the effects of aging are the main interests of many researchers. The skin is very important because it protects the body from various effects of the external environment, and studies of the largest organ of the human body have been conducted since antiquity. In skin, aging effects are severe enough to promote changes in cell structure and biochemical composition. In this study, we quantitatively analyzed the water content and natural moisturizing factor of human facial skin in vivo and in real time by confocal Raman spectroscopy. This non-invasive technique is capable of providing detailed information on the biochemical composition at different depth profiles in the skin. METHODS: We studied 10 volunteers, phototype II (40 and 50 years old), using a confocal Raman system to examine the skin surface down to 25 µm. Raman spectra were obtained before product use (T0), and after 30 days of continuous use of cosmetics (T30). RESULTS: The results show a significant increase of 6.4% in water content in the surface layer of the facial skin after the cosmetic use. The amounts of natural moisturizing factor (NMF) compounds were also increased. Urocanic acid underwent a greater change in relation to carboxylic acid pyrrolidone, with a 38.5% increase in the stratum corneum. CONCLUSION: Confocal Raman spectroscopy identified changes in the biochemical composition of the superficial layers of the epidermis, which suggests the anti-aging efficacy of the formulation.SBEB - Sociedade Brasileira de Engenharia Biomédica2014-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-31512014000100003Revista Brasileira de Engenharia Biomédica v.30 n.1 2014reponame:Revista Brasileira de Engenharia Biomédica (Online)instname:Sociedade Brasileira de Engenharia Biomédica (SBEB)instacron:SBEB10.4322/rbeb.2014.002info:eu-repo/semantics/openAccessTeixeira,Ana PaulaRangel,João LucasRaniero,Leandro JoséTosato,Maira GasparFávero,Priscila PereiraMartin,Airton Abrahãoeng2014-04-23T00:00:00Zoai:scielo:S1517-31512014000100003Revistahttp://www.scielo.br/rbebONGhttps://old.scielo.br/oai/scielo-oai.php||rbeb@rbeb.org.br1984-77421517-3151opendoar:2014-04-23T00:00Revista Brasileira de Engenharia Biomédica (Online) - Sociedade Brasileira de Engenharia Biomédica (SBEB)false
dc.title.none.fl_str_mv Confocal Raman spectroscopy: determination of natural moisturizing factor profile related to skin hydration
title Confocal Raman spectroscopy: determination of natural moisturizing factor profile related to skin hydration
spellingShingle Confocal Raman spectroscopy: determination of natural moisturizing factor profile related to skin hydration
Teixeira,Ana Paula
Skin aging
Cosmetics
Confocal Raman spectroscopy
NMF
title_short Confocal Raman spectroscopy: determination of natural moisturizing factor profile related to skin hydration
title_full Confocal Raman spectroscopy: determination of natural moisturizing factor profile related to skin hydration
title_fullStr Confocal Raman spectroscopy: determination of natural moisturizing factor profile related to skin hydration
title_full_unstemmed Confocal Raman spectroscopy: determination of natural moisturizing factor profile related to skin hydration
title_sort Confocal Raman spectroscopy: determination of natural moisturizing factor profile related to skin hydration
author Teixeira,Ana Paula
author_facet Teixeira,Ana Paula
Rangel,João Lucas
Raniero,Leandro José
Tosato,Maira Gaspar
Fávero,Priscila Pereira
Martin,Airton Abrahão
author_role author
author2 Rangel,João Lucas
Raniero,Leandro José
Tosato,Maira Gaspar
Fávero,Priscila Pereira
Martin,Airton Abrahão
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Teixeira,Ana Paula
Rangel,João Lucas
Raniero,Leandro José
Tosato,Maira Gaspar
Fávero,Priscila Pereira
Martin,Airton Abrahão
dc.subject.por.fl_str_mv Skin aging
Cosmetics
Confocal Raman spectroscopy
NMF
topic Skin aging
Cosmetics
Confocal Raman spectroscopy
NMF
description INTRODUCTION: Skin health and skin care to reduce the effects of aging are the main interests of many researchers. The skin is very important because it protects the body from various effects of the external environment, and studies of the largest organ of the human body have been conducted since antiquity. In skin, aging effects are severe enough to promote changes in cell structure and biochemical composition. In this study, we quantitatively analyzed the water content and natural moisturizing factor of human facial skin in vivo and in real time by confocal Raman spectroscopy. This non-invasive technique is capable of providing detailed information on the biochemical composition at different depth profiles in the skin. METHODS: We studied 10 volunteers, phototype II (40 and 50 years old), using a confocal Raman system to examine the skin surface down to 25 µm. Raman spectra were obtained before product use (T0), and after 30 days of continuous use of cosmetics (T30). RESULTS: The results show a significant increase of 6.4% in water content in the surface layer of the facial skin after the cosmetic use. The amounts of natural moisturizing factor (NMF) compounds were also increased. Urocanic acid underwent a greater change in relation to carboxylic acid pyrrolidone, with a 38.5% increase in the stratum corneum. CONCLUSION: Confocal Raman spectroscopy identified changes in the biochemical composition of the superficial layers of the epidermis, which suggests the anti-aging efficacy of the formulation.
publishDate 2014
dc.date.none.fl_str_mv 2014-03-01
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dc.publisher.none.fl_str_mv SBEB - Sociedade Brasileira de Engenharia Biomédica
publisher.none.fl_str_mv SBEB - Sociedade Brasileira de Engenharia Biomédica
dc.source.none.fl_str_mv Revista Brasileira de Engenharia Biomédica v.30 n.1 2014
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