Production of polypeptide antibiotic from Streptomyces parvulus and its antibacterial activity

Detalhes bibliográficos
Autor(a) principal: Shetty,Prakasham Reddy
Data de Publicação: 2014
Outros Autores: Buddana,Sudheer Kumar, Tatipamula,Vinay Bharadwaj, Naga,Yaswanth Varanasi Venkata, Ahmad,Jamal
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Microbiology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100044
Resumo: A highly potent secondary metabolite producing actinomycetes strain is isolated from marine soil sediments of Visakhapatnam sea coast, Bay of Bengal. Over all ten strains are isolated from the collected soil sediments. Among the ten actinomycetes strains the broad spectrum strain RSPSN2 was selected for molecular characterization, antibiotic production and its purification. The nucleotide sequence of the 1 rRNA gene (1261 base pairs) of the most potent strain evidenced a 96% similarity with Streptomyces parvulus 1044 strain, Streptomyces parvulus NBRC 13193 and Streptomyces parvulus BY-F. From the taxonomic features, the actinomycetes isolate RSPSN2 matches with Streptomyces parvulus in the morphological, physiological and biochemical characters. Thus, it was given the suggested name Streptomyces parvulus RSPSN2. The active metabolite was extracted using ethyl acetate (1:3, v/v) at pH 7.0. The separation of active ingredient and its purification was performed by using both thin layer chromatography (TLC) and column chromatography (CC) techniques. Spectrometric studies such as UV-visible, FTIR, and NMR and mass were performed. The antibacterial activity of pure compound was performed by cup plate method against some pathogenic bacteria including of streptomycin resistant bacteria like (Pseudomonas mirabilis. Pseudomonas putida and Bacillus cereus). In conclusion, the collected data emphasized the fact that a polypeptide antibiotic (Actinomycin D) was produced by Streptomyces parvulus RSPSN2.
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spelling Production of polypeptide antibiotic from Streptomyces parvulus and its antibacterial activitypolypeptide antibioticStreptomyces parvulus1 rRNA characterizationantibacterial activityStreptomycin resistanceA highly potent secondary metabolite producing actinomycetes strain is isolated from marine soil sediments of Visakhapatnam sea coast, Bay of Bengal. Over all ten strains are isolated from the collected soil sediments. Among the ten actinomycetes strains the broad spectrum strain RSPSN2 was selected for molecular characterization, antibiotic production and its purification. The nucleotide sequence of the 1 rRNA gene (1261 base pairs) of the most potent strain evidenced a 96% similarity with Streptomyces parvulus 1044 strain, Streptomyces parvulus NBRC 13193 and Streptomyces parvulus BY-F. From the taxonomic features, the actinomycetes isolate RSPSN2 matches with Streptomyces parvulus in the morphological, physiological and biochemical characters. Thus, it was given the suggested name Streptomyces parvulus RSPSN2. The active metabolite was extracted using ethyl acetate (1:3, v/v) at pH 7.0. The separation of active ingredient and its purification was performed by using both thin layer chromatography (TLC) and column chromatography (CC) techniques. Spectrometric studies such as UV-visible, FTIR, and NMR and mass were performed. The antibacterial activity of pure compound was performed by cup plate method against some pathogenic bacteria including of streptomycin resistant bacteria like (Pseudomonas mirabilis. Pseudomonas putida and Bacillus cereus). In conclusion, the collected data emphasized the fact that a polypeptide antibiotic (Actinomycin D) was produced by Streptomyces parvulus RSPSN2.Sociedade Brasileira de Microbiologia2014-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100044Brazilian Journal of Microbiology v.45 n.1 2014reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822014005000022info:eu-repo/semantics/openAccessShetty,Prakasham ReddyBuddana,Sudheer KumarTatipamula,Vinay BharadwajNaga,Yaswanth Varanasi VenkataAhmad,Jamaleng2014-05-29T00:00:00Zoai:scielo:S1517-83822014000100044Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2014-05-29T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false
dc.title.none.fl_str_mv Production of polypeptide antibiotic from Streptomyces parvulus and its antibacterial activity
title Production of polypeptide antibiotic from Streptomyces parvulus and its antibacterial activity
spellingShingle Production of polypeptide antibiotic from Streptomyces parvulus and its antibacterial activity
Shetty,Prakasham Reddy
polypeptide antibiotic
Streptomyces parvulus
1 rRNA characterization
antibacterial activity
Streptomycin resistance
title_short Production of polypeptide antibiotic from Streptomyces parvulus and its antibacterial activity
title_full Production of polypeptide antibiotic from Streptomyces parvulus and its antibacterial activity
title_fullStr Production of polypeptide antibiotic from Streptomyces parvulus and its antibacterial activity
title_full_unstemmed Production of polypeptide antibiotic from Streptomyces parvulus and its antibacterial activity
title_sort Production of polypeptide antibiotic from Streptomyces parvulus and its antibacterial activity
author Shetty,Prakasham Reddy
author_facet Shetty,Prakasham Reddy
Buddana,Sudheer Kumar
Tatipamula,Vinay Bharadwaj
Naga,Yaswanth Varanasi Venkata
Ahmad,Jamal
author_role author
author2 Buddana,Sudheer Kumar
Tatipamula,Vinay Bharadwaj
Naga,Yaswanth Varanasi Venkata
Ahmad,Jamal
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Shetty,Prakasham Reddy
Buddana,Sudheer Kumar
Tatipamula,Vinay Bharadwaj
Naga,Yaswanth Varanasi Venkata
Ahmad,Jamal
dc.subject.por.fl_str_mv polypeptide antibiotic
Streptomyces parvulus
1 rRNA characterization
antibacterial activity
Streptomycin resistance
topic polypeptide antibiotic
Streptomyces parvulus
1 rRNA characterization
antibacterial activity
Streptomycin resistance
description A highly potent secondary metabolite producing actinomycetes strain is isolated from marine soil sediments of Visakhapatnam sea coast, Bay of Bengal. Over all ten strains are isolated from the collected soil sediments. Among the ten actinomycetes strains the broad spectrum strain RSPSN2 was selected for molecular characterization, antibiotic production and its purification. The nucleotide sequence of the 1 rRNA gene (1261 base pairs) of the most potent strain evidenced a 96% similarity with Streptomyces parvulus 1044 strain, Streptomyces parvulus NBRC 13193 and Streptomyces parvulus BY-F. From the taxonomic features, the actinomycetes isolate RSPSN2 matches with Streptomyces parvulus in the morphological, physiological and biochemical characters. Thus, it was given the suggested name Streptomyces parvulus RSPSN2. The active metabolite was extracted using ethyl acetate (1:3, v/v) at pH 7.0. The separation of active ingredient and its purification was performed by using both thin layer chromatography (TLC) and column chromatography (CC) techniques. Spectrometric studies such as UV-visible, FTIR, and NMR and mass were performed. The antibacterial activity of pure compound was performed by cup plate method against some pathogenic bacteria including of streptomycin resistant bacteria like (Pseudomonas mirabilis. Pseudomonas putida and Bacillus cereus). In conclusion, the collected data emphasized the fact that a polypeptide antibiotic (Actinomycin D) was produced by Streptomyces parvulus RSPSN2.
publishDate 2014
dc.date.none.fl_str_mv 2014-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100044
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100044
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1517-83822014005000022
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv Brazilian Journal of Microbiology v.45 n.1 2014
reponame:Brazilian Journal of Microbiology
instname:Sociedade Brasileira de Microbiologia (SBM)
instacron:SBM
instname_str Sociedade Brasileira de Microbiologia (SBM)
instacron_str SBM
institution SBM
reponame_str Brazilian Journal of Microbiology
collection Brazilian Journal of Microbiology
repository.name.fl_str_mv Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)
repository.mail.fl_str_mv bjm@sbmicrobiologia.org.br||mbmartin@usp.br
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