Detection of Mycobacterium in clinical samples by multiprimer polymerase chain reaction

Detalhes bibliográficos
Autor(a) principal: Barouni,Abdulmonam Saied
Data de Publicação: 2004
Outros Autores: Saridakis,Halha Ostrensky, Vidotto,Marilda Carlos
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Microbiology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000100004
Resumo: We investigated the use of multiprimer-PCR for detection of mycobacteria species in clinical samples. Three different mycobacterial genomic fragments were investigated: the IS6110 insertion sequence, present in M. tuberculosis complex; the genus specific fragment (32kDa); and from M. tuberculosis species-specific mtp40 gene. The sensitivity and specificity using 135 clinical isolates were 94.5% and 95.9%, respectively, compared with culture in Löwenstein-Jensen medium; the detection limit was 0.05ng of DNA. In conclusion, this assay is reliable and rapid for detection of Mycobacterium species in clinical samples, and differentiates M. tuberculosis from M. bovis strains in a single-step assay.
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spelling Detection of Mycobacterium in clinical samples by multiprimer polymerase chain reactionMycobacterium tuberculosismultiprimer-PCRmolecular diagnosisWe investigated the use of multiprimer-PCR for detection of mycobacteria species in clinical samples. Three different mycobacterial genomic fragments were investigated: the IS6110 insertion sequence, present in M. tuberculosis complex; the genus specific fragment (32kDa); and from M. tuberculosis species-specific mtp40 gene. The sensitivity and specificity using 135 clinical isolates were 94.5% and 95.9%, respectively, compared with culture in Löwenstein-Jensen medium; the detection limit was 0.05ng of DNA. In conclusion, this assay is reliable and rapid for detection of Mycobacterium species in clinical samples, and differentiates M. tuberculosis from M. bovis strains in a single-step assay.Sociedade Brasileira de Microbiologia2004-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000100004Brazilian Journal of Microbiology v.35 n.1-2 2004reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822004000100004info:eu-repo/semantics/openAccessBarouni,Abdulmonam SaiedSaridakis,Halha OstrenskyVidotto,Marilda Carloseng2004-11-16T00:00:00Zoai:scielo:S1517-83822004000100004Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2004-11-16T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false
dc.title.none.fl_str_mv Detection of Mycobacterium in clinical samples by multiprimer polymerase chain reaction
title Detection of Mycobacterium in clinical samples by multiprimer polymerase chain reaction
spellingShingle Detection of Mycobacterium in clinical samples by multiprimer polymerase chain reaction
Barouni,Abdulmonam Saied
Mycobacterium tuberculosis
multiprimer-PCR
molecular diagnosis
title_short Detection of Mycobacterium in clinical samples by multiprimer polymerase chain reaction
title_full Detection of Mycobacterium in clinical samples by multiprimer polymerase chain reaction
title_fullStr Detection of Mycobacterium in clinical samples by multiprimer polymerase chain reaction
title_full_unstemmed Detection of Mycobacterium in clinical samples by multiprimer polymerase chain reaction
title_sort Detection of Mycobacterium in clinical samples by multiprimer polymerase chain reaction
author Barouni,Abdulmonam Saied
author_facet Barouni,Abdulmonam Saied
Saridakis,Halha Ostrensky
Vidotto,Marilda Carlos
author_role author
author2 Saridakis,Halha Ostrensky
Vidotto,Marilda Carlos
author2_role author
author
dc.contributor.author.fl_str_mv Barouni,Abdulmonam Saied
Saridakis,Halha Ostrensky
Vidotto,Marilda Carlos
dc.subject.por.fl_str_mv Mycobacterium tuberculosis
multiprimer-PCR
molecular diagnosis
topic Mycobacterium tuberculosis
multiprimer-PCR
molecular diagnosis
description We investigated the use of multiprimer-PCR for detection of mycobacteria species in clinical samples. Three different mycobacterial genomic fragments were investigated: the IS6110 insertion sequence, present in M. tuberculosis complex; the genus specific fragment (32kDa); and from M. tuberculosis species-specific mtp40 gene. The sensitivity and specificity using 135 clinical isolates were 94.5% and 95.9%, respectively, compared with culture in Löwenstein-Jensen medium; the detection limit was 0.05ng of DNA. In conclusion, this assay is reliable and rapid for detection of Mycobacterium species in clinical samples, and differentiates M. tuberculosis from M. bovis strains in a single-step assay.
publishDate 2004
dc.date.none.fl_str_mv 2004-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000100004
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000100004
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1517-83822004000100004
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv Brazilian Journal of Microbiology v.35 n.1-2 2004
reponame:Brazilian Journal of Microbiology
instname:Sociedade Brasileira de Microbiologia (SBM)
instacron:SBM
instname_str Sociedade Brasileira de Microbiologia (SBM)
instacron_str SBM
institution SBM
reponame_str Brazilian Journal of Microbiology
collection Brazilian Journal of Microbiology
repository.name.fl_str_mv Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)
repository.mail.fl_str_mv bjm@sbmicrobiologia.org.br||mbmartin@usp.br
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