The ability of mouse nuclear transfer embryonic stem cells to differentiate into primordial germ cells

Detalhes bibliográficos
Autor(a) principal: Mansouri,Vahid
Data de Publicação: 2015
Outros Autores: Salehi,Mohammad, Nourozian,Mohsen, Fadaei,Fatemeh, Farahani,Reza Mastery, Piryaei,Abbas, Delbari,Ali
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Genetics and Molecular Biology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000200220
Resumo: Nuclear transfer embryonic stem cells (ntESCs) show stem cell characteristics such as pluripotency but cause no immunological disorders. Although ntESCs are able to differentiate into somatic cells, the ability of ntESCs to differentiate into primordial germ cells (PGCs) has not been examined. In this work, we examined the capacity of mouse ntESCs to differentiate into PGCs in vitro. ntESCs aggregated to form embryoid bodies (EB) in EB culture medium supplemented with bone morphogenetic protein 4(BMP4) as the differentiation factor. The expression level of specific PGC genes was compared at days 4 and 8 using real time PCR. Flow cytometry and immunocytochemical staining were used to detect Mvh as a specific PGC marker. ntESCs expressed particular genes related to different stages of PGC development. Flow cytometry and immunocytochemical staining confirmed the presence of Mvh protein in a small number of cells. There were significant differences between cells that differentiated into PGCs in the group treated with Bmp4 compared to non-treated cells. These findings indicate that ntESCs can differentiate into putative PGCs. Improvement of ntESC differentiation into PGCs may be a reliable means of producing mature germ cells.
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spelling The ability of mouse nuclear transfer embryonic stem cells to differentiate into primordial germ cellsdifferentiationgerm cellsnuclear transfer embryonic stem cellsNuclear transfer embryonic stem cells (ntESCs) show stem cell characteristics such as pluripotency but cause no immunological disorders. Although ntESCs are able to differentiate into somatic cells, the ability of ntESCs to differentiate into primordial germ cells (PGCs) has not been examined. In this work, we examined the capacity of mouse ntESCs to differentiate into PGCs in vitro. ntESCs aggregated to form embryoid bodies (EB) in EB culture medium supplemented with bone morphogenetic protein 4(BMP4) as the differentiation factor. The expression level of specific PGC genes was compared at days 4 and 8 using real time PCR. Flow cytometry and immunocytochemical staining were used to detect Mvh as a specific PGC marker. ntESCs expressed particular genes related to different stages of PGC development. Flow cytometry and immunocytochemical staining confirmed the presence of Mvh protein in a small number of cells. There were significant differences between cells that differentiated into PGCs in the group treated with Bmp4 compared to non-treated cells. These findings indicate that ntESCs can differentiate into putative PGCs. Improvement of ntESC differentiation into PGCs may be a reliable means of producing mature germ cells.Sociedade Brasileira de Genética2015-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000200220Genetics and Molecular Biology v.38 n.2 2015reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-475738138120140213info:eu-repo/semantics/openAccessMansouri,VahidSalehi,MohammadNourozian,MohsenFadaei,FatemehFarahani,Reza MasteryPiryaei,AbbasDelbari,Alieng2015-07-31T00:00:00Zoai:scielo:S1415-47572015000200220Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2015-07-31T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false
dc.title.none.fl_str_mv The ability of mouse nuclear transfer embryonic stem cells to differentiate into primordial germ cells
title The ability of mouse nuclear transfer embryonic stem cells to differentiate into primordial germ cells
spellingShingle The ability of mouse nuclear transfer embryonic stem cells to differentiate into primordial germ cells
Mansouri,Vahid
differentiation
germ cells
nuclear transfer embryonic stem cells
title_short The ability of mouse nuclear transfer embryonic stem cells to differentiate into primordial germ cells
title_full The ability of mouse nuclear transfer embryonic stem cells to differentiate into primordial germ cells
title_fullStr The ability of mouse nuclear transfer embryonic stem cells to differentiate into primordial germ cells
title_full_unstemmed The ability of mouse nuclear transfer embryonic stem cells to differentiate into primordial germ cells
title_sort The ability of mouse nuclear transfer embryonic stem cells to differentiate into primordial germ cells
author Mansouri,Vahid
author_facet Mansouri,Vahid
Salehi,Mohammad
Nourozian,Mohsen
Fadaei,Fatemeh
Farahani,Reza Mastery
Piryaei,Abbas
Delbari,Ali
author_role author
author2 Salehi,Mohammad
Nourozian,Mohsen
Fadaei,Fatemeh
Farahani,Reza Mastery
Piryaei,Abbas
Delbari,Ali
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Mansouri,Vahid
Salehi,Mohammad
Nourozian,Mohsen
Fadaei,Fatemeh
Farahani,Reza Mastery
Piryaei,Abbas
Delbari,Ali
dc.subject.por.fl_str_mv differentiation
germ cells
nuclear transfer embryonic stem cells
topic differentiation
germ cells
nuclear transfer embryonic stem cells
description Nuclear transfer embryonic stem cells (ntESCs) show stem cell characteristics such as pluripotency but cause no immunological disorders. Although ntESCs are able to differentiate into somatic cells, the ability of ntESCs to differentiate into primordial germ cells (PGCs) has not been examined. In this work, we examined the capacity of mouse ntESCs to differentiate into PGCs in vitro. ntESCs aggregated to form embryoid bodies (EB) in EB culture medium supplemented with bone morphogenetic protein 4(BMP4) as the differentiation factor. The expression level of specific PGC genes was compared at days 4 and 8 using real time PCR. Flow cytometry and immunocytochemical staining were used to detect Mvh as a specific PGC marker. ntESCs expressed particular genes related to different stages of PGC development. Flow cytometry and immunocytochemical staining confirmed the presence of Mvh protein in a small number of cells. There were significant differences between cells that differentiated into PGCs in the group treated with Bmp4 compared to non-treated cells. These findings indicate that ntESCs can differentiate into putative PGCs. Improvement of ntESC differentiation into PGCs may be a reliable means of producing mature germ cells.
publishDate 2015
dc.date.none.fl_str_mv 2015-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000200220
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000200220
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1415-475738138120140213
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv Genetics and Molecular Biology v.38 n.2 2015
reponame:Genetics and Molecular Biology
instname:Sociedade Brasileira de Genética (SBG)
instacron:SBG
instname_str Sociedade Brasileira de Genética (SBG)
instacron_str SBG
institution SBG
reponame_str Genetics and Molecular Biology
collection Genetics and Molecular Biology
repository.name.fl_str_mv Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)
repository.mail.fl_str_mv ||editor@gmb.org.br
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