Production of 11alpha-hydroxyprogesterone using Aspergillus terreus immobilized on polytetrafluoroethylene

Detalhes bibliográficos
Autor(a) principal: Ahmed,E.M.
Data de Publicação: 2007
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Microbiology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000200007
Resumo: In attempt to immobilize the mycelial fungi on a new hydrophobic matrix, Aspergillus terreus mycelia were immobilized into polytetrafluoroethylen (PTFE) and used for selected conversion of progesterone to 11alpha-hydroxyprogesterone. The immobilized cells exhibited higher yields than the free cells. Different parameters that can affect the cell activity were measured. The optimum pH was 6 and the optimum temperature was 30ºC. Higher transformation activities were achieved with matrix contained 40% PTFE. No considerable loss in yield was observed with storage of the cells at 4ºC for 30 days. Utilization of the immobilized cells in repeated batch processes indicated that the cells retained about 84% of their activity after reuse for eight successive cycles.
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spelling Production of 11alpha-hydroxyprogesterone using Aspergillus terreus immobilized on polytetrafluoroethylene11alpha-hydroxprogesteronepolytetrafluoroethyleneAspergillus terreusIn attempt to immobilize the mycelial fungi on a new hydrophobic matrix, Aspergillus terreus mycelia were immobilized into polytetrafluoroethylen (PTFE) and used for selected conversion of progesterone to 11alpha-hydroxyprogesterone. The immobilized cells exhibited higher yields than the free cells. Different parameters that can affect the cell activity were measured. The optimum pH was 6 and the optimum temperature was 30ºC. Higher transformation activities were achieved with matrix contained 40% PTFE. No considerable loss in yield was observed with storage of the cells at 4ºC for 30 days. Utilization of the immobilized cells in repeated batch processes indicated that the cells retained about 84% of their activity after reuse for eight successive cycles.Sociedade Brasileira de Microbiologia2007-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000200007Brazilian Journal of Microbiology v.38 n.2 2007reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822007000200007info:eu-repo/semantics/openAccessAhmed,E.M.eng2007-06-29T00:00:00Zoai:scielo:S1517-83822007000200007Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2007-06-29T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false
dc.title.none.fl_str_mv Production of 11alpha-hydroxyprogesterone using Aspergillus terreus immobilized on polytetrafluoroethylene
title Production of 11alpha-hydroxyprogesterone using Aspergillus terreus immobilized on polytetrafluoroethylene
spellingShingle Production of 11alpha-hydroxyprogesterone using Aspergillus terreus immobilized on polytetrafluoroethylene
Ahmed,E.M.
11alpha-hydroxprogesterone
polytetrafluoroethylene
Aspergillus terreus
title_short Production of 11alpha-hydroxyprogesterone using Aspergillus terreus immobilized on polytetrafluoroethylene
title_full Production of 11alpha-hydroxyprogesterone using Aspergillus terreus immobilized on polytetrafluoroethylene
title_fullStr Production of 11alpha-hydroxyprogesterone using Aspergillus terreus immobilized on polytetrafluoroethylene
title_full_unstemmed Production of 11alpha-hydroxyprogesterone using Aspergillus terreus immobilized on polytetrafluoroethylene
title_sort Production of 11alpha-hydroxyprogesterone using Aspergillus terreus immobilized on polytetrafluoroethylene
author Ahmed,E.M.
author_facet Ahmed,E.M.
author_role author
dc.contributor.author.fl_str_mv Ahmed,E.M.
dc.subject.por.fl_str_mv 11alpha-hydroxprogesterone
polytetrafluoroethylene
Aspergillus terreus
topic 11alpha-hydroxprogesterone
polytetrafluoroethylene
Aspergillus terreus
description In attempt to immobilize the mycelial fungi on a new hydrophobic matrix, Aspergillus terreus mycelia were immobilized into polytetrafluoroethylen (PTFE) and used for selected conversion of progesterone to 11alpha-hydroxyprogesterone. The immobilized cells exhibited higher yields than the free cells. Different parameters that can affect the cell activity were measured. The optimum pH was 6 and the optimum temperature was 30ºC. Higher transformation activities were achieved with matrix contained 40% PTFE. No considerable loss in yield was observed with storage of the cells at 4ºC for 30 days. Utilization of the immobilized cells in repeated batch processes indicated that the cells retained about 84% of their activity after reuse for eight successive cycles.
publishDate 2007
dc.date.none.fl_str_mv 2007-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000200007
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000200007
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1517-83822007000200007
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv Brazilian Journal of Microbiology v.38 n.2 2007
reponame:Brazilian Journal of Microbiology
instname:Sociedade Brasileira de Microbiologia (SBM)
instacron:SBM
instname_str Sociedade Brasileira de Microbiologia (SBM)
instacron_str SBM
institution SBM
reponame_str Brazilian Journal of Microbiology
collection Brazilian Journal of Microbiology
repository.name.fl_str_mv Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)
repository.mail.fl_str_mv bjm@sbmicrobiologia.org.br||mbmartin@usp.br
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