MOLECULAR DIAGNOSIS OF LEPTOSPIROSIS IN BLOOD OF DOGS NATURALLY INFECTED

Detalhes bibliográficos
Autor(a) principal: Meira, Caroline Dantas
Data de Publicação: 2011
Outros Autores: Wenceslau, Amauri Arias, Carvalho, Fábio Santos, Albuquerque, George Rêgo, Dias, Roberta Costa
Tipo de documento: Artigo
Idioma: por
Título da fonte: Brazilian Journal of Veterinary Medicine
Texto Completo: https://rbmv.org/BJVM/article/view/777
Resumo: ABSTRACT. Meira C.D., Wenceslau A.A., Carvalho F.S., Albuquerque, G.R. & Dias R.C. Molecular diagnosis of Leptospirosis in blood of dogs naturally infected. [Diagnóstico molecular de leptospirose no sangue de cães naturalmente infectados]. Revista Brasileira de Medicina Veterinária, 33(1):7-11, 2011. Programa de Pós-Graduação em Ciência Animal, Universidade Estadual de Santa Cruz, Rodovia Ilhéus-Itabuna, km 16, Ilheús, BA 45662-900, Brasil. E-mail: wenceslau@uesc.br Leptospirosis is an acute and systemic infectious disease, which affects men and animals and is caused by pathogenic spirochetes of the genus Leptospira spp. The early diagnosis is important for the adoption of the appropriate therapy and prevention against the installation of critical symptomatologic forms. The Polymerase Chain Reaction (PCR) is being used for the diagnosis of infectious diseases showing efficiency for the diagnosis of the disease before the development of antibodies. The aim of this work was to evaluate the PCR technique with the primers G1 and G2 that amplify a fragment of 285 base pairs, specific of the region of gene secY of Leptospira spp, on 200 blood samples from domestic and stray dogs and determination of the risk factors associated from the Municipality of Ilhéus in the State of Bahia. Nine animals (4.5%) presented positive results for leptospirosis. There was no statistically significant difference between the prevalence of leptospirosis in domiciled or stray dogs (p= 1.0), sex (p= 0.455), age (p=0.644), access to the street (p= 0.113) and presence and/or contact with rodents (p= 0.362).
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spelling MOLECULAR DIAGNOSIS OF LEPTOSPIROSIS IN BLOOD OF DOGS NATURALLY INFECTEDDIAGNÓSTICO MOLECULAR DE LEPTOSPIROSE NO SANGUE DE CÃES NATURALMENTE INFECTADOSDNALeptospirazoonoseDNALeptospiraZoonosisdogsABSTRACT. Meira C.D., Wenceslau A.A., Carvalho F.S., Albuquerque, G.R. & Dias R.C. Molecular diagnosis of Leptospirosis in blood of dogs naturally infected. [Diagnóstico molecular de leptospirose no sangue de cães naturalmente infectados]. Revista Brasileira de Medicina Veterinária, 33(1):7-11, 2011. Programa de Pós-Graduação em Ciência Animal, Universidade Estadual de Santa Cruz, Rodovia Ilhéus-Itabuna, km 16, Ilheús, BA 45662-900, Brasil. E-mail: wenceslau@uesc.br Leptospirosis is an acute and systemic infectious disease, which affects men and animals and is caused by pathogenic spirochetes of the genus Leptospira spp. The early diagnosis is important for the adoption of the appropriate therapy and prevention against the installation of critical symptomatologic forms. The Polymerase Chain Reaction (PCR) is being used for the diagnosis of infectious diseases showing efficiency for the diagnosis of the disease before the development of antibodies. The aim of this work was to evaluate the PCR technique with the primers G1 and G2 that amplify a fragment of 285 base pairs, specific of the region of gene secY of Leptospira spp, on 200 blood samples from domestic and stray dogs and determination of the risk factors associated from the Municipality of Ilhéus in the State of Bahia. Nine animals (4.5%) presented positive results for leptospirosis. There was no statistically significant difference between the prevalence of leptospirosis in domiciled or stray dogs (p= 1.0), sex (p= 0.455), age (p=0.644), access to the street (p= 0.113) and presence and/or contact with rodents (p= 0.362).A Leptospirose é uma doença aguda e infecciosa sistêmica, que acomete homens e animais e é causada por espiroquetas patogênicas do gênero Leptospira spp. O diagnóstico precoce é importante para a adoção da terapêutica adequada e prevenção contra a instalação de formas sintomatológicas críticas. A Reação em Cadeia da Polimerase (PCR) está sendo usada para diagnóstico de doenças infecciosas, mostrando eficiência para o diagnóstico da doença antes do desenvolvimento de anticorpos. O objetivo deste trabalho foi avaliar a técnica de PCR com os iniciadores G1 e G2, que amplificam um fragmento de 285 pares de base, específicas da região do gene secY de Leptospira spp., em 200 amostras de sangue de cães domiciliados e vadios e à determinação do fatores de risco associados do município de Ilhéus, Estado da Bahia. Desses animais, nove (4,5%) apresentaram resultados positivos para leptospirose. Não houve diferença estatisticamente significativa entre a prevalência de leptospirose em cães domiciliados ou errantes (p = 1,0), sexo (p = 0,455), idade (p = 0,644), o acesso à rua (p = 0,113) e a presença e/ou contato com roedores (p = 0,362).Sociedade de Medicina Veterinária do Estado do Rio de Janeiro.2011-03-31info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionpeer reviewedAvaliado pelos paresapplication/pdfhttps://rbmv.org/BJVM/article/view/777Brazilian Journal of Veterinary Medicine; Vol. 33 No. 1 (2011); 7-11Revista Brasileira de Medicina Veterinária; v. 33 n. 1 (2011); 7-112527-21790100-2430reponame:Brazilian Journal of Veterinary Medicineinstname:Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ)instacron:SBMVporhttps://rbmv.org/BJVM/article/view/777/636Meira, Caroline DantasWenceslau, Amauri AriasCarvalho, Fábio SantosAlbuquerque, George RêgoDias, Roberta Costainfo:eu-repo/semantics/openAccess2020-12-23T17:27:55Zoai:ojs.rbmv.org:article/777Revistahttps://rbmv.org/BJVMONGhttps://rbmv.org/BJVM/oaicontato.rbmv@gmail.com2527-21790100-2430opendoar:2020-12-23T17:27:55Brazilian Journal of Veterinary Medicine - Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ)false
dc.title.none.fl_str_mv MOLECULAR DIAGNOSIS OF LEPTOSPIROSIS IN BLOOD OF DOGS NATURALLY INFECTED
DIAGNÓSTICO MOLECULAR DE LEPTOSPIROSE NO SANGUE DE CÃES NATURALMENTE INFECTADOS
title MOLECULAR DIAGNOSIS OF LEPTOSPIROSIS IN BLOOD OF DOGS NATURALLY INFECTED
spellingShingle MOLECULAR DIAGNOSIS OF LEPTOSPIROSIS IN BLOOD OF DOGS NATURALLY INFECTED
Meira, Caroline Dantas
DNA
Leptospira
zoonose
DNA
Leptospira
Zoonosis
dogs
title_short MOLECULAR DIAGNOSIS OF LEPTOSPIROSIS IN BLOOD OF DOGS NATURALLY INFECTED
title_full MOLECULAR DIAGNOSIS OF LEPTOSPIROSIS IN BLOOD OF DOGS NATURALLY INFECTED
title_fullStr MOLECULAR DIAGNOSIS OF LEPTOSPIROSIS IN BLOOD OF DOGS NATURALLY INFECTED
title_full_unstemmed MOLECULAR DIAGNOSIS OF LEPTOSPIROSIS IN BLOOD OF DOGS NATURALLY INFECTED
title_sort MOLECULAR DIAGNOSIS OF LEPTOSPIROSIS IN BLOOD OF DOGS NATURALLY INFECTED
author Meira, Caroline Dantas
author_facet Meira, Caroline Dantas
Wenceslau, Amauri Arias
Carvalho, Fábio Santos
Albuquerque, George Rêgo
Dias, Roberta Costa
author_role author
author2 Wenceslau, Amauri Arias
Carvalho, Fábio Santos
Albuquerque, George Rêgo
Dias, Roberta Costa
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Meira, Caroline Dantas
Wenceslau, Amauri Arias
Carvalho, Fábio Santos
Albuquerque, George Rêgo
Dias, Roberta Costa
dc.subject.por.fl_str_mv DNA
Leptospira
zoonose
DNA
Leptospira
Zoonosis
dogs
topic DNA
Leptospira
zoonose
DNA
Leptospira
Zoonosis
dogs
description ABSTRACT. Meira C.D., Wenceslau A.A., Carvalho F.S., Albuquerque, G.R. & Dias R.C. Molecular diagnosis of Leptospirosis in blood of dogs naturally infected. [Diagnóstico molecular de leptospirose no sangue de cães naturalmente infectados]. Revista Brasileira de Medicina Veterinária, 33(1):7-11, 2011. Programa de Pós-Graduação em Ciência Animal, Universidade Estadual de Santa Cruz, Rodovia Ilhéus-Itabuna, km 16, Ilheús, BA 45662-900, Brasil. E-mail: wenceslau@uesc.br Leptospirosis is an acute and systemic infectious disease, which affects men and animals and is caused by pathogenic spirochetes of the genus Leptospira spp. The early diagnosis is important for the adoption of the appropriate therapy and prevention against the installation of critical symptomatologic forms. The Polymerase Chain Reaction (PCR) is being used for the diagnosis of infectious diseases showing efficiency for the diagnosis of the disease before the development of antibodies. The aim of this work was to evaluate the PCR technique with the primers G1 and G2 that amplify a fragment of 285 base pairs, specific of the region of gene secY of Leptospira spp, on 200 blood samples from domestic and stray dogs and determination of the risk factors associated from the Municipality of Ilhéus in the State of Bahia. Nine animals (4.5%) presented positive results for leptospirosis. There was no statistically significant difference between the prevalence of leptospirosis in domiciled or stray dogs (p= 1.0), sex (p= 0.455), age (p=0.644), access to the street (p= 0.113) and presence and/or contact with rodents (p= 0.362).
publishDate 2011
dc.date.none.fl_str_mv 2011-03-31
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
peer reviewed
Avaliado pelos pares
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rbmv.org/BJVM/article/view/777
url https://rbmv.org/BJVM/article/view/777
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://rbmv.org/BJVM/article/view/777/636
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Sociedade de Medicina Veterinária do Estado do Rio de Janeiro.
publisher.none.fl_str_mv Sociedade de Medicina Veterinária do Estado do Rio de Janeiro.
dc.source.none.fl_str_mv Brazilian Journal of Veterinary Medicine; Vol. 33 No. 1 (2011); 7-11
Revista Brasileira de Medicina Veterinária; v. 33 n. 1 (2011); 7-11
2527-2179
0100-2430
reponame:Brazilian Journal of Veterinary Medicine
instname:Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ)
instacron:SBMV
instname_str Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ)
instacron_str SBMV
institution SBMV
reponame_str Brazilian Journal of Veterinary Medicine
collection Brazilian Journal of Veterinary Medicine
repository.name.fl_str_mv Brazilian Journal of Veterinary Medicine - Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ)
repository.mail.fl_str_mv contato.rbmv@gmail.com
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