Membrane permeabilization of colistin toward pan-drug resistant Gram-negative isolates
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Microbiology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000200381 |
Resumo: | Abstract Pan-drug resistant Gram-negative bacteria, being resistant to most available antibiotics, represent a huge threat to the medical community. Colistin is considered the last therapeutic option for patients in hospital settings. Thus, we were concerned in this study to demonstrate the membrane permeabilizing activity of colistin focusing on investigating its efficiency toward those pan-drug resistant isolates which represent a critical situation. We determined the killing dynamics of colistin against pan-drug resistant isolates. The permeability alteration was confirmed by different techniques as: leakage, electron microscopy and construction of an artificial membrane model; liposomes. Moreover, selectivity of colistin against microbial cells was also elucidated. Colistin was proved to be rapid bactericidal against pan-drug resistant isolates. It interacts with the outer bacterial membrane leading to deformation of its outline, pore formation, leakage of internal contents, cell lysis and finally death. Furthermore, variations in membrane composition of eukaryotic and microbial cells provide a key for colistin selectivity toward bacterial cells. Colistin selectively alters membrane permeability of pan-drug resistant isolates which leads to cell lysis. Colistin was proved to be an efficient last line treatment for pan-drug resistant infections which are hard to treat. |
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Brazilian Journal of Microbiology |
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Membrane permeabilization of colistin toward pan-drug resistant Gram-negative isolatesPan-drug resistantLeakagePermeability alterationColistinUltrastructureAbstract Pan-drug resistant Gram-negative bacteria, being resistant to most available antibiotics, represent a huge threat to the medical community. Colistin is considered the last therapeutic option for patients in hospital settings. Thus, we were concerned in this study to demonstrate the membrane permeabilizing activity of colistin focusing on investigating its efficiency toward those pan-drug resistant isolates which represent a critical situation. We determined the killing dynamics of colistin against pan-drug resistant isolates. The permeability alteration was confirmed by different techniques as: leakage, electron microscopy and construction of an artificial membrane model; liposomes. Moreover, selectivity of colistin against microbial cells was also elucidated. Colistin was proved to be rapid bactericidal against pan-drug resistant isolates. It interacts with the outer bacterial membrane leading to deformation of its outline, pore formation, leakage of internal contents, cell lysis and finally death. Furthermore, variations in membrane composition of eukaryotic and microbial cells provide a key for colistin selectivity toward bacterial cells. Colistin selectively alters membrane permeability of pan-drug resistant isolates which leads to cell lysis. Colistin was proved to be an efficient last line treatment for pan-drug resistant infections which are hard to treat.Sociedade Brasileira de Microbiologia2016-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000200381Brazilian Journal of Microbiology v.47 n.2 2016reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1016/j.bjm.2016.01.007info:eu-repo/semantics/openAccessMohamed,Yasmine FathyAbou-Shleib,Hamida MoustafaKhalil,Amal MohamedEl-Guink,Nadia MohamedEl-Nakeeb,Moustafa Ahmedeng2016-05-03T00:00:00Zoai:scielo:S1517-83822016000200381Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2016-05-03T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false |
dc.title.none.fl_str_mv |
Membrane permeabilization of colistin toward pan-drug resistant Gram-negative isolates |
title |
Membrane permeabilization of colistin toward pan-drug resistant Gram-negative isolates |
spellingShingle |
Membrane permeabilization of colistin toward pan-drug resistant Gram-negative isolates Mohamed,Yasmine Fathy Pan-drug resistant Leakage Permeability alteration Colistin Ultrastructure |
title_short |
Membrane permeabilization of colistin toward pan-drug resistant Gram-negative isolates |
title_full |
Membrane permeabilization of colistin toward pan-drug resistant Gram-negative isolates |
title_fullStr |
Membrane permeabilization of colistin toward pan-drug resistant Gram-negative isolates |
title_full_unstemmed |
Membrane permeabilization of colistin toward pan-drug resistant Gram-negative isolates |
title_sort |
Membrane permeabilization of colistin toward pan-drug resistant Gram-negative isolates |
author |
Mohamed,Yasmine Fathy |
author_facet |
Mohamed,Yasmine Fathy Abou-Shleib,Hamida Moustafa Khalil,Amal Mohamed El-Guink,Nadia Mohamed El-Nakeeb,Moustafa Ahmed |
author_role |
author |
author2 |
Abou-Shleib,Hamida Moustafa Khalil,Amal Mohamed El-Guink,Nadia Mohamed El-Nakeeb,Moustafa Ahmed |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Mohamed,Yasmine Fathy Abou-Shleib,Hamida Moustafa Khalil,Amal Mohamed El-Guink,Nadia Mohamed El-Nakeeb,Moustafa Ahmed |
dc.subject.por.fl_str_mv |
Pan-drug resistant Leakage Permeability alteration Colistin Ultrastructure |
topic |
Pan-drug resistant Leakage Permeability alteration Colistin Ultrastructure |
description |
Abstract Pan-drug resistant Gram-negative bacteria, being resistant to most available antibiotics, represent a huge threat to the medical community. Colistin is considered the last therapeutic option for patients in hospital settings. Thus, we were concerned in this study to demonstrate the membrane permeabilizing activity of colistin focusing on investigating its efficiency toward those pan-drug resistant isolates which represent a critical situation. We determined the killing dynamics of colistin against pan-drug resistant isolates. The permeability alteration was confirmed by different techniques as: leakage, electron microscopy and construction of an artificial membrane model; liposomes. Moreover, selectivity of colistin against microbial cells was also elucidated. Colistin was proved to be rapid bactericidal against pan-drug resistant isolates. It interacts with the outer bacterial membrane leading to deformation of its outline, pore formation, leakage of internal contents, cell lysis and finally death. Furthermore, variations in membrane composition of eukaryotic and microbial cells provide a key for colistin selectivity toward bacterial cells. Colistin selectively alters membrane permeability of pan-drug resistant isolates which leads to cell lysis. Colistin was proved to be an efficient last line treatment for pan-drug resistant infections which are hard to treat. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000200381 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000200381 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1016/j.bjm.2016.01.007 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
dc.source.none.fl_str_mv |
Brazilian Journal of Microbiology v.47 n.2 2016 reponame:Brazilian Journal of Microbiology instname:Sociedade Brasileira de Microbiologia (SBM) instacron:SBM |
instname_str |
Sociedade Brasileira de Microbiologia (SBM) |
instacron_str |
SBM |
institution |
SBM |
reponame_str |
Brazilian Journal of Microbiology |
collection |
Brazilian Journal of Microbiology |
repository.name.fl_str_mv |
Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM) |
repository.mail.fl_str_mv |
bjm@sbmicrobiologia.org.br||mbmartin@usp.br |
_version_ |
1752122208371605504 |