EXPERIMENTAL MODEL OF CANDIDA ALBICANS (SEROTYPES A AND B) ADHERENCE IN VITRO

Detalhes bibliográficos
Autor(a) principal: Pires,Maria de Fátima Costa
Data de Publicação: 2001
Outros Autores: Corrêa,Benedito, Gambale,Walderez, Paula,Claudete Rodrigues
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Microbiology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000300001
Resumo: The adherence of Candida albicans is considered an important virulence factor since it determines the colonization of the yeast in various regions of the host, being the initial cause of the infection. In this work we studied the adherence of C. albicans (serotypes A and B) to HeLa and Vero cells after culture for 18 h and 30 h in chemically defined medium (YNB) plus carbohydrate sources such as glucose, sucrose, maltose, galactose and mannose (500 mM). Each assay was performed in triplicate. The best results were obtained with the medium containing glucose. The adherence was greatest in 18 h cultures at 37ºC and at 1 h contact time. The interaction of yeast cells with the host cells was studied by scanning microscopy.
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spelling EXPERIMENTAL MODEL OF CANDIDA ALBICANS (SEROTYPES A AND B) ADHERENCE IN VITROCandida albicansadherenceserotypesThe adherence of Candida albicans is considered an important virulence factor since it determines the colonization of the yeast in various regions of the host, being the initial cause of the infection. In this work we studied the adherence of C. albicans (serotypes A and B) to HeLa and Vero cells after culture for 18 h and 30 h in chemically defined medium (YNB) plus carbohydrate sources such as glucose, sucrose, maltose, galactose and mannose (500 mM). Each assay was performed in triplicate. The best results were obtained with the medium containing glucose. The adherence was greatest in 18 h cultures at 37ºC and at 1 h contact time. The interaction of yeast cells with the host cells was studied by scanning microscopy.Sociedade Brasileira de Microbiologia2001-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000300001Brazilian Journal of Microbiology v.32 n.3 2001reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822001000300001info:eu-repo/semantics/openAccessPires,Maria de Fátima CostaCorrêa,BeneditoGambale,WalderezPaula,Claudete Rodrigueseng2002-04-04T00:00:00Zoai:scielo:S1517-83822001000300001Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2002-04-04T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false
dc.title.none.fl_str_mv EXPERIMENTAL MODEL OF CANDIDA ALBICANS (SEROTYPES A AND B) ADHERENCE IN VITRO
title EXPERIMENTAL MODEL OF CANDIDA ALBICANS (SEROTYPES A AND B) ADHERENCE IN VITRO
spellingShingle EXPERIMENTAL MODEL OF CANDIDA ALBICANS (SEROTYPES A AND B) ADHERENCE IN VITRO
Pires,Maria de Fátima Costa
Candida albicans
adherence
serotypes
title_short EXPERIMENTAL MODEL OF CANDIDA ALBICANS (SEROTYPES A AND B) ADHERENCE IN VITRO
title_full EXPERIMENTAL MODEL OF CANDIDA ALBICANS (SEROTYPES A AND B) ADHERENCE IN VITRO
title_fullStr EXPERIMENTAL MODEL OF CANDIDA ALBICANS (SEROTYPES A AND B) ADHERENCE IN VITRO
title_full_unstemmed EXPERIMENTAL MODEL OF CANDIDA ALBICANS (SEROTYPES A AND B) ADHERENCE IN VITRO
title_sort EXPERIMENTAL MODEL OF CANDIDA ALBICANS (SEROTYPES A AND B) ADHERENCE IN VITRO
author Pires,Maria de Fátima Costa
author_facet Pires,Maria de Fátima Costa
Corrêa,Benedito
Gambale,Walderez
Paula,Claudete Rodrigues
author_role author
author2 Corrêa,Benedito
Gambale,Walderez
Paula,Claudete Rodrigues
author2_role author
author
author
dc.contributor.author.fl_str_mv Pires,Maria de Fátima Costa
Corrêa,Benedito
Gambale,Walderez
Paula,Claudete Rodrigues
dc.subject.por.fl_str_mv Candida albicans
adherence
serotypes
topic Candida albicans
adherence
serotypes
description The adherence of Candida albicans is considered an important virulence factor since it determines the colonization of the yeast in various regions of the host, being the initial cause of the infection. In this work we studied the adherence of C. albicans (serotypes A and B) to HeLa and Vero cells after culture for 18 h and 30 h in chemically defined medium (YNB) plus carbohydrate sources such as glucose, sucrose, maltose, galactose and mannose (500 mM). Each assay was performed in triplicate. The best results were obtained with the medium containing glucose. The adherence was greatest in 18 h cultures at 37ºC and at 1 h contact time. The interaction of yeast cells with the host cells was studied by scanning microscopy.
publishDate 2001
dc.date.none.fl_str_mv 2001-10-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000300001
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000300001
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1517-83822001000300001
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv Brazilian Journal of Microbiology v.32 n.3 2001
reponame:Brazilian Journal of Microbiology
instname:Sociedade Brasileira de Microbiologia (SBM)
instacron:SBM
instname_str Sociedade Brasileira de Microbiologia (SBM)
instacron_str SBM
institution SBM
reponame_str Brazilian Journal of Microbiology
collection Brazilian Journal of Microbiology
repository.name.fl_str_mv Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)
repository.mail.fl_str_mv bjm@sbmicrobiologia.org.br||mbmartin@usp.br
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