Gallic acid as the major antioxidant in pequi (Caryocar brasiliense Camb.) fruit peel
Autor(a) principal: | |
---|---|
Data de Publicação: | 2015 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista brasileira de plantas medicinais (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-05722015000400592 |
Resumo: | ABSTRACTIn this study, ethanol-water extracts of pequi fruit peel were fractionated in order to identify and quantify the major antioxidant present in it. The fractions were subjected to liquid-liquid phase extraction and silica-gel column chromatography, and antioxidant activity was monitored using the 2,2-diphenyl-1-picrylhydrazyl radical-scavenging assay. The purity of the fractions was evaluated using thin-layer chromatography and high-performance liquid chromatography (HPLC). The substance with antioxidant property was identified through the analysis in a liquid chromatography-mass spectroscopy fragmentation and was quantified using HPLC. After the Silica-gel fractionation, it was identified a fraction with high antioxidant activity and purity, which contained gallic acid as the main compound. The gallic acid was found at the amount of 26.54 ± 1.13 mg/g of the dry mass of the pequi fruit peel. Because the quantifications were performed using crude ethanol-water extract, it was suspected that gallic acid was present in a free form. Thus, pequi fruit peel may serve as an attractive alternative of feedstock for natural antioxidant production. Moreover, the results obtained in this study emphasize the value of the pequi plant, and suggests improved opportunities for families that use this fruit`s products. |
id |
SBPM-1_2ec91b91ea19f93759032b7a145fd1ef |
---|---|
oai_identifier_str |
oai:scielo:S1516-05722015000400592 |
network_acronym_str |
SBPM-1 |
network_name_str |
Revista brasileira de plantas medicinais (Online) |
repository_id_str |
|
spelling |
Gallic acid as the major antioxidant in pequi (Caryocar brasiliense Camb.) fruit peelplant extractsfractionationnatural productsantioxidantsABSTRACTIn this study, ethanol-water extracts of pequi fruit peel were fractionated in order to identify and quantify the major antioxidant present in it. The fractions were subjected to liquid-liquid phase extraction and silica-gel column chromatography, and antioxidant activity was monitored using the 2,2-diphenyl-1-picrylhydrazyl radical-scavenging assay. The purity of the fractions was evaluated using thin-layer chromatography and high-performance liquid chromatography (HPLC). The substance with antioxidant property was identified through the analysis in a liquid chromatography-mass spectroscopy fragmentation and was quantified using HPLC. After the Silica-gel fractionation, it was identified a fraction with high antioxidant activity and purity, which contained gallic acid as the main compound. The gallic acid was found at the amount of 26.54 ± 1.13 mg/g of the dry mass of the pequi fruit peel. Because the quantifications were performed using crude ethanol-water extract, it was suspected that gallic acid was present in a free form. Thus, pequi fruit peel may serve as an attractive alternative of feedstock for natural antioxidant production. Moreover, the results obtained in this study emphasize the value of the pequi plant, and suggests improved opportunities for families that use this fruit`s products.Sociedade Brasileira de Plantas Medicinais2015-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-05722015000400592Revista Brasileira de Plantas Medicinais v.17 n.4 2015reponame:Revista brasileira de plantas medicinais (Online)instname:Universidade Estadual Paulista (UNESP)instacron:SBPM10.1590/1983-084X/14_062info:eu-repo/semantics/openAccessROCHA,L. B.MELO,A. M.PAULA,S. L. A.NOBRE,S. A. M.ABREU,I. N.eng2015-10-27T00:00:00Zoai:scielo:S1516-05722015000400592Revistahttp://www.scielo.br/scielo.php?script=sci_serial&pid=1516-0572&lng=en&nrm=isoPUBhttps://old.scielo.br/oai/scielo-oai.php||rbpm.sbpm@gmail.com1983-084X1516-0572opendoar:2015-10-27T00:00Revista brasileira de plantas medicinais (Online) - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Gallic acid as the major antioxidant in pequi (Caryocar brasiliense Camb.) fruit peel |
title |
Gallic acid as the major antioxidant in pequi (Caryocar brasiliense Camb.) fruit peel |
spellingShingle |
Gallic acid as the major antioxidant in pequi (Caryocar brasiliense Camb.) fruit peel ROCHA,L. B. plant extracts fractionation natural products antioxidants |
title_short |
Gallic acid as the major antioxidant in pequi (Caryocar brasiliense Camb.) fruit peel |
title_full |
Gallic acid as the major antioxidant in pequi (Caryocar brasiliense Camb.) fruit peel |
title_fullStr |
Gallic acid as the major antioxidant in pequi (Caryocar brasiliense Camb.) fruit peel |
title_full_unstemmed |
Gallic acid as the major antioxidant in pequi (Caryocar brasiliense Camb.) fruit peel |
title_sort |
Gallic acid as the major antioxidant in pequi (Caryocar brasiliense Camb.) fruit peel |
author |
ROCHA,L. B. |
author_facet |
ROCHA,L. B. MELO,A. M. PAULA,S. L. A. NOBRE,S. A. M. ABREU,I. N. |
author_role |
author |
author2 |
MELO,A. M. PAULA,S. L. A. NOBRE,S. A. M. ABREU,I. N. |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
ROCHA,L. B. MELO,A. M. PAULA,S. L. A. NOBRE,S. A. M. ABREU,I. N. |
dc.subject.por.fl_str_mv |
plant extracts fractionation natural products antioxidants |
topic |
plant extracts fractionation natural products antioxidants |
description |
ABSTRACTIn this study, ethanol-water extracts of pequi fruit peel were fractionated in order to identify and quantify the major antioxidant present in it. The fractions were subjected to liquid-liquid phase extraction and silica-gel column chromatography, and antioxidant activity was monitored using the 2,2-diphenyl-1-picrylhydrazyl radical-scavenging assay. The purity of the fractions was evaluated using thin-layer chromatography and high-performance liquid chromatography (HPLC). The substance with antioxidant property was identified through the analysis in a liquid chromatography-mass spectroscopy fragmentation and was quantified using HPLC. After the Silica-gel fractionation, it was identified a fraction with high antioxidant activity and purity, which contained gallic acid as the main compound. The gallic acid was found at the amount of 26.54 ± 1.13 mg/g of the dry mass of the pequi fruit peel. Because the quantifications were performed using crude ethanol-water extract, it was suspected that gallic acid was present in a free form. Thus, pequi fruit peel may serve as an attractive alternative of feedstock for natural antioxidant production. Moreover, the results obtained in this study emphasize the value of the pequi plant, and suggests improved opportunities for families that use this fruit`s products. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-05722015000400592 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-05722015000400592 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1983-084X/14_062 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Plantas Medicinais |
publisher.none.fl_str_mv |
Sociedade Brasileira de Plantas Medicinais |
dc.source.none.fl_str_mv |
Revista Brasileira de Plantas Medicinais v.17 n.4 2015 reponame:Revista brasileira de plantas medicinais (Online) instname:Universidade Estadual Paulista (UNESP) instacron:SBPM |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
SBPM |
institution |
SBPM |
reponame_str |
Revista brasileira de plantas medicinais (Online) |
collection |
Revista brasileira de plantas medicinais (Online) |
repository.name.fl_str_mv |
Revista brasileira de plantas medicinais (Online) - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
||rbpm.sbpm@gmail.com |
_version_ |
1750318029354303488 |