Production of 11alpha-hydroxyprogesterone using Aspergillus terreus immobilized on polytetrafluoroethylene
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Microbiology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000200007 |
Resumo: | In attempt to immobilize the mycelial fungi on a new hydrophobic matrix, Aspergillus terreus mycelia were immobilized into polytetrafluoroethylen (PTFE) and used for selected conversion of progesterone to 11alpha-hydroxyprogesterone. The immobilized cells exhibited higher yields than the free cells. Different parameters that can affect the cell activity were measured. The optimum pH was 6 and the optimum temperature was 30ºC. Higher transformation activities were achieved with matrix contained 40% PTFE. No considerable loss in yield was observed with storage of the cells at 4ºC for 30 days. Utilization of the immobilized cells in repeated batch processes indicated that the cells retained about 84% of their activity after reuse for eight successive cycles. |
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Brazilian Journal of Microbiology |
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Production of 11alpha-hydroxyprogesterone using Aspergillus terreus immobilized on polytetrafluoroethylene11alpha-hydroxprogesteronepolytetrafluoroethyleneAspergillus terreusIn attempt to immobilize the mycelial fungi on a new hydrophobic matrix, Aspergillus terreus mycelia were immobilized into polytetrafluoroethylen (PTFE) and used for selected conversion of progesterone to 11alpha-hydroxyprogesterone. The immobilized cells exhibited higher yields than the free cells. Different parameters that can affect the cell activity were measured. The optimum pH was 6 and the optimum temperature was 30ºC. Higher transformation activities were achieved with matrix contained 40% PTFE. No considerable loss in yield was observed with storage of the cells at 4ºC for 30 days. Utilization of the immobilized cells in repeated batch processes indicated that the cells retained about 84% of their activity after reuse for eight successive cycles.Sociedade Brasileira de Microbiologia2007-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000200007Brazilian Journal of Microbiology v.38 n.2 2007reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822007000200007info:eu-repo/semantics/openAccessAhmed,E.M.eng2007-06-29T00:00:00Zoai:scielo:S1517-83822007000200007Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2007-06-29T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false |
dc.title.none.fl_str_mv |
Production of 11alpha-hydroxyprogesterone using Aspergillus terreus immobilized on polytetrafluoroethylene |
title |
Production of 11alpha-hydroxyprogesterone using Aspergillus terreus immobilized on polytetrafluoroethylene |
spellingShingle |
Production of 11alpha-hydroxyprogesterone using Aspergillus terreus immobilized on polytetrafluoroethylene Ahmed,E.M. 11alpha-hydroxprogesterone polytetrafluoroethylene Aspergillus terreus |
title_short |
Production of 11alpha-hydroxyprogesterone using Aspergillus terreus immobilized on polytetrafluoroethylene |
title_full |
Production of 11alpha-hydroxyprogesterone using Aspergillus terreus immobilized on polytetrafluoroethylene |
title_fullStr |
Production of 11alpha-hydroxyprogesterone using Aspergillus terreus immobilized on polytetrafluoroethylene |
title_full_unstemmed |
Production of 11alpha-hydroxyprogesterone using Aspergillus terreus immobilized on polytetrafluoroethylene |
title_sort |
Production of 11alpha-hydroxyprogesterone using Aspergillus terreus immobilized on polytetrafluoroethylene |
author |
Ahmed,E.M. |
author_facet |
Ahmed,E.M. |
author_role |
author |
dc.contributor.author.fl_str_mv |
Ahmed,E.M. |
dc.subject.por.fl_str_mv |
11alpha-hydroxprogesterone polytetrafluoroethylene Aspergillus terreus |
topic |
11alpha-hydroxprogesterone polytetrafluoroethylene Aspergillus terreus |
description |
In attempt to immobilize the mycelial fungi on a new hydrophobic matrix, Aspergillus terreus mycelia were immobilized into polytetrafluoroethylen (PTFE) and used for selected conversion of progesterone to 11alpha-hydroxyprogesterone. The immobilized cells exhibited higher yields than the free cells. Different parameters that can affect the cell activity were measured. The optimum pH was 6 and the optimum temperature was 30ºC. Higher transformation activities were achieved with matrix contained 40% PTFE. No considerable loss in yield was observed with storage of the cells at 4ºC for 30 days. Utilization of the immobilized cells in repeated batch processes indicated that the cells retained about 84% of their activity after reuse for eight successive cycles. |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000200007 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000200007 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1517-83822007000200007 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
dc.source.none.fl_str_mv |
Brazilian Journal of Microbiology v.38 n.2 2007 reponame:Brazilian Journal of Microbiology instname:Sociedade Brasileira de Microbiologia (SBM) instacron:SBM |
instname_str |
Sociedade Brasileira de Microbiologia (SBM) |
instacron_str |
SBM |
institution |
SBM |
reponame_str |
Brazilian Journal of Microbiology |
collection |
Brazilian Journal of Microbiology |
repository.name.fl_str_mv |
Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM) |
repository.mail.fl_str_mv |
bjm@sbmicrobiologia.org.br||mbmartin@usp.br |
_version_ |
1752122201015844864 |