Detection of mixed microbial biofilms on central venous catheters removed from Intensive care Unit Patients
Autor(a) principal: | |
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Data de Publicação: | 2005 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Microbiology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000300013 |
Resumo: | Central venous catheters from intensive care unit patients were subjected to microbiological methods (semiquantitative culture) and scanning electron microscopy in order to assess microbial attachment and correlate it with blood cultures. During the period of the survey, 59 patients with inserted central venous catheters were studied. The type of catheter used was nontunneled, noncuffed, single lumen, made of polyurethane. Blood samples for cultures were collected at the moment of catheter removal. Data on the patient's age, gender, catheter insertion site, and duration of catheterization were also obtained. From 63 catheters tips analysed, 30 (47.6%) showed microbial colonization. Infection proved to be more prevalent in 26 (41.3%) patients with catheters inserted via subclavia vein than in 2 (3.2%) inserted via the jugular vein. Infection was observed more frequently in catheters which were kept in place more than seven days. A. baumannii, Citrobacter freundii, E. aerogenes, P. aeruginosa and S. saprohyticus were isolated as causal agents of catheter-related bloodstream infections. The antimicrobial agent with greater in vitro activity against Gram-negative bacteria was imipenen and against Gram-positive were vancomycin, cefepime, penicillin, rifampin and tetracycline. The SEM analyses revealed biofilms on surfaces of all the catheters examined. |
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Detection of mixed microbial biofilms on central venous catheters removed from Intensive care Unit Patientsbiofilmbacteremia-cathetercentral venous cathetermicroorganisms attachmentCentral venous catheters from intensive care unit patients were subjected to microbiological methods (semiquantitative culture) and scanning electron microscopy in order to assess microbial attachment and correlate it with blood cultures. During the period of the survey, 59 patients with inserted central venous catheters were studied. The type of catheter used was nontunneled, noncuffed, single lumen, made of polyurethane. Blood samples for cultures were collected at the moment of catheter removal. Data on the patient's age, gender, catheter insertion site, and duration of catheterization were also obtained. From 63 catheters tips analysed, 30 (47.6%) showed microbial colonization. Infection proved to be more prevalent in 26 (41.3%) patients with catheters inserted via subclavia vein than in 2 (3.2%) inserted via the jugular vein. Infection was observed more frequently in catheters which were kept in place more than seven days. A. baumannii, Citrobacter freundii, E. aerogenes, P. aeruginosa and S. saprohyticus were isolated as causal agents of catheter-related bloodstream infections. The antimicrobial agent with greater in vitro activity against Gram-negative bacteria was imipenen and against Gram-positive were vancomycin, cefepime, penicillin, rifampin and tetracycline. The SEM analyses revealed biofilms on surfaces of all the catheters examined.Sociedade Brasileira de Microbiologia2005-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000300013Brazilian Journal of Microbiology v.36 n.3 2005reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822005000300013info:eu-repo/semantics/openAccessStorti,AnisioPizzolitto,Antonio CarlosPizzolitto,Elisabeth Loshchagineng2006-02-20T00:00:00Zoai:scielo:S1517-83822005000300013Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2006-02-20T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false |
dc.title.none.fl_str_mv |
Detection of mixed microbial biofilms on central venous catheters removed from Intensive care Unit Patients |
title |
Detection of mixed microbial biofilms on central venous catheters removed from Intensive care Unit Patients |
spellingShingle |
Detection of mixed microbial biofilms on central venous catheters removed from Intensive care Unit Patients Storti,Anisio biofilm bacteremia-catheter central venous catheter microorganisms attachment |
title_short |
Detection of mixed microbial biofilms on central venous catheters removed from Intensive care Unit Patients |
title_full |
Detection of mixed microbial biofilms on central venous catheters removed from Intensive care Unit Patients |
title_fullStr |
Detection of mixed microbial biofilms on central venous catheters removed from Intensive care Unit Patients |
title_full_unstemmed |
Detection of mixed microbial biofilms on central venous catheters removed from Intensive care Unit Patients |
title_sort |
Detection of mixed microbial biofilms on central venous catheters removed from Intensive care Unit Patients |
author |
Storti,Anisio |
author_facet |
Storti,Anisio Pizzolitto,Antonio Carlos Pizzolitto,Elisabeth Loshchagin |
author_role |
author |
author2 |
Pizzolitto,Antonio Carlos Pizzolitto,Elisabeth Loshchagin |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Storti,Anisio Pizzolitto,Antonio Carlos Pizzolitto,Elisabeth Loshchagin |
dc.subject.por.fl_str_mv |
biofilm bacteremia-catheter central venous catheter microorganisms attachment |
topic |
biofilm bacteremia-catheter central venous catheter microorganisms attachment |
description |
Central venous catheters from intensive care unit patients were subjected to microbiological methods (semiquantitative culture) and scanning electron microscopy in order to assess microbial attachment and correlate it with blood cultures. During the period of the survey, 59 patients with inserted central venous catheters were studied. The type of catheter used was nontunneled, noncuffed, single lumen, made of polyurethane. Blood samples for cultures were collected at the moment of catheter removal. Data on the patient's age, gender, catheter insertion site, and duration of catheterization were also obtained. From 63 catheters tips analysed, 30 (47.6%) showed microbial colonization. Infection proved to be more prevalent in 26 (41.3%) patients with catheters inserted via subclavia vein than in 2 (3.2%) inserted via the jugular vein. Infection was observed more frequently in catheters which were kept in place more than seven days. A. baumannii, Citrobacter freundii, E. aerogenes, P. aeruginosa and S. saprohyticus were isolated as causal agents of catheter-related bloodstream infections. The antimicrobial agent with greater in vitro activity against Gram-negative bacteria was imipenen and against Gram-positive were vancomycin, cefepime, penicillin, rifampin and tetracycline. The SEM analyses revealed biofilms on surfaces of all the catheters examined. |
publishDate |
2005 |
dc.date.none.fl_str_mv |
2005-09-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000300013 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000300013 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1517-83822005000300013 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
dc.source.none.fl_str_mv |
Brazilian Journal of Microbiology v.36 n.3 2005 reponame:Brazilian Journal of Microbiology instname:Sociedade Brasileira de Microbiologia (SBM) instacron:SBM |
instname_str |
Sociedade Brasileira de Microbiologia (SBM) |
instacron_str |
SBM |
institution |
SBM |
reponame_str |
Brazilian Journal of Microbiology |
collection |
Brazilian Journal of Microbiology |
repository.name.fl_str_mv |
Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM) |
repository.mail.fl_str_mv |
bjm@sbmicrobiologia.org.br||mbmartin@usp.br |
_version_ |
1752122200491556864 |