Microphotometric scanning of chromatid gaps and breaks induced by AluI and BamHI in Chinese hamster ovary cells
Main Author: | |
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Publication Date: | 1996 |
Other Authors: | , , |
Format: | Article |
Language: | eng |
Source: | Brazilian Journal of Genetics |
Download full: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84551996000400007 |
Summary: | Chromatid gaps and breaks induced by the restriction endonucleases AluI and BamHI in the long arm of chromosome 1 of Chinese hamster ovary cells were microphotometrically scanned and mapped to a quantitative G-band map. More than 50% of chromatid breaks appeared as chromatin losses of greater than 5% of the total arm length. The majority of chromatid gaps and breaks as well as chromatin losses induced by both restriction endonucleases were non-randomly located in a region from 0.35 to 0.65 relative length units of the long arm of chromosome 1. We suggest that the access of these endonucleases to chromosomal DNA depends on the local organization of the chromatin. |
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Microphotometric scanning of chromatid gaps and breaks induced by AluI and BamHI in Chinese hamster ovary cellsmicrophotometric scanningchromatid gapsAluIBamHIChinese hamsterovary cellsChromatid gaps and breaks induced by the restriction endonucleases AluI and BamHI in the long arm of chromosome 1 of Chinese hamster ovary cells were microphotometrically scanned and mapped to a quantitative G-band map. More than 50% of chromatid breaks appeared as chromatin losses of greater than 5% of the total arm length. The majority of chromatid gaps and breaks as well as chromatin losses induced by both restriction endonucleases were non-randomly located in a region from 0.35 to 0.65 relative length units of the long arm of chromosome 1. We suggest that the access of these endonucleases to chromosomal DNA depends on the local organization of the chromatin.Sociedade Brasileira de Genética1996-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84551996000400007Brazilian Journal of Genetics v.19 n.4 1996reponame:Brazilian Journal of Geneticsinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S0100-84551996000400007info:eu-repo/semantics/openAccessMartínez-López,WilnerBonomi,RossanaFolle,Gustavo A.Drets,Máximo E.eng2006-10-31T00:00:00Zoai:scielo:S0100-84551996000400007Revistahttps://www.gmb.org.br/brazilian-journal-of-geneticsONGhttps://old.scielo.br/oai/scielo-oai.phpsede@sgb.org.br || sede@sgb.org.br0100-84550100-8455opendoar:2006-10-31T00:00Brazilian Journal of Genetics - Sociedade Brasileira de Genética (SBG)false |
dc.title.none.fl_str_mv |
Microphotometric scanning of chromatid gaps and breaks induced by AluI and BamHI in Chinese hamster ovary cells |
title |
Microphotometric scanning of chromatid gaps and breaks induced by AluI and BamHI in Chinese hamster ovary cells |
spellingShingle |
Microphotometric scanning of chromatid gaps and breaks induced by AluI and BamHI in Chinese hamster ovary cells Martínez-López,Wilner microphotometric scanning chromatid gaps AluI BamHI Chinese hamster ovary cells |
title_short |
Microphotometric scanning of chromatid gaps and breaks induced by AluI and BamHI in Chinese hamster ovary cells |
title_full |
Microphotometric scanning of chromatid gaps and breaks induced by AluI and BamHI in Chinese hamster ovary cells |
title_fullStr |
Microphotometric scanning of chromatid gaps and breaks induced by AluI and BamHI in Chinese hamster ovary cells |
title_full_unstemmed |
Microphotometric scanning of chromatid gaps and breaks induced by AluI and BamHI in Chinese hamster ovary cells |
title_sort |
Microphotometric scanning of chromatid gaps and breaks induced by AluI and BamHI in Chinese hamster ovary cells |
author |
Martínez-López,Wilner |
author_facet |
Martínez-López,Wilner Bonomi,Rossana Folle,Gustavo A. Drets,Máximo E. |
author_role |
author |
author2 |
Bonomi,Rossana Folle,Gustavo A. Drets,Máximo E. |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Martínez-López,Wilner Bonomi,Rossana Folle,Gustavo A. Drets,Máximo E. |
dc.subject.por.fl_str_mv |
microphotometric scanning chromatid gaps AluI BamHI Chinese hamster ovary cells |
topic |
microphotometric scanning chromatid gaps AluI BamHI Chinese hamster ovary cells |
description |
Chromatid gaps and breaks induced by the restriction endonucleases AluI and BamHI in the long arm of chromosome 1 of Chinese hamster ovary cells were microphotometrically scanned and mapped to a quantitative G-band map. More than 50% of chromatid breaks appeared as chromatin losses of greater than 5% of the total arm length. The majority of chromatid gaps and breaks as well as chromatin losses induced by both restriction endonucleases were non-randomly located in a region from 0.35 to 0.65 relative length units of the long arm of chromosome 1. We suggest that the access of these endonucleases to chromosomal DNA depends on the local organization of the chromatin. |
publishDate |
1996 |
dc.date.none.fl_str_mv |
1996-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84551996000400007 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84551996000400007 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0100-84551996000400007 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
dc.source.none.fl_str_mv |
Brazilian Journal of Genetics v.19 n.4 1996 reponame:Brazilian Journal of Genetics instname:Sociedade Brasileira de Genética (SBG) instacron:SBG |
instname_str |
Sociedade Brasileira de Genética (SBG) |
instacron_str |
SBG |
institution |
SBG |
reponame_str |
Brazilian Journal of Genetics |
collection |
Brazilian Journal of Genetics |
repository.name.fl_str_mv |
Brazilian Journal of Genetics - Sociedade Brasileira de Genética (SBG) |
repository.mail.fl_str_mv |
sede@sgb.org.br || sede@sgb.org.br |
_version_ |
1754734879152537600 |