Molecular approach to characterize ectomycorrhizae fungi from Mediterranean pine stands in Portugal
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Microbiology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200047 |
Resumo: | Stone pine (Pinus pinea L.), like other conifers, forms ectomycorrhizas (ECM), which have beneficial impact on plant growth in natural environments and forest ecosystems. An in vitro co-culture of stone pine microshoots with pure mycelia of isolated ECM sporocarps was used to overcome the root growth cessation not only in vitro but also to improve root development during acclimation phase. Pisolithus arhizus (Scop.) Rauschert and Lactarius deliciosus (L. ex Fr.) S.F. Gray fungi, were collected, pure cultured and used in in vitro co-culture with stone pine microshoots. Samples of P. arhizus and L. deliciosus for the in vitro co-cultures were collected from the pine stands southwest Portugal. The in situ characterization was based on their morphotypes. To confirm the identity of the collected material, ITS amplification was applied using the pure cultures derived from the sporocarps. Additionally, a molecular profile using PCR based genomic fingerprinting comparison was executed with other genera of Basidiomycetes and Ascomycetes. Our results showed the effectiveness of the techniques used to amplify DNA polymorphic sequences, which enhances the characterization of the genetic profile of ECM fungi and also provides an option to verify the fungus identity at any stage of plant mycorrhization. |
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Brazilian Journal of Microbiology |
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Molecular approach to characterize ectomycorrhizae fungi from Mediterranean pine stands in PortugalPisolithus arhizusLactarius deliciosusPinus pineaM13-PCRITSStone pine (Pinus pinea L.), like other conifers, forms ectomycorrhizas (ECM), which have beneficial impact on plant growth in natural environments and forest ecosystems. An in vitro co-culture of stone pine microshoots with pure mycelia of isolated ECM sporocarps was used to overcome the root growth cessation not only in vitro but also to improve root development during acclimation phase. Pisolithus arhizus (Scop.) Rauschert and Lactarius deliciosus (L. ex Fr.) S.F. Gray fungi, were collected, pure cultured and used in in vitro co-culture with stone pine microshoots. Samples of P. arhizus and L. deliciosus for the in vitro co-cultures were collected from the pine stands southwest Portugal. The in situ characterization was based on their morphotypes. To confirm the identity of the collected material, ITS amplification was applied using the pure cultures derived from the sporocarps. Additionally, a molecular profile using PCR based genomic fingerprinting comparison was executed with other genera of Basidiomycetes and Ascomycetes. Our results showed the effectiveness of the techniques used to amplify DNA polymorphic sequences, which enhances the characterization of the genetic profile of ECM fungi and also provides an option to verify the fungus identity at any stage of plant mycorrhization.Sociedade Brasileira de Microbiologia2013-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200047Brazilian Journal of Microbiology v.44 n.2 2013reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822013005000035info:eu-repo/semantics/openAccessRagonezi,CarlaCaldeira,A. TeresaRosário Martins,M.Salvador,CátiaSantos-Silva,CelesteGanhão,ElsaKlimaszewska,KrystynaZavattieri,Amelyeng2013-10-09T00:00:00Zoai:scielo:S1517-83822013000200047Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2013-10-09T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false |
dc.title.none.fl_str_mv |
Molecular approach to characterize ectomycorrhizae fungi from Mediterranean pine stands in Portugal |
title |
Molecular approach to characterize ectomycorrhizae fungi from Mediterranean pine stands in Portugal |
spellingShingle |
Molecular approach to characterize ectomycorrhizae fungi from Mediterranean pine stands in Portugal Ragonezi,Carla Pisolithus arhizus Lactarius deliciosus Pinus pinea M13-PCR ITS |
title_short |
Molecular approach to characterize ectomycorrhizae fungi from Mediterranean pine stands in Portugal |
title_full |
Molecular approach to characterize ectomycorrhizae fungi from Mediterranean pine stands in Portugal |
title_fullStr |
Molecular approach to characterize ectomycorrhizae fungi from Mediterranean pine stands in Portugal |
title_full_unstemmed |
Molecular approach to characterize ectomycorrhizae fungi from Mediterranean pine stands in Portugal |
title_sort |
Molecular approach to characterize ectomycorrhizae fungi from Mediterranean pine stands in Portugal |
author |
Ragonezi,Carla |
author_facet |
Ragonezi,Carla Caldeira,A. Teresa Rosário Martins,M. Salvador,Cátia Santos-Silva,Celeste Ganhão,Elsa Klimaszewska,Krystyna Zavattieri,Amely |
author_role |
author |
author2 |
Caldeira,A. Teresa Rosário Martins,M. Salvador,Cátia Santos-Silva,Celeste Ganhão,Elsa Klimaszewska,Krystyna Zavattieri,Amely |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Ragonezi,Carla Caldeira,A. Teresa Rosário Martins,M. Salvador,Cátia Santos-Silva,Celeste Ganhão,Elsa Klimaszewska,Krystyna Zavattieri,Amely |
dc.subject.por.fl_str_mv |
Pisolithus arhizus Lactarius deliciosus Pinus pinea M13-PCR ITS |
topic |
Pisolithus arhizus Lactarius deliciosus Pinus pinea M13-PCR ITS |
description |
Stone pine (Pinus pinea L.), like other conifers, forms ectomycorrhizas (ECM), which have beneficial impact on plant growth in natural environments and forest ecosystems. An in vitro co-culture of stone pine microshoots with pure mycelia of isolated ECM sporocarps was used to overcome the root growth cessation not only in vitro but also to improve root development during acclimation phase. Pisolithus arhizus (Scop.) Rauschert and Lactarius deliciosus (L. ex Fr.) S.F. Gray fungi, were collected, pure cultured and used in in vitro co-culture with stone pine microshoots. Samples of P. arhizus and L. deliciosus for the in vitro co-cultures were collected from the pine stands southwest Portugal. The in situ characterization was based on their morphotypes. To confirm the identity of the collected material, ITS amplification was applied using the pure cultures derived from the sporocarps. Additionally, a molecular profile using PCR based genomic fingerprinting comparison was executed with other genera of Basidiomycetes and Ascomycetes. Our results showed the effectiveness of the techniques used to amplify DNA polymorphic sequences, which enhances the characterization of the genetic profile of ECM fungi and also provides an option to verify the fungus identity at any stage of plant mycorrhization. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200047 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200047 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1517-83822013005000035 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
dc.source.none.fl_str_mv |
Brazilian Journal of Microbiology v.44 n.2 2013 reponame:Brazilian Journal of Microbiology instname:Sociedade Brasileira de Microbiologia (SBM) instacron:SBM |
instname_str |
Sociedade Brasileira de Microbiologia (SBM) |
instacron_str |
SBM |
institution |
SBM |
reponame_str |
Brazilian Journal of Microbiology |
collection |
Brazilian Journal of Microbiology |
repository.name.fl_str_mv |
Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM) |
repository.mail.fl_str_mv |
bjm@sbmicrobiologia.org.br||mbmartin@usp.br |
_version_ |
1752122206031183872 |