DNA methylation modulates H19 and IGF2 expression in porcine female eye

Detalhes bibliográficos
Autor(a) principal: Wang,Dongxu
Data de Publicação: 2017
Outros Autores: Wang,Guodong, Yang,Hao, Liu,Haibo, Li,Cuie, Li,Xiaolan, Lin,Chao, Song,Yuning, Li,Zhanjun, Liu,Dianfeng
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Genetics and Molecular Biology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000100153
Resumo: Abstract The sexually dimorphic expression of H19/IGF2 is evolutionarily conserved. To investigate whether the expression of H19/IGF2 in the female porcine eye is sex-dependent, gene expression and methylation status were evaluated using quantitative real-time PCR (qPCR) and bisulfite sequencing PCR (BSP). We hypothesized that H19/IGF2 might exhibit a different DNA methylation status in the female eye. In order to evaluate our hypothesis, parthenogenetic (PA) cells were used for analysis by qPCR and BSP. Our results showed that H19 and IGF2 were over-expressed in the female eye compared with the male eye (3-fold and 2-fold, respectively). We observed a normal monoallelic methylation pattern for H19 differentially methylated regions (DMRs). Compared with H19 DMRs, IGF2 DMRs showed a different methylation pattern in the eye. Taken together, these results suggest that elevated expression of H19/IGF2 is caused by a specific chromatin structure that is regulated by the DNA methylation status of IGF2 DMRs in the female eye.
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spelling DNA methylation modulates H19 and IGF2 expression in porcine female eyeH19/IGF2gene expressionDNA methylationparthenogeneticpigAbstract The sexually dimorphic expression of H19/IGF2 is evolutionarily conserved. To investigate whether the expression of H19/IGF2 in the female porcine eye is sex-dependent, gene expression and methylation status were evaluated using quantitative real-time PCR (qPCR) and bisulfite sequencing PCR (BSP). We hypothesized that H19/IGF2 might exhibit a different DNA methylation status in the female eye. In order to evaluate our hypothesis, parthenogenetic (PA) cells were used for analysis by qPCR and BSP. Our results showed that H19 and IGF2 were over-expressed in the female eye compared with the male eye (3-fold and 2-fold, respectively). We observed a normal monoallelic methylation pattern for H19 differentially methylated regions (DMRs). Compared with H19 DMRs, IGF2 DMRs showed a different methylation pattern in the eye. Taken together, these results suggest that elevated expression of H19/IGF2 is caused by a specific chromatin structure that is regulated by the DNA methylation status of IGF2 DMRs in the female eye.Sociedade Brasileira de Genética2017-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000100153Genetics and Molecular Biology v.40 n.1 2017reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/1678-4685-gmb-2016-0194info:eu-repo/semantics/openAccessWang,DongxuWang,GuodongYang,HaoLiu,HaiboLi,CuieLi,XiaolanLin,ChaoSong,YuningLi,ZhanjunLiu,Dianfengeng2017-04-25T00:00:00Zoai:scielo:S1415-47572017000100153Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2017-04-25T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false
dc.title.none.fl_str_mv DNA methylation modulates H19 and IGF2 expression in porcine female eye
title DNA methylation modulates H19 and IGF2 expression in porcine female eye
spellingShingle DNA methylation modulates H19 and IGF2 expression in porcine female eye
Wang,Dongxu
H19/IGF2
gene expression
DNA methylation
parthenogenetic
pig
title_short DNA methylation modulates H19 and IGF2 expression in porcine female eye
title_full DNA methylation modulates H19 and IGF2 expression in porcine female eye
title_fullStr DNA methylation modulates H19 and IGF2 expression in porcine female eye
title_full_unstemmed DNA methylation modulates H19 and IGF2 expression in porcine female eye
title_sort DNA methylation modulates H19 and IGF2 expression in porcine female eye
author Wang,Dongxu
author_facet Wang,Dongxu
Wang,Guodong
Yang,Hao
Liu,Haibo
Li,Cuie
Li,Xiaolan
Lin,Chao
Song,Yuning
Li,Zhanjun
Liu,Dianfeng
author_role author
author2 Wang,Guodong
Yang,Hao
Liu,Haibo
Li,Cuie
Li,Xiaolan
Lin,Chao
Song,Yuning
Li,Zhanjun
Liu,Dianfeng
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Wang,Dongxu
Wang,Guodong
Yang,Hao
Liu,Haibo
Li,Cuie
Li,Xiaolan
Lin,Chao
Song,Yuning
Li,Zhanjun
Liu,Dianfeng
dc.subject.por.fl_str_mv H19/IGF2
gene expression
DNA methylation
parthenogenetic
pig
topic H19/IGF2
gene expression
DNA methylation
parthenogenetic
pig
description Abstract The sexually dimorphic expression of H19/IGF2 is evolutionarily conserved. To investigate whether the expression of H19/IGF2 in the female porcine eye is sex-dependent, gene expression and methylation status were evaluated using quantitative real-time PCR (qPCR) and bisulfite sequencing PCR (BSP). We hypothesized that H19/IGF2 might exhibit a different DNA methylation status in the female eye. In order to evaluate our hypothesis, parthenogenetic (PA) cells were used for analysis by qPCR and BSP. Our results showed that H19 and IGF2 were over-expressed in the female eye compared with the male eye (3-fold and 2-fold, respectively). We observed a normal monoallelic methylation pattern for H19 differentially methylated regions (DMRs). Compared with H19 DMRs, IGF2 DMRs showed a different methylation pattern in the eye. Taken together, these results suggest that elevated expression of H19/IGF2 is caused by a specific chromatin structure that is regulated by the DNA methylation status of IGF2 DMRs in the female eye.
publishDate 2017
dc.date.none.fl_str_mv 2017-03-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000100153
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000100153
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1678-4685-gmb-2016-0194
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv Genetics and Molecular Biology v.40 n.1 2017
reponame:Genetics and Molecular Biology
instname:Sociedade Brasileira de Genética (SBG)
instacron:SBG
instname_str Sociedade Brasileira de Genética (SBG)
instacron_str SBG
institution SBG
reponame_str Genetics and Molecular Biology
collection Genetics and Molecular Biology
repository.name.fl_str_mv Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)
repository.mail.fl_str_mv ||editor@gmb.org.br
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