Polyclonal anti-intimin antibody: immunological characterization and its use in EPEC and EHEC diagnosis
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2003 |
| Outros Autores: | , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Brazilian Journal of Microbiology |
| Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500002 |
Resumo: | Intimins are outer membrane proteins expressed by enteric bacterial pathogens capable of inducing intestinal attachment-and-effacement lesion (A/E). Through immunoblotting, immunofluorescence, flow citometry and immunogold we observed that the obtained polyclonal antibody against conserved intimin region recognizes the different intimin subtypes and suggests that it can be used as a tool for EPEC and EHEC detection. Besides, immuno-dot assay seems to be a possible alternative as a capture method. |
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Polyclonal anti-intimin antibody: immunological characterization and its use in EPEC and EHEC diagnosisintiminphenotypical analysispolyclonal antibodyEPECEHECIntimins are outer membrane proteins expressed by enteric bacterial pathogens capable of inducing intestinal attachment-and-effacement lesion (A/E). Through immunoblotting, immunofluorescence, flow citometry and immunogold we observed that the obtained polyclonal antibody against conserved intimin region recognizes the different intimin subtypes and suggests that it can be used as a tool for EPEC and EHEC detection. Besides, immuno-dot assay seems to be a possible alternative as a capture method.Sociedade Brasileira de Microbiologia2003-11-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500002Brazilian Journal of Microbiology v.34 suppl.1 2003reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822003000500002info:eu-repo/semantics/openAccessKoga,Paula Célia MarikoMenezes,Caroline AnunciaçãoLima,Flávia AfonsoNara,Júlia MiticoMagalhães,Caroline ArantesCianciarullo,Aurora MarquesFerreira-Júnior,Jorge Mario da CostaTrabulsi,Luiz RachidMendes-Ledesma,Meire Roberta BrescianiPiazza,Roxane Maria Fonteseng2004-11-30T00:00:00Zoai:scielo:S1517-83822003000500002Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2004-11-30T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false |
| dc.title.none.fl_str_mv |
Polyclonal anti-intimin antibody: immunological characterization and its use in EPEC and EHEC diagnosis |
| title |
Polyclonal anti-intimin antibody: immunological characterization and its use in EPEC and EHEC diagnosis |
| spellingShingle |
Polyclonal anti-intimin antibody: immunological characterization and its use in EPEC and EHEC diagnosis Koga,Paula Célia Mariko intimin phenotypical analysis polyclonal antibody EPEC EHEC |
| title_short |
Polyclonal anti-intimin antibody: immunological characterization and its use in EPEC and EHEC diagnosis |
| title_full |
Polyclonal anti-intimin antibody: immunological characterization and its use in EPEC and EHEC diagnosis |
| title_fullStr |
Polyclonal anti-intimin antibody: immunological characterization and its use in EPEC and EHEC diagnosis |
| title_full_unstemmed |
Polyclonal anti-intimin antibody: immunological characterization and its use in EPEC and EHEC diagnosis |
| title_sort |
Polyclonal anti-intimin antibody: immunological characterization and its use in EPEC and EHEC diagnosis |
| author |
Koga,Paula Célia Mariko |
| author_facet |
Koga,Paula Célia Mariko Menezes,Caroline Anunciação Lima,Flávia Afonso Nara,Júlia Mitico Magalhães,Caroline Arantes Cianciarullo,Aurora Marques Ferreira-Júnior,Jorge Mario da Costa Trabulsi,Luiz Rachid Mendes-Ledesma,Meire Roberta Bresciani Piazza,Roxane Maria Fontes |
| author_role |
author |
| author2 |
Menezes,Caroline Anunciação Lima,Flávia Afonso Nara,Júlia Mitico Magalhães,Caroline Arantes Cianciarullo,Aurora Marques Ferreira-Júnior,Jorge Mario da Costa Trabulsi,Luiz Rachid Mendes-Ledesma,Meire Roberta Bresciani Piazza,Roxane Maria Fontes |
| author2_role |
author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Koga,Paula Célia Mariko Menezes,Caroline Anunciação Lima,Flávia Afonso Nara,Júlia Mitico Magalhães,Caroline Arantes Cianciarullo,Aurora Marques Ferreira-Júnior,Jorge Mario da Costa Trabulsi,Luiz Rachid Mendes-Ledesma,Meire Roberta Bresciani Piazza,Roxane Maria Fontes |
| dc.subject.por.fl_str_mv |
intimin phenotypical analysis polyclonal antibody EPEC EHEC |
| topic |
intimin phenotypical analysis polyclonal antibody EPEC EHEC |
| description |
Intimins are outer membrane proteins expressed by enteric bacterial pathogens capable of inducing intestinal attachment-and-effacement lesion (A/E). Through immunoblotting, immunofluorescence, flow citometry and immunogold we observed that the obtained polyclonal antibody against conserved intimin region recognizes the different intimin subtypes and suggests that it can be used as a tool for EPEC and EHEC detection. Besides, immuno-dot assay seems to be a possible alternative as a capture method. |
| publishDate |
2003 |
| dc.date.none.fl_str_mv |
2003-11-01 |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500002 |
| url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500002 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
10.1590/S1517-83822003000500002 |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
text/html |
| dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
| publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
| dc.source.none.fl_str_mv |
Brazilian Journal of Microbiology v.34 suppl.1 2003 reponame:Brazilian Journal of Microbiology instname:Sociedade Brasileira de Microbiologia (SBM) instacron:SBM |
| instname_str |
Sociedade Brasileira de Microbiologia (SBM) |
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SBM |
| institution |
SBM |
| reponame_str |
Brazilian Journal of Microbiology |
| collection |
Brazilian Journal of Microbiology |
| repository.name.fl_str_mv |
Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM) |
| repository.mail.fl_str_mv |
bjm@sbmicrobiologia.org.br||mbmartin@usp.br |
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1752122199730290688 |