ERIC-PCR genotypic characterization of Haemophilus parasuis isolated from Brazilian swine
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Microbiology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400025 |
Resumo: | Haemophilus parasuis infection, known as Glässer's disease, is characterized by fibrinous polyserositis, arthritis and meningitis in piglets. Although traditional diagnosis is based on herd history, clinical signs, bacterial isolation and serotyping, the molecular-based methods are alternatives for species-specific tests and epidemiologic study. The aim of this study was to characterize H. parasuis strains isolated from different states of Brazil by serotyping, PCR and ERIC-PCR. Serotyping revealed serovar 4 as the most prevalent (24 %), followed by serovars 14 (14 %), 5 (12 %), 13 (8 %) and 2 (2 %), whereas 40 % of the strains were considered as non-typeable. From 50 strains tested 43 (86%) were positive to Group 1 vtaA gene that have been related to virulent strains of H.parasuis. ERIC-PCR was able to type isolates tested among 23 different patterns, including non-typeable strains. ERIC-PCR patterns were very heterogeneous and presented high similarity between strains of the same animal or farm origin. The results indicated ERIC-PCR as a valuable tool for typing H. parasuis isolates collected in Brazil. |
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Brazilian Journal of Microbiology |
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ERIC-PCR genotypic characterization of Haemophilus parasuis isolated from Brazilian swineHaemophilus parasuisGlässer diseaseGenotypingERIC-PCRHaemophilus parasuis infection, known as Glässer's disease, is characterized by fibrinous polyserositis, arthritis and meningitis in piglets. Although traditional diagnosis is based on herd history, clinical signs, bacterial isolation and serotyping, the molecular-based methods are alternatives for species-specific tests and epidemiologic study. The aim of this study was to characterize H. parasuis strains isolated from different states of Brazil by serotyping, PCR and ERIC-PCR. Serotyping revealed serovar 4 as the most prevalent (24 %), followed by serovars 14 (14 %), 5 (12 %), 13 (8 %) and 2 (2 %), whereas 40 % of the strains were considered as non-typeable. From 50 strains tested 43 (86%) were positive to Group 1 vtaA gene that have been related to virulent strains of H.parasuis. ERIC-PCR was able to type isolates tested among 23 different patterns, including non-typeable strains. ERIC-PCR patterns were very heterogeneous and presented high similarity between strains of the same animal or farm origin. The results indicated ERIC-PCR as a valuable tool for typing H. parasuis isolates collected in Brazil.Sociedade Brasileira de Microbiologia2011-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400025Brazilian Journal of Microbiology v.42 n.4 2011reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822011000400025info:eu-repo/semantics/openAccessMoreno,Luisa ZanolliCastilla,Karina SalvagniGobbi,Débora Dirani Sena deCoutinho,Tania AlenFerreira,Thais Sebastiana PorfidaMoreno,Andrea Mickeeng2015-06-30T00:00:00Zoai:scielo:S1517-83822011000400025Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2015-06-30T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false |
dc.title.none.fl_str_mv |
ERIC-PCR genotypic characterization of Haemophilus parasuis isolated from Brazilian swine |
title |
ERIC-PCR genotypic characterization of Haemophilus parasuis isolated from Brazilian swine |
spellingShingle |
ERIC-PCR genotypic characterization of Haemophilus parasuis isolated from Brazilian swine Moreno,Luisa Zanolli Haemophilus parasuis Glässer disease Genotyping ERIC-PCR |
title_short |
ERIC-PCR genotypic characterization of Haemophilus parasuis isolated from Brazilian swine |
title_full |
ERIC-PCR genotypic characterization of Haemophilus parasuis isolated from Brazilian swine |
title_fullStr |
ERIC-PCR genotypic characterization of Haemophilus parasuis isolated from Brazilian swine |
title_full_unstemmed |
ERIC-PCR genotypic characterization of Haemophilus parasuis isolated from Brazilian swine |
title_sort |
ERIC-PCR genotypic characterization of Haemophilus parasuis isolated from Brazilian swine |
author |
Moreno,Luisa Zanolli |
author_facet |
Moreno,Luisa Zanolli Castilla,Karina Salvagni Gobbi,Débora Dirani Sena de Coutinho,Tania Alen Ferreira,Thais Sebastiana Porfida Moreno,Andrea Micke |
author_role |
author |
author2 |
Castilla,Karina Salvagni Gobbi,Débora Dirani Sena de Coutinho,Tania Alen Ferreira,Thais Sebastiana Porfida Moreno,Andrea Micke |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Moreno,Luisa Zanolli Castilla,Karina Salvagni Gobbi,Débora Dirani Sena de Coutinho,Tania Alen Ferreira,Thais Sebastiana Porfida Moreno,Andrea Micke |
dc.subject.por.fl_str_mv |
Haemophilus parasuis Glässer disease Genotyping ERIC-PCR |
topic |
Haemophilus parasuis Glässer disease Genotyping ERIC-PCR |
description |
Haemophilus parasuis infection, known as Glässer's disease, is characterized by fibrinous polyserositis, arthritis and meningitis in piglets. Although traditional diagnosis is based on herd history, clinical signs, bacterial isolation and serotyping, the molecular-based methods are alternatives for species-specific tests and epidemiologic study. The aim of this study was to characterize H. parasuis strains isolated from different states of Brazil by serotyping, PCR and ERIC-PCR. Serotyping revealed serovar 4 as the most prevalent (24 %), followed by serovars 14 (14 %), 5 (12 %), 13 (8 %) and 2 (2 %), whereas 40 % of the strains were considered as non-typeable. From 50 strains tested 43 (86%) were positive to Group 1 vtaA gene that have been related to virulent strains of H.parasuis. ERIC-PCR was able to type isolates tested among 23 different patterns, including non-typeable strains. ERIC-PCR patterns were very heterogeneous and presented high similarity between strains of the same animal or farm origin. The results indicated ERIC-PCR as a valuable tool for typing H. parasuis isolates collected in Brazil. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400025 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400025 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1517-83822011000400025 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
dc.source.none.fl_str_mv |
Brazilian Journal of Microbiology v.42 n.4 2011 reponame:Brazilian Journal of Microbiology instname:Sociedade Brasileira de Microbiologia (SBM) instacron:SBM |
instname_str |
Sociedade Brasileira de Microbiologia (SBM) |
instacron_str |
SBM |
institution |
SBM |
reponame_str |
Brazilian Journal of Microbiology |
collection |
Brazilian Journal of Microbiology |
repository.name.fl_str_mv |
Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM) |
repository.mail.fl_str_mv |
bjm@sbmicrobiologia.org.br||mbmartin@usp.br |
_version_ |
1752122203979120640 |