Nested-PCR using MPB64 fragment improves the diagnosis of pleural and meningeal tuberculosis
Autor(a) principal: | |
---|---|
Data de Publicação: | 2000 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista da Sociedade Brasileira de Medicina Tropical |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822000000300003 |
Resumo: | Fluids in which Mycobacterium tuberculosis are seldom found, such as pleural and cerebrospinal liquids, are good candidates to be studied using PCR techniques. We detail our experience with a PCR assay applied to pleural and cerebrospinal fluids using the primer MPB64. Seventy three specimens were analyzed: 30 pleural fluids (PF), 26 pleural biopsies (PB) and 17 cerebrospinal fluids (CSF). The gold standard for the diagnosis of tuberculous meningitis was the positive culture for M. tuberculosis in CSF. Tuberculous pleural effusion was diagnosed when cultures of PF and/or PB were positive for M. tuberculosis, or the PB histology showed granulomas. Our results, compared to the gold standards employed, showed a sensitivity of 70%, specificity of 88%, positive predictive value of 82% and negative predictive value of 80%. The high specificity of the MPB64 fragment while still retaining a good sensitivity makes it very well suited for pleural and cerebrospinal tuberculosis diagnosis. |
id |
SBMT-1_267cc295aaa822c0a9acc43206556fa0 |
---|---|
oai_identifier_str |
oai:scielo:S0037-86822000000300003 |
network_acronym_str |
SBMT-1 |
network_name_str |
Revista da Sociedade Brasileira de Medicina Tropical |
repository_id_str |
|
spelling |
Nested-PCR using MPB64 fragment improves the diagnosis of pleural and meningeal tuberculosisTuberculosisDiagnosisPCR techniqueCentral nervous systemPleuraFluids in which Mycobacterium tuberculosis are seldom found, such as pleural and cerebrospinal liquids, are good candidates to be studied using PCR techniques. We detail our experience with a PCR assay applied to pleural and cerebrospinal fluids using the primer MPB64. Seventy three specimens were analyzed: 30 pleural fluids (PF), 26 pleural biopsies (PB) and 17 cerebrospinal fluids (CSF). The gold standard for the diagnosis of tuberculous meningitis was the positive culture for M. tuberculosis in CSF. Tuberculous pleural effusion was diagnosed when cultures of PF and/or PB were positive for M. tuberculosis, or the PB histology showed granulomas. Our results, compared to the gold standards employed, showed a sensitivity of 70%, specificity of 88%, positive predictive value of 82% and negative predictive value of 80%. The high specificity of the MPB64 fragment while still retaining a good sensitivity makes it very well suited for pleural and cerebrospinal tuberculosis diagnosis.Sociedade Brasileira de Medicina Tropical - SBMT2000-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822000000300003Revista da Sociedade Brasileira de Medicina Tropical v.33 n.3 2000reponame:Revista da Sociedade Brasileira de Medicina Tropicalinstname:Sociedade Brasileira de Medicina Tropical (SBMT)instacron:SBMT10.1590/S0037-86822000000300003info:eu-repo/semantics/openAccessMartins,Luiz C.Paschoal,Ilma A.Von Nowakonski,AngelaSilva,Silvana A.B.Costa,Fernando F.Ward,Laura Sterianeng2000-08-22T00:00:00Zoai:scielo:S0037-86822000000300003Revistahttps://www.sbmt.org.br/portal/revista/ONGhttps://old.scielo.br/oai/scielo-oai.php||dalmo@rsbmt.uftm.edu.br|| rsbmt@rsbmt.uftm.edu.br1678-98490037-8682opendoar:2000-08-22T00:00Revista da Sociedade Brasileira de Medicina Tropical - Sociedade Brasileira de Medicina Tropical (SBMT)false |
dc.title.none.fl_str_mv |
Nested-PCR using MPB64 fragment improves the diagnosis of pleural and meningeal tuberculosis |
title |
Nested-PCR using MPB64 fragment improves the diagnosis of pleural and meningeal tuberculosis |
spellingShingle |
Nested-PCR using MPB64 fragment improves the diagnosis of pleural and meningeal tuberculosis Martins,Luiz C. Tuberculosis Diagnosis PCR technique Central nervous system Pleura |
title_short |
Nested-PCR using MPB64 fragment improves the diagnosis of pleural and meningeal tuberculosis |
title_full |
Nested-PCR using MPB64 fragment improves the diagnosis of pleural and meningeal tuberculosis |
title_fullStr |
Nested-PCR using MPB64 fragment improves the diagnosis of pleural and meningeal tuberculosis |
title_full_unstemmed |
Nested-PCR using MPB64 fragment improves the diagnosis of pleural and meningeal tuberculosis |
title_sort |
Nested-PCR using MPB64 fragment improves the diagnosis of pleural and meningeal tuberculosis |
author |
Martins,Luiz C. |
author_facet |
Martins,Luiz C. Paschoal,Ilma A. Von Nowakonski,Angela Silva,Silvana A.B. Costa,Fernando F. Ward,Laura Sterian |
author_role |
author |
author2 |
Paschoal,Ilma A. Von Nowakonski,Angela Silva,Silvana A.B. Costa,Fernando F. Ward,Laura Sterian |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Martins,Luiz C. Paschoal,Ilma A. Von Nowakonski,Angela Silva,Silvana A.B. Costa,Fernando F. Ward,Laura Sterian |
dc.subject.por.fl_str_mv |
Tuberculosis Diagnosis PCR technique Central nervous system Pleura |
topic |
Tuberculosis Diagnosis PCR technique Central nervous system Pleura |
description |
Fluids in which Mycobacterium tuberculosis are seldom found, such as pleural and cerebrospinal liquids, are good candidates to be studied using PCR techniques. We detail our experience with a PCR assay applied to pleural and cerebrospinal fluids using the primer MPB64. Seventy three specimens were analyzed: 30 pleural fluids (PF), 26 pleural biopsies (PB) and 17 cerebrospinal fluids (CSF). The gold standard for the diagnosis of tuberculous meningitis was the positive culture for M. tuberculosis in CSF. Tuberculous pleural effusion was diagnosed when cultures of PF and/or PB were positive for M. tuberculosis, or the PB histology showed granulomas. Our results, compared to the gold standards employed, showed a sensitivity of 70%, specificity of 88%, positive predictive value of 82% and negative predictive value of 80%. The high specificity of the MPB64 fragment while still retaining a good sensitivity makes it very well suited for pleural and cerebrospinal tuberculosis diagnosis. |
publishDate |
2000 |
dc.date.none.fl_str_mv |
2000-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822000000300003 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822000000300003 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0037-86822000000300003 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Medicina Tropical - SBMT |
publisher.none.fl_str_mv |
Sociedade Brasileira de Medicina Tropical - SBMT |
dc.source.none.fl_str_mv |
Revista da Sociedade Brasileira de Medicina Tropical v.33 n.3 2000 reponame:Revista da Sociedade Brasileira de Medicina Tropical instname:Sociedade Brasileira de Medicina Tropical (SBMT) instacron:SBMT |
instname_str |
Sociedade Brasileira de Medicina Tropical (SBMT) |
instacron_str |
SBMT |
institution |
SBMT |
reponame_str |
Revista da Sociedade Brasileira de Medicina Tropical |
collection |
Revista da Sociedade Brasileira de Medicina Tropical |
repository.name.fl_str_mv |
Revista da Sociedade Brasileira de Medicina Tropical - Sociedade Brasileira de Medicina Tropical (SBMT) |
repository.mail.fl_str_mv |
||dalmo@rsbmt.uftm.edu.br|| rsbmt@rsbmt.uftm.edu.br |
_version_ |
1752122151104675840 |