Specific genotyping of human leukocyte antigen-A*01 by polymerase chain reaction using allele group-specific primers
Autor(a) principal: | |
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Data de Publicação: | 2006 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Genetics and Molecular Biology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200003 |
Resumo: | We established a specific genotyping assay for HLA-A*01, which is one of the most frequently found HLA-A alleles in the Caucasian population. This assay uses the polymerase chain reaction (PCR) with allele group-specific primers (ASP). HLA-A*01 group-specific primers were designed for exon 3 of the HLA-A gene, based on the recent HLA-sequence alignment. Both sense and anti-sense primers were designed with completely matched sequences to each specific HLA-A*01 allele, but mismatched by at least 1 nucleotide to all other known class I HLA alleles. By the use of these primers and stringent PCR conditions, we successfully genotyped the HLA-A*01 group alleles and achieved greater accuracy than previous methods. |
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Genetics and Molecular Biology |
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Specific genotyping of human leukocyte antigen-A*01 by polymerase chain reaction using allele group-specific primersHLA-A1PCRallele group-specific primersWe established a specific genotyping assay for HLA-A*01, which is one of the most frequently found HLA-A alleles in the Caucasian population. This assay uses the polymerase chain reaction (PCR) with allele group-specific primers (ASP). HLA-A*01 group-specific primers were designed for exon 3 of the HLA-A gene, based on the recent HLA-sequence alignment. Both sense and anti-sense primers were designed with completely matched sequences to each specific HLA-A*01 allele, but mismatched by at least 1 nucleotide to all other known class I HLA alleles. By the use of these primers and stringent PCR conditions, we successfully genotyped the HLA-A*01 group alleles and achieved greater accuracy than previous methods.Sociedade Brasileira de Genética2006-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200003Genetics and Molecular Biology v.29 n.2 2006reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47572006000200003info:eu-repo/semantics/openAccessKasuga,IkumaParé,Peter D.Sandford,Andrew J.eng2006-06-12T00:00:00Zoai:scielo:S1415-47572006000200003Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2006-06-12T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false |
dc.title.none.fl_str_mv |
Specific genotyping of human leukocyte antigen-A*01 by polymerase chain reaction using allele group-specific primers |
title |
Specific genotyping of human leukocyte antigen-A*01 by polymerase chain reaction using allele group-specific primers |
spellingShingle |
Specific genotyping of human leukocyte antigen-A*01 by polymerase chain reaction using allele group-specific primers Kasuga,Ikuma HLA-A1 PCR allele group-specific primers |
title_short |
Specific genotyping of human leukocyte antigen-A*01 by polymerase chain reaction using allele group-specific primers |
title_full |
Specific genotyping of human leukocyte antigen-A*01 by polymerase chain reaction using allele group-specific primers |
title_fullStr |
Specific genotyping of human leukocyte antigen-A*01 by polymerase chain reaction using allele group-specific primers |
title_full_unstemmed |
Specific genotyping of human leukocyte antigen-A*01 by polymerase chain reaction using allele group-specific primers |
title_sort |
Specific genotyping of human leukocyte antigen-A*01 by polymerase chain reaction using allele group-specific primers |
author |
Kasuga,Ikuma |
author_facet |
Kasuga,Ikuma Paré,Peter D. Sandford,Andrew J. |
author_role |
author |
author2 |
Paré,Peter D. Sandford,Andrew J. |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Kasuga,Ikuma Paré,Peter D. Sandford,Andrew J. |
dc.subject.por.fl_str_mv |
HLA-A1 PCR allele group-specific primers |
topic |
HLA-A1 PCR allele group-specific primers |
description |
We established a specific genotyping assay for HLA-A*01, which is one of the most frequently found HLA-A alleles in the Caucasian population. This assay uses the polymerase chain reaction (PCR) with allele group-specific primers (ASP). HLA-A*01 group-specific primers were designed for exon 3 of the HLA-A gene, based on the recent HLA-sequence alignment. Both sense and anti-sense primers were designed with completely matched sequences to each specific HLA-A*01 allele, but mismatched by at least 1 nucleotide to all other known class I HLA alleles. By the use of these primers and stringent PCR conditions, we successfully genotyped the HLA-A*01 group alleles and achieved greater accuracy than previous methods. |
publishDate |
2006 |
dc.date.none.fl_str_mv |
2006-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200003 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200003 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1415-47572006000200003 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
dc.source.none.fl_str_mv |
Genetics and Molecular Biology v.29 n.2 2006 reponame:Genetics and Molecular Biology instname:Sociedade Brasileira de Genética (SBG) instacron:SBG |
instname_str |
Sociedade Brasileira de Genética (SBG) |
instacron_str |
SBG |
institution |
SBG |
reponame_str |
Genetics and Molecular Biology |
collection |
Genetics and Molecular Biology |
repository.name.fl_str_mv |
Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG) |
repository.mail.fl_str_mv |
||editor@gmb.org.br |
_version_ |
1752122379895570432 |