A simple and rapid method of sample preparation from culture filtrate of M. tuberculosis for two-dimensional gel electrophoresis

Bibliographic Details
Main Author: Kumar,Gavish
Publication Date: 2010
Other Authors: Shankar,Hari, Bisht,Deepa, Sharma,Prashant, Singhal,Neelja, Katoch,V.M., Joshi,Beenu
Format: Article
Language: eng
Source: Brazilian Journal of Microbiology
Download full: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000200005
Summary: Sample preparation for Two-dimensional gel electrophoresis (2DE) is tedious and not sufficient to provide a comparative profile of secreted proteins for various strains of M. tuberculosis. High lipid content in mycobacteria limits the use of common methods as it can hinder the 2DE run. This study highlights the significance of SDS-TCA procedure over common used methods for the preparation of sample from culture filtrate as well as other proteinaceous fluids.
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spelling A simple and rapid method of sample preparation from culture filtrate of M. tuberculosis for two-dimensional gel electrophoresisCulture filtrate proteinsM. tuberculosisSDS-TCA procedureTwo-dimensional gel electrophoresisSample preparation for Two-dimensional gel electrophoresis (2DE) is tedious and not sufficient to provide a comparative profile of secreted proteins for various strains of M. tuberculosis. High lipid content in mycobacteria limits the use of common methods as it can hinder the 2DE run. This study highlights the significance of SDS-TCA procedure over common used methods for the preparation of sample from culture filtrate as well as other proteinaceous fluids.Sociedade Brasileira de Microbiologia2010-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000200005Brazilian Journal of Microbiology v.41 n.2 2010reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822010000200005info:eu-repo/semantics/openAccessKumar,GavishShankar,HariBisht,DeepaSharma,PrashantSinghal,NeeljaKatoch,V.M.Joshi,Beenueng2010-04-16T00:00:00Zoai:scielo:S1517-83822010000200005Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2010-04-16T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false
dc.title.none.fl_str_mv A simple and rapid method of sample preparation from culture filtrate of M. tuberculosis for two-dimensional gel electrophoresis
title A simple and rapid method of sample preparation from culture filtrate of M. tuberculosis for two-dimensional gel electrophoresis
spellingShingle A simple and rapid method of sample preparation from culture filtrate of M. tuberculosis for two-dimensional gel electrophoresis
Kumar,Gavish
Culture filtrate proteins
M. tuberculosis
SDS-TCA procedure
Two-dimensional gel electrophoresis
title_short A simple and rapid method of sample preparation from culture filtrate of M. tuberculosis for two-dimensional gel electrophoresis
title_full A simple and rapid method of sample preparation from culture filtrate of M. tuberculosis for two-dimensional gel electrophoresis
title_fullStr A simple and rapid method of sample preparation from culture filtrate of M. tuberculosis for two-dimensional gel electrophoresis
title_full_unstemmed A simple and rapid method of sample preparation from culture filtrate of M. tuberculosis for two-dimensional gel electrophoresis
title_sort A simple and rapid method of sample preparation from culture filtrate of M. tuberculosis for two-dimensional gel electrophoresis
author Kumar,Gavish
author_facet Kumar,Gavish
Shankar,Hari
Bisht,Deepa
Sharma,Prashant
Singhal,Neelja
Katoch,V.M.
Joshi,Beenu
author_role author
author2 Shankar,Hari
Bisht,Deepa
Sharma,Prashant
Singhal,Neelja
Katoch,V.M.
Joshi,Beenu
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Kumar,Gavish
Shankar,Hari
Bisht,Deepa
Sharma,Prashant
Singhal,Neelja
Katoch,V.M.
Joshi,Beenu
dc.subject.por.fl_str_mv Culture filtrate proteins
M. tuberculosis
SDS-TCA procedure
Two-dimensional gel electrophoresis
topic Culture filtrate proteins
M. tuberculosis
SDS-TCA procedure
Two-dimensional gel electrophoresis
description Sample preparation for Two-dimensional gel electrophoresis (2DE) is tedious and not sufficient to provide a comparative profile of secreted proteins for various strains of M. tuberculosis. High lipid content in mycobacteria limits the use of common methods as it can hinder the 2DE run. This study highlights the significance of SDS-TCA procedure over common used methods for the preparation of sample from culture filtrate as well as other proteinaceous fluids.
publishDate 2010
dc.date.none.fl_str_mv 2010-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000200005
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000200005
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1517-83822010000200005
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv Brazilian Journal of Microbiology v.41 n.2 2010
reponame:Brazilian Journal of Microbiology
instname:Sociedade Brasileira de Microbiologia (SBM)
instacron:SBM
instname_str Sociedade Brasileira de Microbiologia (SBM)
instacron_str SBM
institution SBM
reponame_str Brazilian Journal of Microbiology
collection Brazilian Journal of Microbiology
repository.name.fl_str_mv Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)
repository.mail.fl_str_mv bjm@sbmicrobiologia.org.br||mbmartin@usp.br
_version_ 1752122202737606656

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