Campylobacter in broiler slaughter samples assessed by direct count on mCCDA and Campy-Cefex agar
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Microbiology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000300764 |
Resumo: | ABSTRACT Campylobacter spp. cause foodborne illnesses in humans primarily through the consumption of contaminated chicken. The aim of this study was to evaluate the United States Department of Agriculture's (USDA) recommended methodology, protocol MLG 41.02, for the isolation, identification and direct plate counting of Campylobacter jejuni and C. coli samples from the broiler slaughtering process. A plating method using both mCCDA and Campy-Cefex agars is recommended to recover Campylobacter cells. It is also possible to use this method in different matrices (cloacal swabs and water samples). Cloacal swabs, samples from pre-chiller and post-chiller carcasses and samples of pre-chiller, chiller and direct supply water were collected each week for four weeks from the same flock at a slaughterhouse located in an abattoir in southern Brazil. Samples were analyzed to directly count Campylobacter spp., and the results showed a high frequency of Campylobacter spp. on Campy-Cefex agar. For the isolated species, 72% were identified as Campylobacter jejuni and 38% as Campylobacter coli. It was possible to count Campylobacter jejuni and Campylobacter coli from different samples, including the water supply samples, using the two-agar method. These results suggest that slaughterhouses can use direct counting methods with both agars and different matrices as a monitoring tool to assess the presence of Campylobacter bacteria in their products. |
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Brazilian Journal of Microbiology |
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Campylobacter in broiler slaughter samples assessed by direct count on mCCDA and Campy-Cefex agarCampylobacterAgar plate countmCCDACampy-CefexBroilerABSTRACT Campylobacter spp. cause foodborne illnesses in humans primarily through the consumption of contaminated chicken. The aim of this study was to evaluate the United States Department of Agriculture's (USDA) recommended methodology, protocol MLG 41.02, for the isolation, identification and direct plate counting of Campylobacter jejuni and C. coli samples from the broiler slaughtering process. A plating method using both mCCDA and Campy-Cefex agars is recommended to recover Campylobacter cells. It is also possible to use this method in different matrices (cloacal swabs and water samples). Cloacal swabs, samples from pre-chiller and post-chiller carcasses and samples of pre-chiller, chiller and direct supply water were collected each week for four weeks from the same flock at a slaughterhouse located in an abattoir in southern Brazil. Samples were analyzed to directly count Campylobacter spp., and the results showed a high frequency of Campylobacter spp. on Campy-Cefex agar. For the isolated species, 72% were identified as Campylobacter jejuni and 38% as Campylobacter coli. It was possible to count Campylobacter jejuni and Campylobacter coli from different samples, including the water supply samples, using the two-agar method. These results suggest that slaughterhouses can use direct counting methods with both agars and different matrices as a monitoring tool to assess the presence of Campylobacter bacteria in their products.Sociedade Brasileira de Microbiologia2016-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000300764Brazilian Journal of Microbiology v.47 n.3 2016reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1016/j.bjm.2016.04.025info:eu-repo/semantics/openAccessGonsalves,Camila CristinaBorsoi,AnderlisePerdoncini,GustavoRodrigues,Laura BeatrizNascimento,Vladimir Pinheiro doeng2016-08-02T00:00:00Zoai:scielo:S1517-83822016000300764Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2016-08-02T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false |
dc.title.none.fl_str_mv |
Campylobacter in broiler slaughter samples assessed by direct count on mCCDA and Campy-Cefex agar |
title |
Campylobacter in broiler slaughter samples assessed by direct count on mCCDA and Campy-Cefex agar |
spellingShingle |
Campylobacter in broiler slaughter samples assessed by direct count on mCCDA and Campy-Cefex agar Gonsalves,Camila Cristina Campylobacter Agar plate count mCCDA Campy-Cefex Broiler |
title_short |
Campylobacter in broiler slaughter samples assessed by direct count on mCCDA and Campy-Cefex agar |
title_full |
Campylobacter in broiler slaughter samples assessed by direct count on mCCDA and Campy-Cefex agar |
title_fullStr |
Campylobacter in broiler slaughter samples assessed by direct count on mCCDA and Campy-Cefex agar |
title_full_unstemmed |
Campylobacter in broiler slaughter samples assessed by direct count on mCCDA and Campy-Cefex agar |
title_sort |
Campylobacter in broiler slaughter samples assessed by direct count on mCCDA and Campy-Cefex agar |
author |
Gonsalves,Camila Cristina |
author_facet |
Gonsalves,Camila Cristina Borsoi,Anderlise Perdoncini,Gustavo Rodrigues,Laura Beatriz Nascimento,Vladimir Pinheiro do |
author_role |
author |
author2 |
Borsoi,Anderlise Perdoncini,Gustavo Rodrigues,Laura Beatriz Nascimento,Vladimir Pinheiro do |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Gonsalves,Camila Cristina Borsoi,Anderlise Perdoncini,Gustavo Rodrigues,Laura Beatriz Nascimento,Vladimir Pinheiro do |
dc.subject.por.fl_str_mv |
Campylobacter Agar plate count mCCDA Campy-Cefex Broiler |
topic |
Campylobacter Agar plate count mCCDA Campy-Cefex Broiler |
description |
ABSTRACT Campylobacter spp. cause foodborne illnesses in humans primarily through the consumption of contaminated chicken. The aim of this study was to evaluate the United States Department of Agriculture's (USDA) recommended methodology, protocol MLG 41.02, for the isolation, identification and direct plate counting of Campylobacter jejuni and C. coli samples from the broiler slaughtering process. A plating method using both mCCDA and Campy-Cefex agars is recommended to recover Campylobacter cells. It is also possible to use this method in different matrices (cloacal swabs and water samples). Cloacal swabs, samples from pre-chiller and post-chiller carcasses and samples of pre-chiller, chiller and direct supply water were collected each week for four weeks from the same flock at a slaughterhouse located in an abattoir in southern Brazil. Samples were analyzed to directly count Campylobacter spp., and the results showed a high frequency of Campylobacter spp. on Campy-Cefex agar. For the isolated species, 72% were identified as Campylobacter jejuni and 38% as Campylobacter coli. It was possible to count Campylobacter jejuni and Campylobacter coli from different samples, including the water supply samples, using the two-agar method. These results suggest that slaughterhouses can use direct counting methods with both agars and different matrices as a monitoring tool to assess the presence of Campylobacter bacteria in their products. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-09-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000300764 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000300764 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1016/j.bjm.2016.04.025 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
dc.source.none.fl_str_mv |
Brazilian Journal of Microbiology v.47 n.3 2016 reponame:Brazilian Journal of Microbiology instname:Sociedade Brasileira de Microbiologia (SBM) instacron:SBM |
instname_str |
Sociedade Brasileira de Microbiologia (SBM) |
instacron_str |
SBM |
institution |
SBM |
reponame_str |
Brazilian Journal of Microbiology |
collection |
Brazilian Journal of Microbiology |
repository.name.fl_str_mv |
Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM) |
repository.mail.fl_str_mv |
bjm@sbmicrobiologia.org.br||mbmartin@usp.br |
_version_ |
1752122208467025920 |