Chromosomal aberrations after induced pluripotent stem cells reprogramming
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Genetics and Molecular Biology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572021000400103 |
Resumo: | Abstract Induced pluripotent stem cells (iPSCs) are generated from adult cells that have been reprogrammed to pluripotency. However, in vitro cultivation and genetic reprogramming increase genetic instability, which could result in chromosomal abnormalities. Maintenance of genetic stability after reprogramming is required for possible experimental and clinical applications. The aim of this study was to analyze chromosomal alterations by using the G-banding karyotyping method applied to 97 samples from 38 iPSC cell lines generated from peripheral blood or Wharton’s jelly. Samples from patients with long QT syndrome, Jervell and Lange-Nielsen syndrome and amyotrophic lateral sclerosis and from normal individuals revealed the following chromosomal alterations: acentric fragments, chromosomal fusions, premature centromere divisions, double minutes, radial figures, ring chromosomes, polyploidies, inversions and trisomies. An analysis of two samples generated from Wharton’s jelly before and after reprogramming showed that abnormal clones can emerge or be selected and generate an altered lineage. IPSC lines may show clonal and nonclonal chromosomal aberrations in several passages (from P6 to P34), but these aberrations are more common in later passages. Many important chromosomal aberrations were detected, showing that G-banding is very useful for evaluating genetic instability with important repercussions for the application of iPSC lines. |
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Chromosomal aberrations after induced pluripotent stem cells reprogrammingGenetic instabilitycytogeneticsiPSCchromosomal aberrationAbstract Induced pluripotent stem cells (iPSCs) are generated from adult cells that have been reprogrammed to pluripotency. However, in vitro cultivation and genetic reprogramming increase genetic instability, which could result in chromosomal abnormalities. Maintenance of genetic stability after reprogramming is required for possible experimental and clinical applications. The aim of this study was to analyze chromosomal alterations by using the G-banding karyotyping method applied to 97 samples from 38 iPSC cell lines generated from peripheral blood or Wharton’s jelly. Samples from patients with long QT syndrome, Jervell and Lange-Nielsen syndrome and amyotrophic lateral sclerosis and from normal individuals revealed the following chromosomal alterations: acentric fragments, chromosomal fusions, premature centromere divisions, double minutes, radial figures, ring chromosomes, polyploidies, inversions and trisomies. An analysis of two samples generated from Wharton’s jelly before and after reprogramming showed that abnormal clones can emerge or be selected and generate an altered lineage. IPSC lines may show clonal and nonclonal chromosomal aberrations in several passages (from P6 to P34), but these aberrations are more common in later passages. Many important chromosomal aberrations were detected, showing that G-banding is very useful for evaluating genetic instability with important repercussions for the application of iPSC lines.Sociedade Brasileira de Genética2021-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572021000400103Genetics and Molecular Biology v.44 n.3 2021reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/1678-4685-gmb-2020-0147info:eu-repo/semantics/openAccessVaz,Isadora MayBorgonovo,TamaraKasai-Brunswick,Tais HanaeSantos,Danúbia Silva dosMesquita,Fernanda Cristina PaccolaVasques,Juliana FerreiraGubert,FernandaRebelatto,Carmen Lúcia KuniyoshiSenegaglia,Alexandra CristinaBrofman,Paulo Roberto Sludeng2021-09-01T00:00:00Zoai:scielo:S1415-47572021000400103Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2021-09-01T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false |
dc.title.none.fl_str_mv |
Chromosomal aberrations after induced pluripotent stem cells reprogramming |
title |
Chromosomal aberrations after induced pluripotent stem cells reprogramming |
spellingShingle |
Chromosomal aberrations after induced pluripotent stem cells reprogramming Vaz,Isadora May Genetic instability cytogenetics iPSC chromosomal aberration |
title_short |
Chromosomal aberrations after induced pluripotent stem cells reprogramming |
title_full |
Chromosomal aberrations after induced pluripotent stem cells reprogramming |
title_fullStr |
Chromosomal aberrations after induced pluripotent stem cells reprogramming |
title_full_unstemmed |
Chromosomal aberrations after induced pluripotent stem cells reprogramming |
title_sort |
Chromosomal aberrations after induced pluripotent stem cells reprogramming |
author |
Vaz,Isadora May |
author_facet |
Vaz,Isadora May Borgonovo,Tamara Kasai-Brunswick,Tais Hanae Santos,Danúbia Silva dos Mesquita,Fernanda Cristina Paccola Vasques,Juliana Ferreira Gubert,Fernanda Rebelatto,Carmen Lúcia Kuniyoshi Senegaglia,Alexandra Cristina Brofman,Paulo Roberto Slud |
author_role |
author |
author2 |
Borgonovo,Tamara Kasai-Brunswick,Tais Hanae Santos,Danúbia Silva dos Mesquita,Fernanda Cristina Paccola Vasques,Juliana Ferreira Gubert,Fernanda Rebelatto,Carmen Lúcia Kuniyoshi Senegaglia,Alexandra Cristina Brofman,Paulo Roberto Slud |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Vaz,Isadora May Borgonovo,Tamara Kasai-Brunswick,Tais Hanae Santos,Danúbia Silva dos Mesquita,Fernanda Cristina Paccola Vasques,Juliana Ferreira Gubert,Fernanda Rebelatto,Carmen Lúcia Kuniyoshi Senegaglia,Alexandra Cristina Brofman,Paulo Roberto Slud |
dc.subject.por.fl_str_mv |
Genetic instability cytogenetics iPSC chromosomal aberration |
topic |
Genetic instability cytogenetics iPSC chromosomal aberration |
description |
Abstract Induced pluripotent stem cells (iPSCs) are generated from adult cells that have been reprogrammed to pluripotency. However, in vitro cultivation and genetic reprogramming increase genetic instability, which could result in chromosomal abnormalities. Maintenance of genetic stability after reprogramming is required for possible experimental and clinical applications. The aim of this study was to analyze chromosomal alterations by using the G-banding karyotyping method applied to 97 samples from 38 iPSC cell lines generated from peripheral blood or Wharton’s jelly. Samples from patients with long QT syndrome, Jervell and Lange-Nielsen syndrome and amyotrophic lateral sclerosis and from normal individuals revealed the following chromosomal alterations: acentric fragments, chromosomal fusions, premature centromere divisions, double minutes, radial figures, ring chromosomes, polyploidies, inversions and trisomies. An analysis of two samples generated from Wharton’s jelly before and after reprogramming showed that abnormal clones can emerge or be selected and generate an altered lineage. IPSC lines may show clonal and nonclonal chromosomal aberrations in several passages (from P6 to P34), but these aberrations are more common in later passages. Many important chromosomal aberrations were detected, showing that G-banding is very useful for evaluating genetic instability with important repercussions for the application of iPSC lines. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572021000400103 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572021000400103 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1678-4685-gmb-2020-0147 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
dc.source.none.fl_str_mv |
Genetics and Molecular Biology v.44 n.3 2021 reponame:Genetics and Molecular Biology instname:Sociedade Brasileira de Genética (SBG) instacron:SBG |
instname_str |
Sociedade Brasileira de Genética (SBG) |
instacron_str |
SBG |
institution |
SBG |
reponame_str |
Genetics and Molecular Biology |
collection |
Genetics and Molecular Biology |
repository.name.fl_str_mv |
Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG) |
repository.mail.fl_str_mv |
||editor@gmb.org.br |
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1752122390519742464 |