Induced defense responses in tomato against bacterial spot by proteins synthesized by endophytic bacteria

Detalhes bibliográficos
Autor(a) principal: Lanna-Filho,Roberto
Data de Publicação: 2013
Outros Autores: Souza,Ricardo M., Magalhães,Marcelo M., Villela,L., Zanotto,Edgar, Ribeiro-Júnior,Pedro M., Resende,Mário L.V.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Tropical plant pathology (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1982-56762013000400003
Resumo: Some endophytes can synthesize molecules that elicit the induction of plant resistance to infection by pathogens. The objective of this study was to demonstrate that protein fractions 42 and 75 from Bacillus amyloliquefaciens and Bacillus pumilus were capable of acting as elicitors of induced resistance in tomato plants against Xanthomonas vesicatoria, following partial resolution by gel-filtration chromatography. Tomato plants sprayed with protein fractions 42 and 75 reduced, respectively, 63.5 and 56.6% of bacterial spot, compared with control plants. Additionally, these fractions promoted the increase of peroxidase (POX) and polyphenol oxidase (PPO) enzyme activities in treated plants. In SDS-PAGE stained with silver nitrate, protein fractions 42 and 75 appeared as simple bands with estimated molecular mass of 28 and 43 kDa, respectively. We report the partial characterization of two macromolecules synthesized by endophytic bacteria that act as elicitors of systemic resistance in tomato against X. vesicatoria.
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spelling Induced defense responses in tomato against bacterial spot by proteins synthesized by endophytic bacteriaBacillus amyloliquefaciensBacillus pumilusSolanum lycopersicumXanthomonas vesicatoriabiological controlSome endophytes can synthesize molecules that elicit the induction of plant resistance to infection by pathogens. The objective of this study was to demonstrate that protein fractions 42 and 75 from Bacillus amyloliquefaciens and Bacillus pumilus were capable of acting as elicitors of induced resistance in tomato plants against Xanthomonas vesicatoria, following partial resolution by gel-filtration chromatography. Tomato plants sprayed with protein fractions 42 and 75 reduced, respectively, 63.5 and 56.6% of bacterial spot, compared with control plants. Additionally, these fractions promoted the increase of peroxidase (POX) and polyphenol oxidase (PPO) enzyme activities in treated plants. In SDS-PAGE stained with silver nitrate, protein fractions 42 and 75 appeared as simple bands with estimated molecular mass of 28 and 43 kDa, respectively. We report the partial characterization of two macromolecules synthesized by endophytic bacteria that act as elicitors of systemic resistance in tomato against X. vesicatoria.Sociedade Brasileira de Fitopatologia2013-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1982-56762013000400003Tropical Plant Pathology v.38 n.4 2013reponame:Tropical plant pathology (Online)instname:Sociedade Brasileira de Fitopatologiainstacron:SBF10.1590/S1982-56762013005000011info:eu-repo/semantics/openAccessLanna-Filho,RobertoSouza,Ricardo M.Magalhães,Marcelo M.Villela,L.Zanotto,EdgarRibeiro-Júnior,Pedro M.Resende,Mário L.V.eng2013-08-07T00:00:00Zoai:scielo:S1982-56762013000400003Revistahttps://www.scielo.br/j/tpp/ONGhttps://old.scielo.br/oai/scielo-oai.phpsbf-revista@ufla.br1983-20521982-5676opendoar:2013-08-07T00:00Tropical plant pathology (Online) - Sociedade Brasileira de Fitopatologiafalse
dc.title.none.fl_str_mv Induced defense responses in tomato against bacterial spot by proteins synthesized by endophytic bacteria
title Induced defense responses in tomato against bacterial spot by proteins synthesized by endophytic bacteria
spellingShingle Induced defense responses in tomato against bacterial spot by proteins synthesized by endophytic bacteria
Lanna-Filho,Roberto
Bacillus amyloliquefaciens
Bacillus pumilus
Solanum lycopersicum
Xanthomonas vesicatoria
biological control
title_short Induced defense responses in tomato against bacterial spot by proteins synthesized by endophytic bacteria
title_full Induced defense responses in tomato against bacterial spot by proteins synthesized by endophytic bacteria
title_fullStr Induced defense responses in tomato against bacterial spot by proteins synthesized by endophytic bacteria
title_full_unstemmed Induced defense responses in tomato against bacterial spot by proteins synthesized by endophytic bacteria
title_sort Induced defense responses in tomato against bacterial spot by proteins synthesized by endophytic bacteria
author Lanna-Filho,Roberto
author_facet Lanna-Filho,Roberto
Souza,Ricardo M.
Magalhães,Marcelo M.
Villela,L.
Zanotto,Edgar
Ribeiro-Júnior,Pedro M.
Resende,Mário L.V.
author_role author
author2 Souza,Ricardo M.
Magalhães,Marcelo M.
Villela,L.
Zanotto,Edgar
Ribeiro-Júnior,Pedro M.
Resende,Mário L.V.
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Lanna-Filho,Roberto
Souza,Ricardo M.
Magalhães,Marcelo M.
Villela,L.
Zanotto,Edgar
Ribeiro-Júnior,Pedro M.
Resende,Mário L.V.
dc.subject.por.fl_str_mv Bacillus amyloliquefaciens
Bacillus pumilus
Solanum lycopersicum
Xanthomonas vesicatoria
biological control
topic Bacillus amyloliquefaciens
Bacillus pumilus
Solanum lycopersicum
Xanthomonas vesicatoria
biological control
description Some endophytes can synthesize molecules that elicit the induction of plant resistance to infection by pathogens. The objective of this study was to demonstrate that protein fractions 42 and 75 from Bacillus amyloliquefaciens and Bacillus pumilus were capable of acting as elicitors of induced resistance in tomato plants against Xanthomonas vesicatoria, following partial resolution by gel-filtration chromatography. Tomato plants sprayed with protein fractions 42 and 75 reduced, respectively, 63.5 and 56.6% of bacterial spot, compared with control plants. Additionally, these fractions promoted the increase of peroxidase (POX) and polyphenol oxidase (PPO) enzyme activities in treated plants. In SDS-PAGE stained with silver nitrate, protein fractions 42 and 75 appeared as simple bands with estimated molecular mass of 28 and 43 kDa, respectively. We report the partial characterization of two macromolecules synthesized by endophytic bacteria that act as elicitors of systemic resistance in tomato against X. vesicatoria.
publishDate 2013
dc.date.none.fl_str_mv 2013-08-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1982-56762013000400003
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1982-56762013000400003
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1982-56762013005000011
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Fitopatologia
publisher.none.fl_str_mv Sociedade Brasileira de Fitopatologia
dc.source.none.fl_str_mv Tropical Plant Pathology v.38 n.4 2013
reponame:Tropical plant pathology (Online)
instname:Sociedade Brasileira de Fitopatologia
instacron:SBF
instname_str Sociedade Brasileira de Fitopatologia
instacron_str SBF
institution SBF
reponame_str Tropical plant pathology (Online)
collection Tropical plant pathology (Online)
repository.name.fl_str_mv Tropical plant pathology (Online) - Sociedade Brasileira de Fitopatologia
repository.mail.fl_str_mv sbf-revista@ufla.br
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