Kint3-4 protein from human plasminogen delays Ehrlich tumor growth in mice
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Jornal Brasileiro de Patologia e Medicina Laboratorial (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1676-24442011000400012 |
Resumo: | INTRODUCTION AND OBJECTIVE: Kint3-4 protein, originated from a genetic recombination of K1-3 and K1-4 human plasminogen segments, is recognized for its antiangiogenic and anti-inflammatory potential. This study aimed to evaluate the effect of Kint3-4 protein on tumor development in Swiss mice previously inoculated with Ehrlich tumor cells. METHODS: The protein fragment was obtained from Pichia pastoris cloning and transformation. After tumor cell inoculation three different protocols were used to assess tumor growth: beginning (0-6 days), peak (0-12 days) and after peak (0-18 days). We analyzed tumor growth, histomorphological characteristics and immunohistochemistry by use of CDC47 (cellular proliferation marker) and CD31 (blood vessel marker). RESULTS: Animals treated with Kint3-4 protein (150 µg/kg/48 h) showed lower tumor growth in all protocols. Based on histological assessment, inflammation and tumor areas were also reduced. Moreover, both the lowest rate of tumor cell proliferation and low microvessel density were observed in animals treated with Kint3-4 protein compared with the untreated control group. CONCLUSION: The effect of Kint3-4 recombinant protein on tumor angiogenesis and control of malignant cell proliferation enhances the prospects of its use in clinical and antiangiogenic treatment. |
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Kint3-4 protein from human plasminogen delays Ehrlich tumor growth in miceAngiogenesisCell proliferationAngiostatinNeoplasmINTRODUCTION AND OBJECTIVE: Kint3-4 protein, originated from a genetic recombination of K1-3 and K1-4 human plasminogen segments, is recognized for its antiangiogenic and anti-inflammatory potential. This study aimed to evaluate the effect of Kint3-4 protein on tumor development in Swiss mice previously inoculated with Ehrlich tumor cells. METHODS: The protein fragment was obtained from Pichia pastoris cloning and transformation. After tumor cell inoculation three different protocols were used to assess tumor growth: beginning (0-6 days), peak (0-12 days) and after peak (0-18 days). We analyzed tumor growth, histomorphological characteristics and immunohistochemistry by use of CDC47 (cellular proliferation marker) and CD31 (blood vessel marker). RESULTS: Animals treated with Kint3-4 protein (150 µg/kg/48 h) showed lower tumor growth in all protocols. Based on histological assessment, inflammation and tumor areas were also reduced. Moreover, both the lowest rate of tumor cell proliferation and low microvessel density were observed in animals treated with Kint3-4 protein compared with the untreated control group. CONCLUSION: The effect of Kint3-4 recombinant protein on tumor angiogenesis and control of malignant cell proliferation enhances the prospects of its use in clinical and antiangiogenic treatment.Sociedade Brasileira de Patologia Clínica2011-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1676-24442011000400012Jornal Brasileiro de Patologia e Medicina Laboratorial v.47 n.4 2011reponame:Jornal Brasileiro de Patologia e Medicina Laboratorial (Online)instname:Sociedade Brasileira de Patologia (SBP)instacron:SBP10.1590/S1676-24442011000400012info:eu-repo/semantics/openAccessSouza,Cristina Maria deFerreira,EnioFerreira,Mônica Alves Neves DinizAndrade,Silvia PassosSantos,Ivan CarlosPesquero,Jorge LuizCassali,Geovanni Dantaseng2011-09-14T00:00:00Zoai:scielo:S1676-24442011000400012Revistahttp://www.scielo.br/jbpmlhttps://old.scielo.br/oai/scielo-oai.php||jbpml@sbpc.org.br1678-47741676-2444opendoar:2011-09-14T00:00Jornal Brasileiro de Patologia e Medicina Laboratorial (Online) - Sociedade Brasileira de Patologia (SBP)false |
dc.title.none.fl_str_mv |
Kint3-4 protein from human plasminogen delays Ehrlich tumor growth in mice |
title |
Kint3-4 protein from human plasminogen delays Ehrlich tumor growth in mice |
spellingShingle |
Kint3-4 protein from human plasminogen delays Ehrlich tumor growth in mice Souza,Cristina Maria de Angiogenesis Cell proliferation Angiostatin Neoplasm |
title_short |
Kint3-4 protein from human plasminogen delays Ehrlich tumor growth in mice |
title_full |
Kint3-4 protein from human plasminogen delays Ehrlich tumor growth in mice |
title_fullStr |
Kint3-4 protein from human plasminogen delays Ehrlich tumor growth in mice |
title_full_unstemmed |
Kint3-4 protein from human plasminogen delays Ehrlich tumor growth in mice |
title_sort |
Kint3-4 protein from human plasminogen delays Ehrlich tumor growth in mice |
author |
Souza,Cristina Maria de |
author_facet |
Souza,Cristina Maria de Ferreira,Enio Ferreira,Mônica Alves Neves Diniz Andrade,Silvia Passos Santos,Ivan Carlos Pesquero,Jorge Luiz Cassali,Geovanni Dantas |
author_role |
author |
author2 |
Ferreira,Enio Ferreira,Mônica Alves Neves Diniz Andrade,Silvia Passos Santos,Ivan Carlos Pesquero,Jorge Luiz Cassali,Geovanni Dantas |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Souza,Cristina Maria de Ferreira,Enio Ferreira,Mônica Alves Neves Diniz Andrade,Silvia Passos Santos,Ivan Carlos Pesquero,Jorge Luiz Cassali,Geovanni Dantas |
dc.subject.por.fl_str_mv |
Angiogenesis Cell proliferation Angiostatin Neoplasm |
topic |
Angiogenesis Cell proliferation Angiostatin Neoplasm |
description |
INTRODUCTION AND OBJECTIVE: Kint3-4 protein, originated from a genetic recombination of K1-3 and K1-4 human plasminogen segments, is recognized for its antiangiogenic and anti-inflammatory potential. This study aimed to evaluate the effect of Kint3-4 protein on tumor development in Swiss mice previously inoculated with Ehrlich tumor cells. METHODS: The protein fragment was obtained from Pichia pastoris cloning and transformation. After tumor cell inoculation three different protocols were used to assess tumor growth: beginning (0-6 days), peak (0-12 days) and after peak (0-18 days). We analyzed tumor growth, histomorphological characteristics and immunohistochemistry by use of CDC47 (cellular proliferation marker) and CD31 (blood vessel marker). RESULTS: Animals treated with Kint3-4 protein (150 µg/kg/48 h) showed lower tumor growth in all protocols. Based on histological assessment, inflammation and tumor areas were also reduced. Moreover, both the lowest rate of tumor cell proliferation and low microvessel density were observed in animals treated with Kint3-4 protein compared with the untreated control group. CONCLUSION: The effect of Kint3-4 recombinant protein on tumor angiogenesis and control of malignant cell proliferation enhances the prospects of its use in clinical and antiangiogenic treatment. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-08-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1676-24442011000400012 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1676-24442011000400012 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1676-24442011000400012 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Patologia Clínica |
publisher.none.fl_str_mv |
Sociedade Brasileira de Patologia Clínica |
dc.source.none.fl_str_mv |
Jornal Brasileiro de Patologia e Medicina Laboratorial v.47 n.4 2011 reponame:Jornal Brasileiro de Patologia e Medicina Laboratorial (Online) instname:Sociedade Brasileira de Patologia (SBP) instacron:SBP |
instname_str |
Sociedade Brasileira de Patologia (SBP) |
instacron_str |
SBP |
institution |
SBP |
reponame_str |
Jornal Brasileiro de Patologia e Medicina Laboratorial (Online) |
collection |
Jornal Brasileiro de Patologia e Medicina Laboratorial (Online) |
repository.name.fl_str_mv |
Jornal Brasileiro de Patologia e Medicina Laboratorial (Online) - Sociedade Brasileira de Patologia (SBP) |
repository.mail.fl_str_mv |
||jbpml@sbpc.org.br |
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1752122295367761920 |