PCR-RFLP analysis of non-coding regions of cpDNA in Araucaria angustifolia (Bert.) O. Kuntze
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Genetics and Molecular Biology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000300020 |
Resumo: | The Araucaria angustifolia (Bert.) O. Kuntze, also named the "paraná pine" (pinheiro-do-Paraná in Portuguese), is a native conifer species naturally occurring in the Brazilian Tropical Atlantic Forest which in Brazil is mostly limited to the southern Brazilian states of Paraná, Santa Catarina and Rio Grande do Sul. Chloroplast DNA markers (cpDNA) are useful in populational genetic studies because of their low substitution rate and the uniparental transmission. The conservation of cpDNA genes between species has allowed the design of consensus chloroplast primers that have had a great impact on population genetics and phylogenetic studies. In this study we used the polymerase chain reaction technique combined with restriction enzyme fragment length polymorphism (PCR-RFLP) to characterize the genetic diversity of the chloroplast genome in nine natural A. angustifolia populations. Among the 141 trees surveyed we found 12 different cpDNA haplotypes and demonstrated that A. angustifolia has high levels of total diversity (hT = 0.612) and an average within-population diversity (hS) of 0.441, suggesting the presence of high within-population variation. The estimated genetic divergence could be helpful in designing breeding programs and species conservation strategies, although additional studies with a larger number of populations and trees is essential for a better understanding of gene flow and the inheritance of major Araucaria angustifolia traits. |
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Genetics and Molecular Biology |
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PCR-RFLP analysis of non-coding regions of cpDNA in Araucaria angustifolia (Bert.) O. Kuntzehaplotypesconifer speciesBrazilian pinegenetic diversityThe Araucaria angustifolia (Bert.) O. Kuntze, also named the "paraná pine" (pinheiro-do-Paraná in Portuguese), is a native conifer species naturally occurring in the Brazilian Tropical Atlantic Forest which in Brazil is mostly limited to the southern Brazilian states of Paraná, Santa Catarina and Rio Grande do Sul. Chloroplast DNA markers (cpDNA) are useful in populational genetic studies because of their low substitution rate and the uniparental transmission. The conservation of cpDNA genes between species has allowed the design of consensus chloroplast primers that have had a great impact on population genetics and phylogenetic studies. In this study we used the polymerase chain reaction technique combined with restriction enzyme fragment length polymorphism (PCR-RFLP) to characterize the genetic diversity of the chloroplast genome in nine natural A. angustifolia populations. Among the 141 trees surveyed we found 12 different cpDNA haplotypes and demonstrated that A. angustifolia has high levels of total diversity (hT = 0.612) and an average within-population diversity (hS) of 0.441, suggesting the presence of high within-population variation. The estimated genetic divergence could be helpful in designing breeding programs and species conservation strategies, although additional studies with a larger number of populations and trees is essential for a better understanding of gene flow and the inheritance of major Araucaria angustifolia traits.Sociedade Brasileira de Genética2007-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000300020Genetics and Molecular Biology v.30 n.2 2007reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47572007000300020info:eu-repo/semantics/openAccessSchlögl,Paulo SérgioSouza,Anete Pereira deNodari,Rubens Onofreeng2007-06-04T00:00:00Zoai:scielo:S1415-47572007000300020Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2007-06-04T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false |
dc.title.none.fl_str_mv |
PCR-RFLP analysis of non-coding regions of cpDNA in Araucaria angustifolia (Bert.) O. Kuntze |
title |
PCR-RFLP analysis of non-coding regions of cpDNA in Araucaria angustifolia (Bert.) O. Kuntze |
spellingShingle |
PCR-RFLP analysis of non-coding regions of cpDNA in Araucaria angustifolia (Bert.) O. Kuntze Schlögl,Paulo Sérgio haplotypes conifer species Brazilian pine genetic diversity |
title_short |
PCR-RFLP analysis of non-coding regions of cpDNA in Araucaria angustifolia (Bert.) O. Kuntze |
title_full |
PCR-RFLP analysis of non-coding regions of cpDNA in Araucaria angustifolia (Bert.) O. Kuntze |
title_fullStr |
PCR-RFLP analysis of non-coding regions of cpDNA in Araucaria angustifolia (Bert.) O. Kuntze |
title_full_unstemmed |
PCR-RFLP analysis of non-coding regions of cpDNA in Araucaria angustifolia (Bert.) O. Kuntze |
title_sort |
PCR-RFLP analysis of non-coding regions of cpDNA in Araucaria angustifolia (Bert.) O. Kuntze |
author |
Schlögl,Paulo Sérgio |
author_facet |
Schlögl,Paulo Sérgio Souza,Anete Pereira de Nodari,Rubens Onofre |
author_role |
author |
author2 |
Souza,Anete Pereira de Nodari,Rubens Onofre |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Schlögl,Paulo Sérgio Souza,Anete Pereira de Nodari,Rubens Onofre |
dc.subject.por.fl_str_mv |
haplotypes conifer species Brazilian pine genetic diversity |
topic |
haplotypes conifer species Brazilian pine genetic diversity |
description |
The Araucaria angustifolia (Bert.) O. Kuntze, also named the "paraná pine" (pinheiro-do-Paraná in Portuguese), is a native conifer species naturally occurring in the Brazilian Tropical Atlantic Forest which in Brazil is mostly limited to the southern Brazilian states of Paraná, Santa Catarina and Rio Grande do Sul. Chloroplast DNA markers (cpDNA) are useful in populational genetic studies because of their low substitution rate and the uniparental transmission. The conservation of cpDNA genes between species has allowed the design of consensus chloroplast primers that have had a great impact on population genetics and phylogenetic studies. In this study we used the polymerase chain reaction technique combined with restriction enzyme fragment length polymorphism (PCR-RFLP) to characterize the genetic diversity of the chloroplast genome in nine natural A. angustifolia populations. Among the 141 trees surveyed we found 12 different cpDNA haplotypes and demonstrated that A. angustifolia has high levels of total diversity (hT = 0.612) and an average within-population diversity (hS) of 0.441, suggesting the presence of high within-population variation. The estimated genetic divergence could be helpful in designing breeding programs and species conservation strategies, although additional studies with a larger number of populations and trees is essential for a better understanding of gene flow and the inheritance of major Araucaria angustifolia traits. |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007-03-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000300020 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000300020 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1415-47572007000300020 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
dc.source.none.fl_str_mv |
Genetics and Molecular Biology v.30 n.2 2007 reponame:Genetics and Molecular Biology instname:Sociedade Brasileira de Genética (SBG) instacron:SBG |
instname_str |
Sociedade Brasileira de Genética (SBG) |
instacron_str |
SBG |
institution |
SBG |
reponame_str |
Genetics and Molecular Biology |
collection |
Genetics and Molecular Biology |
repository.name.fl_str_mv |
Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG) |
repository.mail.fl_str_mv |
||editor@gmb.org.br |
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1752122380654739456 |