Ethanolic extract of Casearia sylvestris Sw exhibitsin vitro antioxidant and antimicrobial activities andin vivo hypolipidemic effect in rats
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista brasileira de plantas medicinais (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-05722015000200305 |
Resumo: | ABSTRACT:The Casearia sylvestris Sw (Flacourtiaceae) is a shrub that occurs in forests of Southern Brazil; its leaves are widely used in folk medicine as a depurative, analgesic, anti-inflammatory and antiulcerogenic agent. The objective of this study was to perform the phytochemical description and to evaluate the pharmacological activities (antimicrobial, antifungal, antioxidant and toxicity) of the ethanolic extract (EE) of C. sylvestris Sw. In addition, we also evaluated the effect of the EE ofC. sylvestris Sw on the glucose levels and lipid profile in blood serum of rats submitted to a model of streptozotocin-induced diabetes. Material and Methods: In vitro assay: the detection of chemical groups was done through chemical reactions with the development of color or precipitate and by chromatographic profile; the antioxidant activity was measured by the method of reduction of DPPH free radical (2,2-diphenyl-1-picrylhydrazyl); the Minimum Inhibitory Concentration was evaluated by the broth microdilution method, and the Minimum Bactericide Concentration and the Minimum Fungicide Concentration were performed in Petri dishes; the cytotoxic activity was measured by the Artemia salina test. In vivo assay: diabetic and non-diabetic rats were treated with EE of C. sylvestris Sw (300 mg/kg) for 45 days, and the glycaemia and lipid profile were analyzed. Results: The EE showed a Lethal Dose50 of 724.76 μg.mL-1 and important antioxidant, fungicide and fungistatic activities. The EE showed better antimicrobial activity regarding the microorganismsStaphylococcus aureus, Escherichia coli andSalmonella setubal. Conclusion: The EE of C. sylvestris Sw produces a significant decrease in triglycerides, total cholesterol and VLDL levels without any significant alteration in the glycaemia. The EE of C. sylvestris Sw presents antioxidant and antimicrobial activities and it exhibits a potent hypolipidemic effect. |
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Ethanolic extract of Casearia sylvestris Sw exhibitsin vitro antioxidant and antimicrobial activities andin vivo hypolipidemic effect in ratsantioxidant activityantimicrobial activitydiabetesglycaemialipid profileABSTRACT:The Casearia sylvestris Sw (Flacourtiaceae) is a shrub that occurs in forests of Southern Brazil; its leaves are widely used in folk medicine as a depurative, analgesic, anti-inflammatory and antiulcerogenic agent. The objective of this study was to perform the phytochemical description and to evaluate the pharmacological activities (antimicrobial, antifungal, antioxidant and toxicity) of the ethanolic extract (EE) of C. sylvestris Sw. In addition, we also evaluated the effect of the EE ofC. sylvestris Sw on the glucose levels and lipid profile in blood serum of rats submitted to a model of streptozotocin-induced diabetes. Material and Methods: In vitro assay: the detection of chemical groups was done through chemical reactions with the development of color or precipitate and by chromatographic profile; the antioxidant activity was measured by the method of reduction of DPPH free radical (2,2-diphenyl-1-picrylhydrazyl); the Minimum Inhibitory Concentration was evaluated by the broth microdilution method, and the Minimum Bactericide Concentration and the Minimum Fungicide Concentration were performed in Petri dishes; the cytotoxic activity was measured by the Artemia salina test. In vivo assay: diabetic and non-diabetic rats were treated with EE of C. sylvestris Sw (300 mg/kg) for 45 days, and the glycaemia and lipid profile were analyzed. Results: The EE showed a Lethal Dose50 of 724.76 μg.mL-1 and important antioxidant, fungicide and fungistatic activities. The EE showed better antimicrobial activity regarding the microorganismsStaphylococcus aureus, Escherichia coli andSalmonella setubal. Conclusion: The EE of C. sylvestris Sw produces a significant decrease in triglycerides, total cholesterol and VLDL levels without any significant alteration in the glycaemia. The EE of C. sylvestris Sw presents antioxidant and antimicrobial activities and it exhibits a potent hypolipidemic effect.Sociedade Brasileira de Plantas Medicinais2015-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-05722015000200305Revista Brasileira de Plantas Medicinais v.17 n.2 2015reponame:Revista brasileira de plantas medicinais (Online)instname:Universidade Estadual Paulista (UNESP)instacron:SBPM10.1590/1983-084X/13_074info:eu-repo/semantics/openAccessESPINOSA,J.MEDEIROS,L.F.SOUZA,A.GÜNTZEL,A.R.C.RÜCKER,B.CASALI,E.A.ETHUR,E.M.WINK,M.R.TORRES,I.L.S.eng2015-09-10T00:00:00Zoai:scielo:S1516-05722015000200305Revistahttp://www.scielo.br/scielo.php?script=sci_serial&pid=1516-0572&lng=en&nrm=isoPUBhttps://old.scielo.br/oai/scielo-oai.php||rbpm.sbpm@gmail.com1983-084X1516-0572opendoar:2015-09-10T00:00Revista brasileira de plantas medicinais (Online) - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Ethanolic extract of Casearia sylvestris Sw exhibitsin vitro antioxidant and antimicrobial activities andin vivo hypolipidemic effect in rats |
title |
Ethanolic extract of Casearia sylvestris Sw exhibitsin vitro antioxidant and antimicrobial activities andin vivo hypolipidemic effect in rats |
spellingShingle |
Ethanolic extract of Casearia sylvestris Sw exhibitsin vitro antioxidant and antimicrobial activities andin vivo hypolipidemic effect in rats ESPINOSA,J. antioxidant activity antimicrobial activity diabetes glycaemia lipid profile |
title_short |
Ethanolic extract of Casearia sylvestris Sw exhibitsin vitro antioxidant and antimicrobial activities andin vivo hypolipidemic effect in rats |
title_full |
Ethanolic extract of Casearia sylvestris Sw exhibitsin vitro antioxidant and antimicrobial activities andin vivo hypolipidemic effect in rats |
title_fullStr |
Ethanolic extract of Casearia sylvestris Sw exhibitsin vitro antioxidant and antimicrobial activities andin vivo hypolipidemic effect in rats |
title_full_unstemmed |
Ethanolic extract of Casearia sylvestris Sw exhibitsin vitro antioxidant and antimicrobial activities andin vivo hypolipidemic effect in rats |
title_sort |
Ethanolic extract of Casearia sylvestris Sw exhibitsin vitro antioxidant and antimicrobial activities andin vivo hypolipidemic effect in rats |
author |
ESPINOSA,J. |
author_facet |
ESPINOSA,J. MEDEIROS,L.F. SOUZA,A. GÜNTZEL,A.R.C. RÜCKER,B. CASALI,E.A. ETHUR,E.M. WINK,M.R. TORRES,I.L.S. |
author_role |
author |
author2 |
MEDEIROS,L.F. SOUZA,A. GÜNTZEL,A.R.C. RÜCKER,B. CASALI,E.A. ETHUR,E.M. WINK,M.R. TORRES,I.L.S. |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
ESPINOSA,J. MEDEIROS,L.F. SOUZA,A. GÜNTZEL,A.R.C. RÜCKER,B. CASALI,E.A. ETHUR,E.M. WINK,M.R. TORRES,I.L.S. |
dc.subject.por.fl_str_mv |
antioxidant activity antimicrobial activity diabetes glycaemia lipid profile |
topic |
antioxidant activity antimicrobial activity diabetes glycaemia lipid profile |
description |
ABSTRACT:The Casearia sylvestris Sw (Flacourtiaceae) is a shrub that occurs in forests of Southern Brazil; its leaves are widely used in folk medicine as a depurative, analgesic, anti-inflammatory and antiulcerogenic agent. The objective of this study was to perform the phytochemical description and to evaluate the pharmacological activities (antimicrobial, antifungal, antioxidant and toxicity) of the ethanolic extract (EE) of C. sylvestris Sw. In addition, we also evaluated the effect of the EE ofC. sylvestris Sw on the glucose levels and lipid profile in blood serum of rats submitted to a model of streptozotocin-induced diabetes. Material and Methods: In vitro assay: the detection of chemical groups was done through chemical reactions with the development of color or precipitate and by chromatographic profile; the antioxidant activity was measured by the method of reduction of DPPH free radical (2,2-diphenyl-1-picrylhydrazyl); the Minimum Inhibitory Concentration was evaluated by the broth microdilution method, and the Minimum Bactericide Concentration and the Minimum Fungicide Concentration were performed in Petri dishes; the cytotoxic activity was measured by the Artemia salina test. In vivo assay: diabetic and non-diabetic rats were treated with EE of C. sylvestris Sw (300 mg/kg) for 45 days, and the glycaemia and lipid profile were analyzed. Results: The EE showed a Lethal Dose50 of 724.76 μg.mL-1 and important antioxidant, fungicide and fungistatic activities. The EE showed better antimicrobial activity regarding the microorganismsStaphylococcus aureus, Escherichia coli andSalmonella setubal. Conclusion: The EE of C. sylvestris Sw produces a significant decrease in triglycerides, total cholesterol and VLDL levels without any significant alteration in the glycaemia. The EE of C. sylvestris Sw presents antioxidant and antimicrobial activities and it exhibits a potent hypolipidemic effect. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-05722015000200305 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-05722015000200305 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1983-084X/13_074 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Plantas Medicinais |
publisher.none.fl_str_mv |
Sociedade Brasileira de Plantas Medicinais |
dc.source.none.fl_str_mv |
Revista Brasileira de Plantas Medicinais v.17 n.2 2015 reponame:Revista brasileira de plantas medicinais (Online) instname:Universidade Estadual Paulista (UNESP) instacron:SBPM |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
SBPM |
institution |
SBPM |
reponame_str |
Revista brasileira de plantas medicinais (Online) |
collection |
Revista brasileira de plantas medicinais (Online) |
repository.name.fl_str_mv |
Revista brasileira de plantas medicinais (Online) - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
||rbpm.sbpm@gmail.com |
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1750318029024002048 |