Comparison between the techniques of inclusion in glycol methacrylate (GMA)- based plastic resin and paraffin for evaluation intestinal morphometry in horses
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Veterinary Medicine |
Texto Completo: | https://rbmv.org/BJVM/article/view/1226 |
Resumo: | To determine the effect of the inclusion method on the histomorphometric evaluation of the gastrointestinal mucosa of horses, jejunum samples were collected using flank laparotomy. Sixteen mixed breed healthy adult horses, including four males and 12 females, aged 4–14 years with an average body weight of 248.40 ± 2.28 kg, were used. Jejunal biopsies were collected and analyzed by light microscopy using two methods: group 1 comprised biopsies fixed using 10% neutral formalin and embedded in paraffin; biopsies in group 2 were fixed in 2.5% glutaraldehyde in 0.1 M phosphate buffer pH 7.2, followed by inclusion in glycol methacrylate (GMA)-based plastic resin. Intestinal villi height, crypt depth, glandular mucosa thickness, total mucosal thickness, and villus/crypt ratio were then evaluated. For all the variables studied, with exception of the villus/crypt ratio, a significant difference (p < 0.05) was found between samples in groups 1 and 2. Processing samples for embedding in plastic resin was quicker and easier to perform compared to that for paraffin embedding. In addition, the epithelial lining of tissues in group 2 showed better resolution for conducting cytological studies under a light microscope. The difference between the studied variables can be attributed to tissue retraction caused by conventional processing for inclusion in paraffin. Therefore, the method of inclusion in GMA described in the present study appears to be a more reliable choice for morphometric evaluation of the intestinal mucosa of horses. |
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Comparison between the techniques of inclusion in glycol methacrylate (GMA)- based plastic resin and paraffin for evaluation intestinal morphometry in horsesComparação entre as técnicas de inclusão em resina plástica à base de glicol metacrilato (GMA) e inclusão em parafina para avaliação da morfometria intestinal em equinosbiopsy, equine, intestinal mucosa, small intestine, villi heightbiópsias, equino, mucosa intestinal, intestino delgado, altura de vilosidade.To determine the effect of the inclusion method on the histomorphometric evaluation of the gastrointestinal mucosa of horses, jejunum samples were collected using flank laparotomy. Sixteen mixed breed healthy adult horses, including four males and 12 females, aged 4–14 years with an average body weight of 248.40 ± 2.28 kg, were used. Jejunal biopsies were collected and analyzed by light microscopy using two methods: group 1 comprised biopsies fixed using 10% neutral formalin and embedded in paraffin; biopsies in group 2 were fixed in 2.5% glutaraldehyde in 0.1 M phosphate buffer pH 7.2, followed by inclusion in glycol methacrylate (GMA)-based plastic resin. Intestinal villi height, crypt depth, glandular mucosa thickness, total mucosal thickness, and villus/crypt ratio were then evaluated. For all the variables studied, with exception of the villus/crypt ratio, a significant difference (p < 0.05) was found between samples in groups 1 and 2. Processing samples for embedding in plastic resin was quicker and easier to perform compared to that for paraffin embedding. In addition, the epithelial lining of tissues in group 2 showed better resolution for conducting cytological studies under a light microscope. The difference between the studied variables can be attributed to tissue retraction caused by conventional processing for inclusion in paraffin. Therefore, the method of inclusion in GMA described in the present study appears to be a more reliable choice for morphometric evaluation of the intestinal mucosa of horses.Para determinar o efeito do método de inclusão sobre a avaliação histomorfométrica da mucosa gastrointestinal de equinos foram coletadas amostras do jejuno por laparotomia pelo flanco. Foram utilizados 16 equinos adultos hígidos, sem raça definida, de ambos os sexos, quatro machos e 12 fêmeas, com idade variando entre quatro e 14 anos, e peso corporal médio de 248,40 + 2,28kg. Amostras do jejuno foram coletadas e processadas para biopsia em microscopia de luz sob dois métodos: grupo 1 - fixação em formalina neutra tamponada a 10% e inclusão em parafina, grupo 2 - fixação em glutaraldeído 2,5% em tampão fosfato 0,1M pH 7,2, seguido de inclusão em resina plástica à base de glicol metacrilato. Os seguintes parâmetros foram avaliados: altura das vilosidades intestinais, profundidade de cripta, espessura da mucosa glandular, espessura total da mucosa e relação vilo/cripta. Para todas as variáveis estudadas, exceto relação vilo/cripta, foi encontrada diferença significativa (p<0,05) entre os dois métodos. O processamento para inclusão em resina plástica foi rápido e de fácil execução quando comparado à inclusão em parafina. Além disso, o epitélio de revestimento apresentou melhor resolução das células para estudo histológico ao microscópio de luz. A diferença entre as variáveis pode ser atribuída a retração do tecido provocada pelo processamento convencional por inclusão em parafina. Portanto, o método de inclusão em GMA mostrou-se, no presente estudo, uma escolha mais fidedigna para as avaliações morfométricas da mucosa intestinal de equinos.Sociedade de Medicina Veterinária do Estado do Rio de Janeiro.2022-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionpeer reviewedAvaliado pelos paresapplication/pdftext/xmlhttps://rbmv.org/BJVM/article/view/122610.29374/2527-2179.bjvm004521Brazilian Journal of Veterinary Medicine; Vol. 44 (2022); e004521Revista Brasileira de Medicina Veterinária; v. 44 (2022); e0045212527-21790100-2430reponame:Brazilian Journal of Veterinary Medicineinstname:Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ)instacron:SBMVenghttps://rbmv.org/BJVM/article/view/1226/1109https://rbmv.org/BJVM/article/view/1226/1165Copyright (c) 2022 Cíntia Ferreira, Maristela Silveira Palhares, Ubiratan Pereira de Melo, Helio Chiarini-Garcia, Renata de Pino Albuquerque Maranhão, Valentim Arabicano Gheller, Fabíola Oliveira Paes Lemehttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessFerreira, CíntiaPalhares, Maristela Silveirade Melo, Ubiratan PereiraChiarini-Garcia, HelioMaranhão, Renata de Pino AlbuquerqueGheller, Valentim ArabicanoLeme, Fabíola Oliveira Paes2022-06-25T21:48:19Zoai:ojs.rbmv.org:article/1226Revistahttps://rbmv.org/BJVMONGhttps://rbmv.org/BJVM/oaicontato.rbmv@gmail.com2527-21790100-2430opendoar:2022-06-25T21:48:19Brazilian Journal of Veterinary Medicine - Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ)false |
dc.title.none.fl_str_mv |
Comparison between the techniques of inclusion in glycol methacrylate (GMA)- based plastic resin and paraffin for evaluation intestinal morphometry in horses Comparação entre as técnicas de inclusão em resina plástica à base de glicol metacrilato (GMA) e inclusão em parafina para avaliação da morfometria intestinal em equinos |
title |
Comparison between the techniques of inclusion in glycol methacrylate (GMA)- based plastic resin and paraffin for evaluation intestinal morphometry in horses |
spellingShingle |
Comparison between the techniques of inclusion in glycol methacrylate (GMA)- based plastic resin and paraffin for evaluation intestinal morphometry in horses Ferreira, Cíntia biopsy, equine, intestinal mucosa, small intestine, villi height biópsias, equino, mucosa intestinal, intestino delgado, altura de vilosidade. |
title_short |
Comparison between the techniques of inclusion in glycol methacrylate (GMA)- based plastic resin and paraffin for evaluation intestinal morphometry in horses |
title_full |
Comparison between the techniques of inclusion in glycol methacrylate (GMA)- based plastic resin and paraffin for evaluation intestinal morphometry in horses |
title_fullStr |
Comparison between the techniques of inclusion in glycol methacrylate (GMA)- based plastic resin and paraffin for evaluation intestinal morphometry in horses |
title_full_unstemmed |
Comparison between the techniques of inclusion in glycol methacrylate (GMA)- based plastic resin and paraffin for evaluation intestinal morphometry in horses |
title_sort |
Comparison between the techniques of inclusion in glycol methacrylate (GMA)- based plastic resin and paraffin for evaluation intestinal morphometry in horses |
author |
Ferreira, Cíntia |
author_facet |
Ferreira, Cíntia Palhares, Maristela Silveira de Melo, Ubiratan Pereira Chiarini-Garcia, Helio Maranhão, Renata de Pino Albuquerque Gheller, Valentim Arabicano Leme, Fabíola Oliveira Paes |
author_role |
author |
author2 |
Palhares, Maristela Silveira de Melo, Ubiratan Pereira Chiarini-Garcia, Helio Maranhão, Renata de Pino Albuquerque Gheller, Valentim Arabicano Leme, Fabíola Oliveira Paes |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Ferreira, Cíntia Palhares, Maristela Silveira de Melo, Ubiratan Pereira Chiarini-Garcia, Helio Maranhão, Renata de Pino Albuquerque Gheller, Valentim Arabicano Leme, Fabíola Oliveira Paes |
dc.subject.por.fl_str_mv |
biopsy, equine, intestinal mucosa, small intestine, villi height biópsias, equino, mucosa intestinal, intestino delgado, altura de vilosidade. |
topic |
biopsy, equine, intestinal mucosa, small intestine, villi height biópsias, equino, mucosa intestinal, intestino delgado, altura de vilosidade. |
description |
To determine the effect of the inclusion method on the histomorphometric evaluation of the gastrointestinal mucosa of horses, jejunum samples were collected using flank laparotomy. Sixteen mixed breed healthy adult horses, including four males and 12 females, aged 4–14 years with an average body weight of 248.40 ± 2.28 kg, were used. Jejunal biopsies were collected and analyzed by light microscopy using two methods: group 1 comprised biopsies fixed using 10% neutral formalin and embedded in paraffin; biopsies in group 2 were fixed in 2.5% glutaraldehyde in 0.1 M phosphate buffer pH 7.2, followed by inclusion in glycol methacrylate (GMA)-based plastic resin. Intestinal villi height, crypt depth, glandular mucosa thickness, total mucosal thickness, and villus/crypt ratio were then evaluated. For all the variables studied, with exception of the villus/crypt ratio, a significant difference (p < 0.05) was found between samples in groups 1 and 2. Processing samples for embedding in plastic resin was quicker and easier to perform compared to that for paraffin embedding. In addition, the epithelial lining of tissues in group 2 showed better resolution for conducting cytological studies under a light microscope. The difference between the studied variables can be attributed to tissue retraction caused by conventional processing for inclusion in paraffin. Therefore, the method of inclusion in GMA described in the present study appears to be a more reliable choice for morphometric evaluation of the intestinal mucosa of horses. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-02-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion peer reviewed Avaliado pelos pares |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rbmv.org/BJVM/article/view/1226 10.29374/2527-2179.bjvm004521 |
url |
https://rbmv.org/BJVM/article/view/1226 |
identifier_str_mv |
10.29374/2527-2179.bjvm004521 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://rbmv.org/BJVM/article/view/1226/1109 https://rbmv.org/BJVM/article/view/1226/1165 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf text/xml |
dc.publisher.none.fl_str_mv |
Sociedade de Medicina Veterinária do Estado do Rio de Janeiro. |
publisher.none.fl_str_mv |
Sociedade de Medicina Veterinária do Estado do Rio de Janeiro. |
dc.source.none.fl_str_mv |
Brazilian Journal of Veterinary Medicine; Vol. 44 (2022); e004521 Revista Brasileira de Medicina Veterinária; v. 44 (2022); e004521 2527-2179 0100-2430 reponame:Brazilian Journal of Veterinary Medicine instname:Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ) instacron:SBMV |
instname_str |
Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ) |
instacron_str |
SBMV |
institution |
SBMV |
reponame_str |
Brazilian Journal of Veterinary Medicine |
collection |
Brazilian Journal of Veterinary Medicine |
repository.name.fl_str_mv |
Brazilian Journal of Veterinary Medicine - Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ) |
repository.mail.fl_str_mv |
contato.rbmv@gmail.com |
_version_ |
1798313111655022592 |