Immunoglobulin G proteolytic activity of Actinobacillus actinomycetemcomitans

Detalhes bibliográficos
Autor(a) principal: Nakanishi,Fernanda Akemi
Data de Publicação: 2006
Outros Autores: Avila-Campos,Mario Julio, Kamiji,Nádia Hizuru, Itano,Eiko Nakagawa
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Microbiology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000100008
Resumo: Actinobacillus actinomycetemcomitans produces a protease to human immunoglobulin G that is an important evasion mechanism. In this study, the proteolytic activity of A. actinomycetemcomitans strain ATCC 43718 on human immunoglobulin G associated with culture supernatant concentrations, the growth period and the period of incubation with immunoglobulin G were evaluated by an enzyme linked immunosorbent assay. The protease fraction was detected by Sephadex G 150 chromatography. The results showed that A. actinomycetemcomitans produced a protease to human immunoglobulin G in the culture supernatant, and the highest activity was achieved witen the concentration was 27.5 mug protein/mL, after culturing for 72 hours and incubating with IgG for 24 hours. The molecular mass of the protease active fraction was from 43 to 150 kDa.
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spelling Immunoglobulin G proteolytic activity of Actinobacillus actinomycetemcomitansimmunoglobulin GActinobacillus actinomycetemcomitansELISAproteaseActinobacillus actinomycetemcomitans produces a protease to human immunoglobulin G that is an important evasion mechanism. In this study, the proteolytic activity of A. actinomycetemcomitans strain ATCC 43718 on human immunoglobulin G associated with culture supernatant concentrations, the growth period and the period of incubation with immunoglobulin G were evaluated by an enzyme linked immunosorbent assay. The protease fraction was detected by Sephadex G 150 chromatography. The results showed that A. actinomycetemcomitans produced a protease to human immunoglobulin G in the culture supernatant, and the highest activity was achieved witen the concentration was 27.5 mug protein/mL, after culturing for 72 hours and incubating with IgG for 24 hours. The molecular mass of the protease active fraction was from 43 to 150 kDa.Sociedade Brasileira de Microbiologia2006-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000100008Brazilian Journal of Microbiology v.37 n.1 2006reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822006000100008info:eu-repo/semantics/openAccessNakanishi,Fernanda AkemiAvila-Campos,Mario JulioKamiji,Nádia HizuruItano,Eiko Nakagawaeng2006-05-19T00:00:00Zoai:scielo:S1517-83822006000100008Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2006-05-19T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false
dc.title.none.fl_str_mv Immunoglobulin G proteolytic activity of Actinobacillus actinomycetemcomitans
title Immunoglobulin G proteolytic activity of Actinobacillus actinomycetemcomitans
spellingShingle Immunoglobulin G proteolytic activity of Actinobacillus actinomycetemcomitans
Nakanishi,Fernanda Akemi
immunoglobulin G
Actinobacillus actinomycetemcomitans
ELISA
protease
title_short Immunoglobulin G proteolytic activity of Actinobacillus actinomycetemcomitans
title_full Immunoglobulin G proteolytic activity of Actinobacillus actinomycetemcomitans
title_fullStr Immunoglobulin G proteolytic activity of Actinobacillus actinomycetemcomitans
title_full_unstemmed Immunoglobulin G proteolytic activity of Actinobacillus actinomycetemcomitans
title_sort Immunoglobulin G proteolytic activity of Actinobacillus actinomycetemcomitans
author Nakanishi,Fernanda Akemi
author_facet Nakanishi,Fernanda Akemi
Avila-Campos,Mario Julio
Kamiji,Nádia Hizuru
Itano,Eiko Nakagawa
author_role author
author2 Avila-Campos,Mario Julio
Kamiji,Nádia Hizuru
Itano,Eiko Nakagawa
author2_role author
author
author
dc.contributor.author.fl_str_mv Nakanishi,Fernanda Akemi
Avila-Campos,Mario Julio
Kamiji,Nádia Hizuru
Itano,Eiko Nakagawa
dc.subject.por.fl_str_mv immunoglobulin G
Actinobacillus actinomycetemcomitans
ELISA
protease
topic immunoglobulin G
Actinobacillus actinomycetemcomitans
ELISA
protease
description Actinobacillus actinomycetemcomitans produces a protease to human immunoglobulin G that is an important evasion mechanism. In this study, the proteolytic activity of A. actinomycetemcomitans strain ATCC 43718 on human immunoglobulin G associated with culture supernatant concentrations, the growth period and the period of incubation with immunoglobulin G were evaluated by an enzyme linked immunosorbent assay. The protease fraction was detected by Sephadex G 150 chromatography. The results showed that A. actinomycetemcomitans produced a protease to human immunoglobulin G in the culture supernatant, and the highest activity was achieved witen the concentration was 27.5 mug protein/mL, after culturing for 72 hours and incubating with IgG for 24 hours. The molecular mass of the protease active fraction was from 43 to 150 kDa.
publishDate 2006
dc.date.none.fl_str_mv 2006-03-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000100008
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000100008
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1517-83822006000100008
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv Brazilian Journal of Microbiology v.37 n.1 2006
reponame:Brazilian Journal of Microbiology
instname:Sociedade Brasileira de Microbiologia (SBM)
instacron:SBM
instname_str Sociedade Brasileira de Microbiologia (SBM)
instacron_str SBM
institution SBM
reponame_str Brazilian Journal of Microbiology
collection Brazilian Journal of Microbiology
repository.name.fl_str_mv Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)
repository.mail.fl_str_mv bjm@sbmicrobiologia.org.br||mbmartin@usp.br
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