Thin layer microcolony culture associated with PCR for early identification of Mycobacterium bovis

Detalhes bibliográficos
Autor(a) principal: Rosário,Tatiana Reis do
Data de Publicação: 2014
Outros Autores: Dib,Cristina Corsi, Roxo,Eliana, Pinheiro,Sônia Regina, Vasconcellos,Silvio Arruda, Benites,Nilson Roberti
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Microbiology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100031
Resumo: The initial growth of mycobacteria from 49 samples of cattle and buffalo organs collected in commercial slaughterhouses was compared between modified Middlebrook 7H11 thin layer microcolony culture and Stonebrink medium used in the isolation of Mycobacterium bovis. Aliquots were decontaminated by Petroff's method, processed and cultured in both media. The identity of the acid-fast bacilli stained by Ziehl-Neelsen was confirmed by PCR. Optical microscopy showed that results of the early observation of Mycobacterium bovis colonies in thin layer culture were similar to those obtained in macroscopic observation of the colonies in Stonebrink medium. However, early observation of the colonies enabled early confirmation by PCR, given the shorter time to the visualization of colonies when thin layer culture was used (between the 12nd and 25th day of culture).
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spelling Thin layer microcolony culture associated with PCR for early identification of Mycobacterium bovistuberculosisMycobacterium bovisthin layerPCRcattle and buffalo organsThe initial growth of mycobacteria from 49 samples of cattle and buffalo organs collected in commercial slaughterhouses was compared between modified Middlebrook 7H11 thin layer microcolony culture and Stonebrink medium used in the isolation of Mycobacterium bovis. Aliquots were decontaminated by Petroff's method, processed and cultured in both media. The identity of the acid-fast bacilli stained by Ziehl-Neelsen was confirmed by PCR. Optical microscopy showed that results of the early observation of Mycobacterium bovis colonies in thin layer culture were similar to those obtained in macroscopic observation of the colonies in Stonebrink medium. However, early observation of the colonies enabled early confirmation by PCR, given the shorter time to the visualization of colonies when thin layer culture was used (between the 12nd and 25th day of culture).Sociedade Brasileira de Microbiologia2014-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100031Brazilian Journal of Microbiology v.45 n.1 2014reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822014005000038info:eu-repo/semantics/openAccessRosário,Tatiana Reis doDib,Cristina CorsiRoxo,ElianaPinheiro,Sônia ReginaVasconcellos,Silvio ArrudaBenites,Nilson Robertieng2014-05-29T00:00:00Zoai:scielo:S1517-83822014000100031Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2014-05-29T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false
dc.title.none.fl_str_mv Thin layer microcolony culture associated with PCR for early identification of Mycobacterium bovis
title Thin layer microcolony culture associated with PCR for early identification of Mycobacterium bovis
spellingShingle Thin layer microcolony culture associated with PCR for early identification of Mycobacterium bovis
Rosário,Tatiana Reis do
tuberculosis
Mycobacterium bovis
thin layer
PCR
cattle and buffalo organs
title_short Thin layer microcolony culture associated with PCR for early identification of Mycobacterium bovis
title_full Thin layer microcolony culture associated with PCR for early identification of Mycobacterium bovis
title_fullStr Thin layer microcolony culture associated with PCR for early identification of Mycobacterium bovis
title_full_unstemmed Thin layer microcolony culture associated with PCR for early identification of Mycobacterium bovis
title_sort Thin layer microcolony culture associated with PCR for early identification of Mycobacterium bovis
author Rosário,Tatiana Reis do
author_facet Rosário,Tatiana Reis do
Dib,Cristina Corsi
Roxo,Eliana
Pinheiro,Sônia Regina
Vasconcellos,Silvio Arruda
Benites,Nilson Roberti
author_role author
author2 Dib,Cristina Corsi
Roxo,Eliana
Pinheiro,Sônia Regina
Vasconcellos,Silvio Arruda
Benites,Nilson Roberti
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Rosário,Tatiana Reis do
Dib,Cristina Corsi
Roxo,Eliana
Pinheiro,Sônia Regina
Vasconcellos,Silvio Arruda
Benites,Nilson Roberti
dc.subject.por.fl_str_mv tuberculosis
Mycobacterium bovis
thin layer
PCR
cattle and buffalo organs
topic tuberculosis
Mycobacterium bovis
thin layer
PCR
cattle and buffalo organs
description The initial growth of mycobacteria from 49 samples of cattle and buffalo organs collected in commercial slaughterhouses was compared between modified Middlebrook 7H11 thin layer microcolony culture and Stonebrink medium used in the isolation of Mycobacterium bovis. Aliquots were decontaminated by Petroff's method, processed and cultured in both media. The identity of the acid-fast bacilli stained by Ziehl-Neelsen was confirmed by PCR. Optical microscopy showed that results of the early observation of Mycobacterium bovis colonies in thin layer culture were similar to those obtained in macroscopic observation of the colonies in Stonebrink medium. However, early observation of the colonies enabled early confirmation by PCR, given the shorter time to the visualization of colonies when thin layer culture was used (between the 12nd and 25th day of culture).
publishDate 2014
dc.date.none.fl_str_mv 2014-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100031
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100031
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1517-83822014005000038
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv Brazilian Journal of Microbiology v.45 n.1 2014
reponame:Brazilian Journal of Microbiology
instname:Sociedade Brasileira de Microbiologia (SBM)
instacron:SBM
instname_str Sociedade Brasileira de Microbiologia (SBM)
instacron_str SBM
institution SBM
reponame_str Brazilian Journal of Microbiology
collection Brazilian Journal of Microbiology
repository.name.fl_str_mv Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)
repository.mail.fl_str_mv bjm@sbmicrobiologia.org.br||mbmartin@usp.br
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