Detection of different Staphylococcus aureus strains in bovine milk from subclinical mastitis using PCR and routine techniques
Autor(a) principal: | |
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Data de Publicação: | 2001 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Microbiology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000100007 |
Resumo: | Contamination of fresh milk with Staphylococcus aureus was assessed comparatively through routine phenotypic (coagulase tube test and coagulase slide test) and genotypic (PCR) screening of 128 S. aureus strains isolated from 555 milk samples. These samples were collected from 362 cows with subclinical mastitis, hosted in different dairy herds at various locations of the Northern and Northeastern rural areas of the State of Rio de Janeiro, 39.7% of which were CMT-positive. All S. aureus isolates tested positive for the presence of the coagulase gene by PCR and the isolates could be grouped into four distinct classes according to the size of the PCR product. The strains also yielded variable results when assayed with coagulase test. Taken together, these data indicate the existence of extensive polymorphism at the coagulase gene locus in the genus Staphylococcus and exemplifies the extent of molecular and phenotypic heterogeneity associated with the strains circulating in rural herds. |
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Brazilian Journal of Microbiology |
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Detection of different Staphylococcus aureus strains in bovine milk from subclinical mastitis using PCR and routine techniquesbovine mastitisStaphylococcus aureuscoagulasePCRContamination of fresh milk with Staphylococcus aureus was assessed comparatively through routine phenotypic (coagulase tube test and coagulase slide test) and genotypic (PCR) screening of 128 S. aureus strains isolated from 555 milk samples. These samples were collected from 362 cows with subclinical mastitis, hosted in different dairy herds at various locations of the Northern and Northeastern rural areas of the State of Rio de Janeiro, 39.7% of which were CMT-positive. All S. aureus isolates tested positive for the presence of the coagulase gene by PCR and the isolates could be grouped into four distinct classes according to the size of the PCR product. The strains also yielded variable results when assayed with coagulase test. Taken together, these data indicate the existence of extensive polymorphism at the coagulase gene locus in the genus Staphylococcus and exemplifies the extent of molecular and phenotypic heterogeneity associated with the strains circulating in rural herds.Sociedade Brasileira de Microbiologia2001-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000100007Brazilian Journal of Microbiology v.32 n.1 2001reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822001000100007info:eu-repo/semantics/openAccessVieira-da-Motta,OlneyFolly,Márcio ManhãesSakyiama,Cássia Camargo Hageneng2001-08-02T00:00:00Zoai:scielo:S1517-83822001000100007Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2001-08-02T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false |
dc.title.none.fl_str_mv |
Detection of different Staphylococcus aureus strains in bovine milk from subclinical mastitis using PCR and routine techniques |
title |
Detection of different Staphylococcus aureus strains in bovine milk from subclinical mastitis using PCR and routine techniques |
spellingShingle |
Detection of different Staphylococcus aureus strains in bovine milk from subclinical mastitis using PCR and routine techniques Vieira-da-Motta,Olney bovine mastitis Staphylococcus aureus coagulase PCR |
title_short |
Detection of different Staphylococcus aureus strains in bovine milk from subclinical mastitis using PCR and routine techniques |
title_full |
Detection of different Staphylococcus aureus strains in bovine milk from subclinical mastitis using PCR and routine techniques |
title_fullStr |
Detection of different Staphylococcus aureus strains in bovine milk from subclinical mastitis using PCR and routine techniques |
title_full_unstemmed |
Detection of different Staphylococcus aureus strains in bovine milk from subclinical mastitis using PCR and routine techniques |
title_sort |
Detection of different Staphylococcus aureus strains in bovine milk from subclinical mastitis using PCR and routine techniques |
author |
Vieira-da-Motta,Olney |
author_facet |
Vieira-da-Motta,Olney Folly,Márcio Manhães Sakyiama,Cássia Camargo Hagen |
author_role |
author |
author2 |
Folly,Márcio Manhães Sakyiama,Cássia Camargo Hagen |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Vieira-da-Motta,Olney Folly,Márcio Manhães Sakyiama,Cássia Camargo Hagen |
dc.subject.por.fl_str_mv |
bovine mastitis Staphylococcus aureus coagulase PCR |
topic |
bovine mastitis Staphylococcus aureus coagulase PCR |
description |
Contamination of fresh milk with Staphylococcus aureus was assessed comparatively through routine phenotypic (coagulase tube test and coagulase slide test) and genotypic (PCR) screening of 128 S. aureus strains isolated from 555 milk samples. These samples were collected from 362 cows with subclinical mastitis, hosted in different dairy herds at various locations of the Northern and Northeastern rural areas of the State of Rio de Janeiro, 39.7% of which were CMT-positive. All S. aureus isolates tested positive for the presence of the coagulase gene by PCR and the isolates could be grouped into four distinct classes according to the size of the PCR product. The strains also yielded variable results when assayed with coagulase test. Taken together, these data indicate the existence of extensive polymorphism at the coagulase gene locus in the genus Staphylococcus and exemplifies the extent of molecular and phenotypic heterogeneity associated with the strains circulating in rural herds. |
publishDate |
2001 |
dc.date.none.fl_str_mv |
2001-03-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000100007 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000100007 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1517-83822001000100007 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
dc.source.none.fl_str_mv |
Brazilian Journal of Microbiology v.32 n.1 2001 reponame:Brazilian Journal of Microbiology instname:Sociedade Brasileira de Microbiologia (SBM) instacron:SBM |
instname_str |
Sociedade Brasileira de Microbiologia (SBM) |
instacron_str |
SBM |
institution |
SBM |
reponame_str |
Brazilian Journal of Microbiology |
collection |
Brazilian Journal of Microbiology |
repository.name.fl_str_mv |
Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM) |
repository.mail.fl_str_mv |
bjm@sbmicrobiologia.org.br||mbmartin@usp.br |
_version_ |
1752122198871506944 |