TaWRKY68 responses to biotic stresses are revealed by the orthologous genes from major cereals
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Genetics and Molecular Biology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000100013 |
Resumo: | WRKY transcription factors have been extensively characterized in the past 20 years, but in wheat, studies onWRKY genes and their function are lagging behind many other species. To explore the function of wheat WRKY genes, we identified a TaWRKY68 gene from a common wheat cultivar. It encodes a protein comprising 313 amino acids which harbors 19 conserved motifs or active sites. Gene expression patterns were determined by analyzing microarray data of TaWRKY68 in wheat and of orthologous genes from maize, rice and barley using Genevestigator. TaWRKY68 orthologs were identified and clustered using DELTA-BLAST and COBALT programs available at NCBI. The results showed that these genes, which are expressed in all tissues tested, had relatively higher levels in the roots and were up-regulated in response to biotic stresses. Bioinformatics results were confirmed by RT-PCR experiments using wheat plants infected by Agrobacterium tumefaciens and Blumeria graminis, or treated with Deoxynivalenol, a Fusarium graminearum-induced mycotoxin in wheat or barley. In summary,TaWRKY68 functions differ during plant developmental stages and might be representing a hub gene function in wheat responses to various biotic stresses. It was also found that including data from major cereal genes in the bioinformatics analysis gave more accurate and comprehensive predictions of wheat gene functions. |
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Genetics and Molecular Biology |
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TaWRKY68 responses to biotic stresses are revealed by the orthologous genes from major cerealswheatTaWRKY68Orthologous genesgenevestigatorgene functionsWRKY transcription factors have been extensively characterized in the past 20 years, but in wheat, studies onWRKY genes and their function are lagging behind many other species. To explore the function of wheat WRKY genes, we identified a TaWRKY68 gene from a common wheat cultivar. It encodes a protein comprising 313 amino acids which harbors 19 conserved motifs or active sites. Gene expression patterns were determined by analyzing microarray data of TaWRKY68 in wheat and of orthologous genes from maize, rice and barley using Genevestigator. TaWRKY68 orthologs were identified and clustered using DELTA-BLAST and COBALT programs available at NCBI. The results showed that these genes, which are expressed in all tissues tested, had relatively higher levels in the roots and were up-regulated in response to biotic stresses. Bioinformatics results were confirmed by RT-PCR experiments using wheat plants infected by Agrobacterium tumefaciens and Blumeria graminis, or treated with Deoxynivalenol, a Fusarium graminearum-induced mycotoxin in wheat or barley. In summary,TaWRKY68 functions differ during plant developmental stages and might be representing a hub gene function in wheat responses to various biotic stresses. It was also found that including data from major cereal genes in the bioinformatics analysis gave more accurate and comprehensive predictions of wheat gene functions.Sociedade Brasileira de Genética2014-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000100013Genetics and Molecular Biology v.37 n.1 2014reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47572014000100013info:eu-repo/semantics/openAccessDing,BoWang,JunbinSong,NaLi,MingCheng,QiaolinHuang,GuozhongGuo,YaolinFu,YangXie,ChaojieSun,QixinXie,Xiaodongeng2014-03-24T00:00:00Zoai:scielo:S1415-47572014000100013Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2014-03-24T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false |
dc.title.none.fl_str_mv |
TaWRKY68 responses to biotic stresses are revealed by the orthologous genes from major cereals |
title |
TaWRKY68 responses to biotic stresses are revealed by the orthologous genes from major cereals |
spellingShingle |
TaWRKY68 responses to biotic stresses are revealed by the orthologous genes from major cereals Ding,Bo wheat TaWRKY68 Orthologous genes genevestigator gene functions |
title_short |
TaWRKY68 responses to biotic stresses are revealed by the orthologous genes from major cereals |
title_full |
TaWRKY68 responses to biotic stresses are revealed by the orthologous genes from major cereals |
title_fullStr |
TaWRKY68 responses to biotic stresses are revealed by the orthologous genes from major cereals |
title_full_unstemmed |
TaWRKY68 responses to biotic stresses are revealed by the orthologous genes from major cereals |
title_sort |
TaWRKY68 responses to biotic stresses are revealed by the orthologous genes from major cereals |
author |
Ding,Bo |
author_facet |
Ding,Bo Wang,Junbin Song,Na Li,Ming Cheng,Qiaolin Huang,Guozhong Guo,Yaolin Fu,Yang Xie,Chaojie Sun,Qixin Xie,Xiaodong |
author_role |
author |
author2 |
Wang,Junbin Song,Na Li,Ming Cheng,Qiaolin Huang,Guozhong Guo,Yaolin Fu,Yang Xie,Chaojie Sun,Qixin Xie,Xiaodong |
author2_role |
author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Ding,Bo Wang,Junbin Song,Na Li,Ming Cheng,Qiaolin Huang,Guozhong Guo,Yaolin Fu,Yang Xie,Chaojie Sun,Qixin Xie,Xiaodong |
dc.subject.por.fl_str_mv |
wheat TaWRKY68 Orthologous genes genevestigator gene functions |
topic |
wheat TaWRKY68 Orthologous genes genevestigator gene functions |
description |
WRKY transcription factors have been extensively characterized in the past 20 years, but in wheat, studies onWRKY genes and their function are lagging behind many other species. To explore the function of wheat WRKY genes, we identified a TaWRKY68 gene from a common wheat cultivar. It encodes a protein comprising 313 amino acids which harbors 19 conserved motifs or active sites. Gene expression patterns were determined by analyzing microarray data of TaWRKY68 in wheat and of orthologous genes from maize, rice and barley using Genevestigator. TaWRKY68 orthologs were identified and clustered using DELTA-BLAST and COBALT programs available at NCBI. The results showed that these genes, which are expressed in all tissues tested, had relatively higher levels in the roots and were up-regulated in response to biotic stresses. Bioinformatics results were confirmed by RT-PCR experiments using wheat plants infected by Agrobacterium tumefaciens and Blumeria graminis, or treated with Deoxynivalenol, a Fusarium graminearum-induced mycotoxin in wheat or barley. In summary,TaWRKY68 functions differ during plant developmental stages and might be representing a hub gene function in wheat responses to various biotic stresses. It was also found that including data from major cereal genes in the bioinformatics analysis gave more accurate and comprehensive predictions of wheat gene functions. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000100013 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000100013 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1415-47572014000100013 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
dc.source.none.fl_str_mv |
Genetics and Molecular Biology v.37 n.1 2014 reponame:Genetics and Molecular Biology instname:Sociedade Brasileira de Genética (SBG) instacron:SBG |
instname_str |
Sociedade Brasileira de Genética (SBG) |
instacron_str |
SBG |
institution |
SBG |
reponame_str |
Genetics and Molecular Biology |
collection |
Genetics and Molecular Biology |
repository.name.fl_str_mv |
Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG) |
repository.mail.fl_str_mv |
||editor@gmb.org.br |
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1752122385830510592 |