Construction of killer industrial yeast Saccharomyces cerevisiae HAU-1 and its fermentation performance

Detalhes bibliográficos
Autor(a) principal: Bajaj,Bijender K.
Data de Publicação: 2010
Outros Autores: Sharma,S.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Microbiology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000200030
Resumo: Saccharomyces cerevisiae HAU-1, a time tested industrial yeast possesses most of the desirable fermentation characteristics like fast growth and fermentation rate, osmotolerance, high ethanol tolerance, ability to ferment molasses, and to ferment at elevated temperatures etc. However, this yeast was found to be sensitive against the killer strains of Saccharomyces cerevisiae. In the present study, killer trait was introduced into Saccharomyces cerevisiae HAU-1 by protoplast fusion with Saccharomyces cerevisiae MTCC 475, a killer strain. The resultant fusants were characterized for desirable fermentation characteristics. All the technologically important characteristics of distillery yeast Saccharomyces cerevisiae HAU-1 were retained in the fusants, and in addition the killer trait was also introduced into them. Further, the killer activity was found to be stably maintained during hostile conditions of ethanol fermentations in dextrose or molasses, and even during biomass recycling.
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spelling Construction of killer industrial yeast Saccharomyces cerevisiae HAU-1 and its fermentation performancekiller yeastdistillery yeastSaccharomyces cerevisiaemolassesethanol fermentationSaccharomyces cerevisiae HAU-1, a time tested industrial yeast possesses most of the desirable fermentation characteristics like fast growth and fermentation rate, osmotolerance, high ethanol tolerance, ability to ferment molasses, and to ferment at elevated temperatures etc. However, this yeast was found to be sensitive against the killer strains of Saccharomyces cerevisiae. In the present study, killer trait was introduced into Saccharomyces cerevisiae HAU-1 by protoplast fusion with Saccharomyces cerevisiae MTCC 475, a killer strain. The resultant fusants were characterized for desirable fermentation characteristics. All the technologically important characteristics of distillery yeast Saccharomyces cerevisiae HAU-1 were retained in the fusants, and in addition the killer trait was also introduced into them. Further, the killer activity was found to be stably maintained during hostile conditions of ethanol fermentations in dextrose or molasses, and even during biomass recycling.Sociedade Brasileira de Microbiologia2010-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000200030Brazilian Journal of Microbiology v.41 n.2 2010reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822010000200030info:eu-repo/semantics/openAccessBajaj,Bijender K.Sharma,S.eng2010-04-16T00:00:00Zoai:scielo:S1517-83822010000200030Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2010-04-16T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false
dc.title.none.fl_str_mv Construction of killer industrial yeast Saccharomyces cerevisiae HAU-1 and its fermentation performance
title Construction of killer industrial yeast Saccharomyces cerevisiae HAU-1 and its fermentation performance
spellingShingle Construction of killer industrial yeast Saccharomyces cerevisiae HAU-1 and its fermentation performance
Bajaj,Bijender K.
killer yeast
distillery yeast
Saccharomyces cerevisiae
molasses
ethanol fermentation
title_short Construction of killer industrial yeast Saccharomyces cerevisiae HAU-1 and its fermentation performance
title_full Construction of killer industrial yeast Saccharomyces cerevisiae HAU-1 and its fermentation performance
title_fullStr Construction of killer industrial yeast Saccharomyces cerevisiae HAU-1 and its fermentation performance
title_full_unstemmed Construction of killer industrial yeast Saccharomyces cerevisiae HAU-1 and its fermentation performance
title_sort Construction of killer industrial yeast Saccharomyces cerevisiae HAU-1 and its fermentation performance
author Bajaj,Bijender K.
author_facet Bajaj,Bijender K.
Sharma,S.
author_role author
author2 Sharma,S.
author2_role author
dc.contributor.author.fl_str_mv Bajaj,Bijender K.
Sharma,S.
dc.subject.por.fl_str_mv killer yeast
distillery yeast
Saccharomyces cerevisiae
molasses
ethanol fermentation
topic killer yeast
distillery yeast
Saccharomyces cerevisiae
molasses
ethanol fermentation
description Saccharomyces cerevisiae HAU-1, a time tested industrial yeast possesses most of the desirable fermentation characteristics like fast growth and fermentation rate, osmotolerance, high ethanol tolerance, ability to ferment molasses, and to ferment at elevated temperatures etc. However, this yeast was found to be sensitive against the killer strains of Saccharomyces cerevisiae. In the present study, killer trait was introduced into Saccharomyces cerevisiae HAU-1 by protoplast fusion with Saccharomyces cerevisiae MTCC 475, a killer strain. The resultant fusants were characterized for desirable fermentation characteristics. All the technologically important characteristics of distillery yeast Saccharomyces cerevisiae HAU-1 were retained in the fusants, and in addition the killer trait was also introduced into them. Further, the killer activity was found to be stably maintained during hostile conditions of ethanol fermentations in dextrose or molasses, and even during biomass recycling.
publishDate 2010
dc.date.none.fl_str_mv 2010-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000200030
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000200030
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1517-83822010000200030
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv Brazilian Journal of Microbiology v.41 n.2 2010
reponame:Brazilian Journal of Microbiology
instname:Sociedade Brasileira de Microbiologia (SBM)
instacron:SBM
instname_str Sociedade Brasileira de Microbiologia (SBM)
instacron_str SBM
institution SBM
reponame_str Brazilian Journal of Microbiology
collection Brazilian Journal of Microbiology
repository.name.fl_str_mv Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)
repository.mail.fl_str_mv bjm@sbmicrobiologia.org.br||mbmartin@usp.br
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