Epidemiological aspects of Toxoplasma gondii infection in riverside communities in the Southern Brazilian Amazon
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista da Sociedade Brasileira de Medicina Tropical |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822015000300301 |
Resumo: | INTRODUCTION: Toxoplasma gondii infection is widely prevalent in humans and other animals worldwide. Information on the prevalence of T. gondii infection is scarce in some regions of Brazil, including riverside communities along the Amazon River basin. M METHODS: The prevalence of T. gondii in 231 people, aged 1-85 years, who were living in four riverside communities along the Purus River, Lábrea, State of Amazonas, Brazil, was determined. Antibodies against T. gondii were assayed using a commercial enzyme-linked immunosorbent assay (ELISA) kit. The hearts and brains of 50 chickens, which were raised free-range in the communities, were pooled according to the community of origin and bioassayed in mice. The isolates were genotyped using polymorphisms at 12 nuclear markers (SAG1, 5' and 3'-SAG2, alt.SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, Apico and CS3). RESULTS: The overall seroprevalence of T. gondii was 56.7% (131/231). IgG antibodies were presented by 117 (89.3%) and IgM by 14 (10.7%) of the 131 positive individuals. No association between age group and gender with prevalence was observed (chi-square test, p > 0.05); however, the comparison between localities showed that the seroprevalence of T. gondii was significantly lower among the individuals living in the Boca do Ituxi (p < 0.05) community. Five isolates of T. gondii were obtained in the mouse bioassay, and genotyping revealed two complete genotypes that had not been described previously and three mixed isolates. CONCLUSIONS: These results support previous findings that T. gondii population genetics are highly diverse in Brazil and that T. gondii infection is active in these riverside communities. |
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Epidemiological aspects of Toxoplasma gondii infection in riverside communities in the Southern Brazilian AmazonToxoplasma gondiiRiverside communitiesAmazonPurus RiverEpidemiology INTRODUCTION: Toxoplasma gondii infection is widely prevalent in humans and other animals worldwide. Information on the prevalence of T. gondii infection is scarce in some regions of Brazil, including riverside communities along the Amazon River basin. M METHODS: The prevalence of T. gondii in 231 people, aged 1-85 years, who were living in four riverside communities along the Purus River, Lábrea, State of Amazonas, Brazil, was determined. Antibodies against T. gondii were assayed using a commercial enzyme-linked immunosorbent assay (ELISA) kit. The hearts and brains of 50 chickens, which were raised free-range in the communities, were pooled according to the community of origin and bioassayed in mice. The isolates were genotyped using polymorphisms at 12 nuclear markers (SAG1, 5' and 3'-SAG2, alt.SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, Apico and CS3). RESULTS: The overall seroprevalence of T. gondii was 56.7% (131/231). IgG antibodies were presented by 117 (89.3%) and IgM by 14 (10.7%) of the 131 positive individuals. No association between age group and gender with prevalence was observed (chi-square test, p > 0.05); however, the comparison between localities showed that the seroprevalence of T. gondii was significantly lower among the individuals living in the Boca do Ituxi (p < 0.05) community. Five isolates of T. gondii were obtained in the mouse bioassay, and genotyping revealed two complete genotypes that had not been described previously and three mixed isolates. CONCLUSIONS: These results support previous findings that T. gondii population genetics are highly diverse in Brazil and that T. gondii infection is active in these riverside communities. Sociedade Brasileira de Medicina Tropical - SBMT2015-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822015000300301Revista da Sociedade Brasileira de Medicina Tropical v.48 n.3 2015reponame:Revista da Sociedade Brasileira de Medicina Tropicalinstname:Sociedade Brasileira de Medicina Tropical (SBMT)instacron:SBMT10.1590/0037-8682-0040-2015info:eu-repo/semantics/openAccessVitaliano,Sérgio NetoMendonça,Gabriel Maciel deSandres,Felipe Amsterdam Maia deCamargo,Juliana de Souza Almeida AranhaTarso,Paulo deBasano,Sérgio de AlmeidaSilva,Jéssica Carolinne Damasceno eSouza,Viviane Krominski Graça deCartonilho,GlenciAlmeida,Alexandre Thomé da Silva deGennari,Solange MariaCamargo,Luís Marcelo Aranhaeng2015-06-18T00:00:00Zoai:scielo:S0037-86822015000300301Revistahttps://www.sbmt.org.br/portal/revista/ONGhttps://old.scielo.br/oai/scielo-oai.php||dalmo@rsbmt.uftm.edu.br|| rsbmt@rsbmt.uftm.edu.br1678-98490037-8682opendoar:2015-06-18T00:00Revista da Sociedade Brasileira de Medicina Tropical - Sociedade Brasileira de Medicina Tropical (SBMT)false |
dc.title.none.fl_str_mv |
Epidemiological aspects of Toxoplasma gondii infection in riverside communities in the Southern Brazilian Amazon |
title |
Epidemiological aspects of Toxoplasma gondii infection in riverside communities in the Southern Brazilian Amazon |
spellingShingle |
Epidemiological aspects of Toxoplasma gondii infection in riverside communities in the Southern Brazilian Amazon Vitaliano,Sérgio Neto Toxoplasma gondii Riverside communities Amazon Purus River Epidemiology |
title_short |
Epidemiological aspects of Toxoplasma gondii infection in riverside communities in the Southern Brazilian Amazon |
title_full |
Epidemiological aspects of Toxoplasma gondii infection in riverside communities in the Southern Brazilian Amazon |
title_fullStr |
Epidemiological aspects of Toxoplasma gondii infection in riverside communities in the Southern Brazilian Amazon |
title_full_unstemmed |
Epidemiological aspects of Toxoplasma gondii infection in riverside communities in the Southern Brazilian Amazon |
title_sort |
Epidemiological aspects of Toxoplasma gondii infection in riverside communities in the Southern Brazilian Amazon |
author |
Vitaliano,Sérgio Neto |
author_facet |
Vitaliano,Sérgio Neto Mendonça,Gabriel Maciel de Sandres,Felipe Amsterdam Maia de Camargo,Juliana de Souza Almeida Aranha Tarso,Paulo de Basano,Sérgio de Almeida Silva,Jéssica Carolinne Damasceno e Souza,Viviane Krominski Graça de Cartonilho,Glenci Almeida,Alexandre Thomé da Silva de Gennari,Solange Maria Camargo,Luís Marcelo Aranha |
author_role |
author |
author2 |
Mendonça,Gabriel Maciel de Sandres,Felipe Amsterdam Maia de Camargo,Juliana de Souza Almeida Aranha Tarso,Paulo de Basano,Sérgio de Almeida Silva,Jéssica Carolinne Damasceno e Souza,Viviane Krominski Graça de Cartonilho,Glenci Almeida,Alexandre Thomé da Silva de Gennari,Solange Maria Camargo,Luís Marcelo Aranha |
author2_role |
author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Vitaliano,Sérgio Neto Mendonça,Gabriel Maciel de Sandres,Felipe Amsterdam Maia de Camargo,Juliana de Souza Almeida Aranha Tarso,Paulo de Basano,Sérgio de Almeida Silva,Jéssica Carolinne Damasceno e Souza,Viviane Krominski Graça de Cartonilho,Glenci Almeida,Alexandre Thomé da Silva de Gennari,Solange Maria Camargo,Luís Marcelo Aranha |
dc.subject.por.fl_str_mv |
Toxoplasma gondii Riverside communities Amazon Purus River Epidemiology |
topic |
Toxoplasma gondii Riverside communities Amazon Purus River Epidemiology |
description |
INTRODUCTION: Toxoplasma gondii infection is widely prevalent in humans and other animals worldwide. Information on the prevalence of T. gondii infection is scarce in some regions of Brazil, including riverside communities along the Amazon River basin. M METHODS: The prevalence of T. gondii in 231 people, aged 1-85 years, who were living in four riverside communities along the Purus River, Lábrea, State of Amazonas, Brazil, was determined. Antibodies against T. gondii were assayed using a commercial enzyme-linked immunosorbent assay (ELISA) kit. The hearts and brains of 50 chickens, which were raised free-range in the communities, were pooled according to the community of origin and bioassayed in mice. The isolates were genotyped using polymorphisms at 12 nuclear markers (SAG1, 5' and 3'-SAG2, alt.SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, Apico and CS3). RESULTS: The overall seroprevalence of T. gondii was 56.7% (131/231). IgG antibodies were presented by 117 (89.3%) and IgM by 14 (10.7%) of the 131 positive individuals. No association between age group and gender with prevalence was observed (chi-square test, p > 0.05); however, the comparison between localities showed that the seroprevalence of T. gondii was significantly lower among the individuals living in the Boca do Ituxi (p < 0.05) community. Five isolates of T. gondii were obtained in the mouse bioassay, and genotyping revealed two complete genotypes that had not been described previously and three mixed isolates. CONCLUSIONS: These results support previous findings that T. gondii population genetics are highly diverse in Brazil and that T. gondii infection is active in these riverside communities. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822015000300301 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822015000300301 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0037-8682-0040-2015 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Medicina Tropical - SBMT |
publisher.none.fl_str_mv |
Sociedade Brasileira de Medicina Tropical - SBMT |
dc.source.none.fl_str_mv |
Revista da Sociedade Brasileira de Medicina Tropical v.48 n.3 2015 reponame:Revista da Sociedade Brasileira de Medicina Tropical instname:Sociedade Brasileira de Medicina Tropical (SBMT) instacron:SBMT |
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Sociedade Brasileira de Medicina Tropical (SBMT) |
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SBMT |
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SBMT |
reponame_str |
Revista da Sociedade Brasileira de Medicina Tropical |
collection |
Revista da Sociedade Brasileira de Medicina Tropical |
repository.name.fl_str_mv |
Revista da Sociedade Brasileira de Medicina Tropical - Sociedade Brasileira de Medicina Tropical (SBMT) |
repository.mail.fl_str_mv |
||dalmo@rsbmt.uftm.edu.br|| rsbmt@rsbmt.uftm.edu.br |
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