Detection of leptospires in clinically healthy piglets born from sows experimentally infected with Leptospira interrogans serovar Canicola
Autor(a) principal: | |
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Data de Publicação: | 2006 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Microbiology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000400034 |
Resumo: | Leptospirosis is an important zoonosis that causes reproductive disorders in swine. The isolation of leptospires from aborted fetuses, stillbirths and weak piglets was obtained in several occasions, however, the bacteria was never isolated from apparently healthy piglets born from apparently healthy infected dams. Six sows of the Landrace breed with a known date of service and pregnancy confirmed by ultrasonography were infected intravenously with 5ml of Leptospira interrogans serovar Canicola inoculum between 76 and 90 days of gestation, and one week after farrowing, at least one piglet per sow was euthanized and samples of liver, kidneys, lungs, heart, spleen and gastric content were taken and examined by PCR. Reproductive disorders or any clinical sign of infection were not observed in the inoculated sows. The piglets born from these animals presented no clinical signs or macroscopic lesions that could be attributed to leptospirosis. All inoculated sows presented anti-leptospires antibodies by microscopic serum-agglutination test (MAT) in the postinoculation serum samples and leptospires were not found in the urine as well as were not detected by PCR applied in this material, however, PCR accomplished in kidneys and liver from a euthanized sow presented positive results. Of the total of 12 euthanized piglets, 10 (83.3%) presented positive results by PCR in at least one of the kidney, liver, heart, spleen, lung and gastric content samples. The present study reports the vertical transmission of the infection and the detection of leptospires in clinically healthy piglets born from experimentally infected sows, which is important of the epidemiological point of view as the maintenance of clinically healthy infected animals may allow the persistence of the bacteria in the herd, exposing other animals to the risk of infection. |
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Brazilian Journal of Microbiology |
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Detection of leptospires in clinically healthy piglets born from sows experimentally infected with Leptospira interrogans serovar CanicolaExperimental swine leptospirosisLeptospira spp.serovar Canicolavertical transmissionLeptospirosis is an important zoonosis that causes reproductive disorders in swine. The isolation of leptospires from aborted fetuses, stillbirths and weak piglets was obtained in several occasions, however, the bacteria was never isolated from apparently healthy piglets born from apparently healthy infected dams. Six sows of the Landrace breed with a known date of service and pregnancy confirmed by ultrasonography were infected intravenously with 5ml of Leptospira interrogans serovar Canicola inoculum between 76 and 90 days of gestation, and one week after farrowing, at least one piglet per sow was euthanized and samples of liver, kidneys, lungs, heart, spleen and gastric content were taken and examined by PCR. Reproductive disorders or any clinical sign of infection were not observed in the inoculated sows. The piglets born from these animals presented no clinical signs or macroscopic lesions that could be attributed to leptospirosis. All inoculated sows presented anti-leptospires antibodies by microscopic serum-agglutination test (MAT) in the postinoculation serum samples and leptospires were not found in the urine as well as were not detected by PCR applied in this material, however, PCR accomplished in kidneys and liver from a euthanized sow presented positive results. Of the total of 12 euthanized piglets, 10 (83.3%) presented positive results by PCR in at least one of the kidney, liver, heart, spleen, lung and gastric content samples. The present study reports the vertical transmission of the infection and the detection of leptospires in clinically healthy piglets born from experimentally infected sows, which is important of the epidemiological point of view as the maintenance of clinically healthy infected animals may allow the persistence of the bacteria in the herd, exposing other animals to the risk of infection.Sociedade Brasileira de Microbiologia2006-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000400034Brazilian Journal of Microbiology v.37 n.4 2006reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822006000400034info:eu-repo/semantics/openAccessSoto,Francisco Rafael M.Azevedo,Sérgio Santos deMorais,Zenaide Maria dePinheiro,Sônia ReginaDelbem,Ádina Cléia B.Moreno,Andréa MickePaixão,RenataVuaden,Erlete R.Vasconcellos,Sílvio Arrudaeng2007-03-01T00:00:00Zoai:scielo:S1517-83822006000400034Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2007-03-01T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false |
dc.title.none.fl_str_mv |
Detection of leptospires in clinically healthy piglets born from sows experimentally infected with Leptospira interrogans serovar Canicola |
title |
Detection of leptospires in clinically healthy piglets born from sows experimentally infected with Leptospira interrogans serovar Canicola |
spellingShingle |
Detection of leptospires in clinically healthy piglets born from sows experimentally infected with Leptospira interrogans serovar Canicola Soto,Francisco Rafael M. Experimental swine leptospirosis Leptospira spp. serovar Canicola vertical transmission |
title_short |
Detection of leptospires in clinically healthy piglets born from sows experimentally infected with Leptospira interrogans serovar Canicola |
title_full |
Detection of leptospires in clinically healthy piglets born from sows experimentally infected with Leptospira interrogans serovar Canicola |
title_fullStr |
Detection of leptospires in clinically healthy piglets born from sows experimentally infected with Leptospira interrogans serovar Canicola |
title_full_unstemmed |
Detection of leptospires in clinically healthy piglets born from sows experimentally infected with Leptospira interrogans serovar Canicola |
title_sort |
Detection of leptospires in clinically healthy piglets born from sows experimentally infected with Leptospira interrogans serovar Canicola |
author |
Soto,Francisco Rafael M. |
author_facet |
Soto,Francisco Rafael M. Azevedo,Sérgio Santos de Morais,Zenaide Maria de Pinheiro,Sônia Regina Delbem,Ádina Cléia B. Moreno,Andréa Micke Paixão,Renata Vuaden,Erlete R. Vasconcellos,Sílvio Arruda |
author_role |
author |
author2 |
Azevedo,Sérgio Santos de Morais,Zenaide Maria de Pinheiro,Sônia Regina Delbem,Ádina Cléia B. Moreno,Andréa Micke Paixão,Renata Vuaden,Erlete R. Vasconcellos,Sílvio Arruda |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Soto,Francisco Rafael M. Azevedo,Sérgio Santos de Morais,Zenaide Maria de Pinheiro,Sônia Regina Delbem,Ádina Cléia B. Moreno,Andréa Micke Paixão,Renata Vuaden,Erlete R. Vasconcellos,Sílvio Arruda |
dc.subject.por.fl_str_mv |
Experimental swine leptospirosis Leptospira spp. serovar Canicola vertical transmission |
topic |
Experimental swine leptospirosis Leptospira spp. serovar Canicola vertical transmission |
description |
Leptospirosis is an important zoonosis that causes reproductive disorders in swine. The isolation of leptospires from aborted fetuses, stillbirths and weak piglets was obtained in several occasions, however, the bacteria was never isolated from apparently healthy piglets born from apparently healthy infected dams. Six sows of the Landrace breed with a known date of service and pregnancy confirmed by ultrasonography were infected intravenously with 5ml of Leptospira interrogans serovar Canicola inoculum between 76 and 90 days of gestation, and one week after farrowing, at least one piglet per sow was euthanized and samples of liver, kidneys, lungs, heart, spleen and gastric content were taken and examined by PCR. Reproductive disorders or any clinical sign of infection were not observed in the inoculated sows. The piglets born from these animals presented no clinical signs or macroscopic lesions that could be attributed to leptospirosis. All inoculated sows presented anti-leptospires antibodies by microscopic serum-agglutination test (MAT) in the postinoculation serum samples and leptospires were not found in the urine as well as were not detected by PCR applied in this material, however, PCR accomplished in kidneys and liver from a euthanized sow presented positive results. Of the total of 12 euthanized piglets, 10 (83.3%) presented positive results by PCR in at least one of the kidney, liver, heart, spleen, lung and gastric content samples. The present study reports the vertical transmission of the infection and the detection of leptospires in clinically healthy piglets born from experimentally infected sows, which is important of the epidemiological point of view as the maintenance of clinically healthy infected animals may allow the persistence of the bacteria in the herd, exposing other animals to the risk of infection. |
publishDate |
2006 |
dc.date.none.fl_str_mv |
2006-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000400034 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000400034 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1517-83822006000400034 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
dc.source.none.fl_str_mv |
Brazilian Journal of Microbiology v.37 n.4 2006 reponame:Brazilian Journal of Microbiology instname:Sociedade Brasileira de Microbiologia (SBM) instacron:SBM |
instname_str |
Sociedade Brasileira de Microbiologia (SBM) |
instacron_str |
SBM |
institution |
SBM |
reponame_str |
Brazilian Journal of Microbiology |
collection |
Brazilian Journal of Microbiology |
repository.name.fl_str_mv |
Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM) |
repository.mail.fl_str_mv |
bjm@sbmicrobiologia.org.br||mbmartin@usp.br |
_version_ |
1752122200942444544 |