Use of RAPD-PCR to identify true hybrid plants from crosses between closely related progenitors

Detalhes bibliográficos
Autor(a) principal: Alzate-Marin,Ana Lilia
Data de Publicação: 1996
Outros Autores: Baía,Gilson Soares, Martins Filho,Sebastião, Paula Júnior,Trazilbo José de, Sediyama,Carlos Sigueyuki, Barros,Everaldo Gonçalves de, Moreira,Maurilio Alves
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Genetics
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84551996000400016
Resumo: RAPD-PCR molecular markers were used to identify common bean and soybean hybrid plants derived from crosses between closely related progenitors, with no apparent phenotypic differences. Primers OP-F12 and OP-0O3 were used to identify true hybrids derived from crosses between common bean cultivars Rudá (A 285) and AN 910408, and soybean cultivars Cristalina and Bossier, respectively. Each primer generated one polymorphic DNA band which was present in the male progenitor and absent in the female progenitor. As RAPD bands are normally inherited as dominant characters, the presence of these bands in the F1 plants confirmed their status.
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spelling Use of RAPD-PCR to identify true hybrid plants from crosses between closely related progenitorsRAPD-PCRhybrid plantsprogenitorsRAPD-PCR molecular markers were used to identify common bean and soybean hybrid plants derived from crosses between closely related progenitors, with no apparent phenotypic differences. Primers OP-F12 and OP-0O3 were used to identify true hybrids derived from crosses between common bean cultivars Rudá (A 285) and AN 910408, and soybean cultivars Cristalina and Bossier, respectively. Each primer generated one polymorphic DNA band which was present in the male progenitor and absent in the female progenitor. As RAPD bands are normally inherited as dominant characters, the presence of these bands in the F1 plants confirmed their status.Sociedade Brasileira de Genética1996-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84551996000400016Brazilian Journal of Genetics v.19 n.4 1996reponame:Brazilian Journal of Geneticsinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S0100-84551996000400016info:eu-repo/semantics/openAccessAlzate-Marin,Ana LiliaBaía,Gilson SoaresMartins Filho,SebastiãoPaula Júnior,Trazilbo José deSediyama,Carlos SigueyukiBarros,Everaldo Gonçalves deMoreira,Maurilio Alveseng2006-10-27T00:00:00Zoai:scielo:S0100-84551996000400016Revistahttps://www.gmb.org.br/brazilian-journal-of-geneticsONGhttps://old.scielo.br/oai/scielo-oai.phpsede@sgb.org.br || sede@sgb.org.br0100-84550100-8455opendoar:2006-10-27T00:00Brazilian Journal of Genetics - Sociedade Brasileira de Genética (SBG)false
dc.title.none.fl_str_mv Use of RAPD-PCR to identify true hybrid plants from crosses between closely related progenitors
title Use of RAPD-PCR to identify true hybrid plants from crosses between closely related progenitors
spellingShingle Use of RAPD-PCR to identify true hybrid plants from crosses between closely related progenitors
Alzate-Marin,Ana Lilia
RAPD-PCR
hybrid plants
progenitors
title_short Use of RAPD-PCR to identify true hybrid plants from crosses between closely related progenitors
title_full Use of RAPD-PCR to identify true hybrid plants from crosses between closely related progenitors
title_fullStr Use of RAPD-PCR to identify true hybrid plants from crosses between closely related progenitors
title_full_unstemmed Use of RAPD-PCR to identify true hybrid plants from crosses between closely related progenitors
title_sort Use of RAPD-PCR to identify true hybrid plants from crosses between closely related progenitors
author Alzate-Marin,Ana Lilia
author_facet Alzate-Marin,Ana Lilia
Baía,Gilson Soares
Martins Filho,Sebastião
Paula Júnior,Trazilbo José de
Sediyama,Carlos Sigueyuki
Barros,Everaldo Gonçalves de
Moreira,Maurilio Alves
author_role author
author2 Baía,Gilson Soares
Martins Filho,Sebastião
Paula Júnior,Trazilbo José de
Sediyama,Carlos Sigueyuki
Barros,Everaldo Gonçalves de
Moreira,Maurilio Alves
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Alzate-Marin,Ana Lilia
Baía,Gilson Soares
Martins Filho,Sebastião
Paula Júnior,Trazilbo José de
Sediyama,Carlos Sigueyuki
Barros,Everaldo Gonçalves de
Moreira,Maurilio Alves
dc.subject.por.fl_str_mv RAPD-PCR
hybrid plants
progenitors
topic RAPD-PCR
hybrid plants
progenitors
description RAPD-PCR molecular markers were used to identify common bean and soybean hybrid plants derived from crosses between closely related progenitors, with no apparent phenotypic differences. Primers OP-F12 and OP-0O3 were used to identify true hybrids derived from crosses between common bean cultivars Rudá (A 285) and AN 910408, and soybean cultivars Cristalina and Bossier, respectively. Each primer generated one polymorphic DNA band which was present in the male progenitor and absent in the female progenitor. As RAPD bands are normally inherited as dominant characters, the presence of these bands in the F1 plants confirmed their status.
publishDate 1996
dc.date.none.fl_str_mv 1996-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84551996000400016
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84551996000400016
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0100-84551996000400016
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv Brazilian Journal of Genetics v.19 n.4 1996
reponame:Brazilian Journal of Genetics
instname:Sociedade Brasileira de Genética (SBG)
instacron:SBG
instname_str Sociedade Brasileira de Genética (SBG)
instacron_str SBG
institution SBG
reponame_str Brazilian Journal of Genetics
collection Brazilian Journal of Genetics
repository.name.fl_str_mv Brazilian Journal of Genetics - Sociedade Brasileira de Genética (SBG)
repository.mail.fl_str_mv sede@sgb.org.br || sede@sgb.org.br
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