Transmission of Campylobacter coli in chicken embryos
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Microbiology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200014 |
Resumo: | Campylobacter coli is an important species involved in human cases of enteritis, and chickens are carriers of the pathogen mainly in developing country. The current study aimed to evaluate the transmission of C. coli and its pathogenic effects in chicken embryos. Breeder hens were inoculated intra-esophageally with C. coli isolated from chickens, and their eggs and embryos were analyzed for the presence of bacteria using real-time PCR and plate culture. The viability of embryos was verified. In parallel, SPF eggs were inoculated with C. coli in the air sac; after incubation, the embryos were submitted to the same analysis as the embryos from breeder hens. In embryos and fertile eggs from breeder hens, the bacterium was only identified by molecular methods; in the SPF eggs, however, the bacterium was detected by both techniques. The results showed no relationship between embryo mortality and positivity for C. coli in the embryos from breeder hens. However, the presence of bacteria is a cause of precocious mortality for SPF embryos. This study revealed that although the vertical transmission is a possible event, the bacteria can not grow in embryonic field samples. |
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Brazilian Journal of Microbiology |
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Transmission of Campylobacter coli in chicken embryosCampylobacter coliViabilityTransmissionBreeder hensCampylobacter coli is an important species involved in human cases of enteritis, and chickens are carriers of the pathogen mainly in developing country. The current study aimed to evaluate the transmission of C. coli and its pathogenic effects in chicken embryos. Breeder hens were inoculated intra-esophageally with C. coli isolated from chickens, and their eggs and embryos were analyzed for the presence of bacteria using real-time PCR and plate culture. The viability of embryos was verified. In parallel, SPF eggs were inoculated with C. coli in the air sac; after incubation, the embryos were submitted to the same analysis as the embryos from breeder hens. In embryos and fertile eggs from breeder hens, the bacterium was only identified by molecular methods; in the SPF eggs, however, the bacterium was detected by both techniques. The results showed no relationship between embryo mortality and positivity for C. coli in the embryos from breeder hens. However, the presence of bacteria is a cause of precocious mortality for SPF embryos. This study revealed that although the vertical transmission is a possible event, the bacteria can not grow in embryonic field samples.Sociedade Brasileira de Microbiologia2012-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200014Brazilian Journal of Microbiology v.43 n.2 2012reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822012000200014info:eu-repo/semantics/openAccessRossi,Daise AparecidaFonseca,Belchiolina BeatrizMelo,Roberta Torres deFelipe,Gutembergue da SilvaSilva,Paulo Lourenço daMendonça,Eliane PereiraFilgueiras,Ana Luzia LauriaBeletti,Marcelo Emilioeng2012-08-07T00:00:00Zoai:scielo:S1517-83822012000200014Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2012-08-07T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false |
dc.title.none.fl_str_mv |
Transmission of Campylobacter coli in chicken embryos |
title |
Transmission of Campylobacter coli in chicken embryos |
spellingShingle |
Transmission of Campylobacter coli in chicken embryos Rossi,Daise Aparecida Campylobacter coli Viability Transmission Breeder hens |
title_short |
Transmission of Campylobacter coli in chicken embryos |
title_full |
Transmission of Campylobacter coli in chicken embryos |
title_fullStr |
Transmission of Campylobacter coli in chicken embryos |
title_full_unstemmed |
Transmission of Campylobacter coli in chicken embryos |
title_sort |
Transmission of Campylobacter coli in chicken embryos |
author |
Rossi,Daise Aparecida |
author_facet |
Rossi,Daise Aparecida Fonseca,Belchiolina Beatriz Melo,Roberta Torres de Felipe,Gutembergue da Silva Silva,Paulo Lourenço da Mendonça,Eliane Pereira Filgueiras,Ana Luzia Lauria Beletti,Marcelo Emilio |
author_role |
author |
author2 |
Fonseca,Belchiolina Beatriz Melo,Roberta Torres de Felipe,Gutembergue da Silva Silva,Paulo Lourenço da Mendonça,Eliane Pereira Filgueiras,Ana Luzia Lauria Beletti,Marcelo Emilio |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Rossi,Daise Aparecida Fonseca,Belchiolina Beatriz Melo,Roberta Torres de Felipe,Gutembergue da Silva Silva,Paulo Lourenço da Mendonça,Eliane Pereira Filgueiras,Ana Luzia Lauria Beletti,Marcelo Emilio |
dc.subject.por.fl_str_mv |
Campylobacter coli Viability Transmission Breeder hens |
topic |
Campylobacter coli Viability Transmission Breeder hens |
description |
Campylobacter coli is an important species involved in human cases of enteritis, and chickens are carriers of the pathogen mainly in developing country. The current study aimed to evaluate the transmission of C. coli and its pathogenic effects in chicken embryos. Breeder hens were inoculated intra-esophageally with C. coli isolated from chickens, and their eggs and embryos were analyzed for the presence of bacteria using real-time PCR and plate culture. The viability of embryos was verified. In parallel, SPF eggs were inoculated with C. coli in the air sac; after incubation, the embryos were submitted to the same analysis as the embryos from breeder hens. In embryos and fertile eggs from breeder hens, the bacterium was only identified by molecular methods; in the SPF eggs, however, the bacterium was detected by both techniques. The results showed no relationship between embryo mortality and positivity for C. coli in the embryos from breeder hens. However, the presence of bacteria is a cause of precocious mortality for SPF embryos. This study revealed that although the vertical transmission is a possible event, the bacteria can not grow in embryonic field samples. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200014 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200014 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1517-83822012000200014 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
dc.source.none.fl_str_mv |
Brazilian Journal of Microbiology v.43 n.2 2012 reponame:Brazilian Journal of Microbiology instname:Sociedade Brasileira de Microbiologia (SBM) instacron:SBM |
instname_str |
Sociedade Brasileira de Microbiologia (SBM) |
instacron_str |
SBM |
institution |
SBM |
reponame_str |
Brazilian Journal of Microbiology |
collection |
Brazilian Journal of Microbiology |
repository.name.fl_str_mv |
Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM) |
repository.mail.fl_str_mv |
bjm@sbmicrobiologia.org.br||mbmartin@usp.br |
_version_ |
1752122204368142336 |