Titanium dioxide nanotubes added to glass ionomer cements affect S. mutans viability and mechanisms of virulence

Detalhes bibliográficos
Autor(a) principal: ARAÚJO,Isaac Jordão de Souza
Data de Publicação: 2021
Outros Autores: RICARDO,Mariana Gallante, GOMES,Orisson Ponce, GIOVANI,Priscila Alves, PUPPIN-RONTANI,Júlia, PECORARI,Vanessa Arias, MARTINEZ,Elizabeth Ferreira, NAPIMOGA,Marcelo Henrique, NOCITI JUNIOR,Francisco Humberto, PUPPIN-RONTANI,Regina Maria, LISBOA-FILHO,Paulo Noronha, KANTOVITZ,Kamila Rosamilia
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Oral Research
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242021000100253
Resumo: Abstract This in vitro study evaluated the impact of TiO2 nanotubes (n-TiO2) incorporated into glass ionomer cement (GIC) on Streptococcus mutans (S. mutans) characteristics at cellular and molecular levels. n-TiO2, synthesized by the alkaline method (20 nm in size), was added to Ketac Molar EasyMix® at 0%, 3%, 5%, and 7% by weight. S. mutans strains were cultured on GIC disks with addition or not of n-TiO2 for 1, 3, and 7 days and the following parameters were assessed: inhibition halo (mm) (n=3/group); cell viability (live/dead) (n=5/group); cell morphology (SEM) (n=3/group); and gene expression by real-time PCR (vicR, covR, gtfB, gtfC, and gtfD) (n=6/group). The data were analyzed by the Kruskal-Wallis test, repeated-measures ANOVA or two-way ANOVA, and Tukey’s and Dunn’s post-hoc tests (α=0.05). The agar diffusion test showed a higher antibacterial property for 5% n-TiO2 compared with 3% and 7% (p<0.05) with no effect of time (1, 3, and 7 days). The cell number was significantly affected by all n-TiO2 groups, while viability was mostly affected by 3% and 5% n-TiO2, which also affected cell morphology and organization. Real-time PCR demonstrated that n-TiO2 reduced the expression of covR when compared with GIC with no n-TiO2 (p<0.05), with no effect of time, except for 3% n-TiO2 on vicR expression. Within-group and between-group analyses revealed n-TiO2 did not affect mRNA levels of gtfB, gtfC, and gtfD (p>0.05). Incorporation of n-TiO2 at 3% and 5% potentially affected S. mutans viability and the expression of key genes for bacterial survival and growth, improving the anticariogenic properties of GIC.
id SBPQO-1_174c1432d6ac601d90cfa2e378fd8eb6
oai_identifier_str oai:scielo:S1806-83242021000100253
network_acronym_str SBPQO-1
network_name_str Brazilian Oral Research
repository_id_str
spelling Titanium dioxide nanotubes added to glass ionomer cements affect S. mutans viability and mechanisms of virulenceNanotechnologyGlass Ionomer CementsTitaniumGene ExpressionAbstract This in vitro study evaluated the impact of TiO2 nanotubes (n-TiO2) incorporated into glass ionomer cement (GIC) on Streptococcus mutans (S. mutans) characteristics at cellular and molecular levels. n-TiO2, synthesized by the alkaline method (20 nm in size), was added to Ketac Molar EasyMix® at 0%, 3%, 5%, and 7% by weight. S. mutans strains were cultured on GIC disks with addition or not of n-TiO2 for 1, 3, and 7 days and the following parameters were assessed: inhibition halo (mm) (n=3/group); cell viability (live/dead) (n=5/group); cell morphology (SEM) (n=3/group); and gene expression by real-time PCR (vicR, covR, gtfB, gtfC, and gtfD) (n=6/group). The data were analyzed by the Kruskal-Wallis test, repeated-measures ANOVA or two-way ANOVA, and Tukey’s and Dunn’s post-hoc tests (α=0.05). The agar diffusion test showed a higher antibacterial property for 5% n-TiO2 compared with 3% and 7% (p<0.05) with no effect of time (1, 3, and 7 days). The cell number was significantly affected by all n-TiO2 groups, while viability was mostly affected by 3% and 5% n-TiO2, which also affected cell morphology and organization. Real-time PCR demonstrated that n-TiO2 reduced the expression of covR when compared with GIC with no n-TiO2 (p<0.05), with no effect of time, except for 3% n-TiO2 on vicR expression. Within-group and between-group analyses revealed n-TiO2 did not affect mRNA levels of gtfB, gtfC, and gtfD (p>0.05). Incorporation of n-TiO2 at 3% and 5% potentially affected S. mutans viability and the expression of key genes for bacterial survival and growth, improving the anticariogenic properties of GIC.Sociedade Brasileira de Pesquisa Odontológica - SBPqO2021-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242021000100253Brazilian Oral Research v.35 2021reponame:Brazilian Oral Researchinstname:Sociedade Brasileira de Pesquisa Odontológica (SBPqO)instacron:SBPQO10.1590/1807-3107bor-2021.vol35.0062info:eu-repo/semantics/openAccessARAÚJO,Isaac Jordão de SouzaRICARDO,Mariana GallanteGOMES,Orisson PonceGIOVANI,Priscila AlvesPUPPIN-RONTANI,JúliaPECORARI,Vanessa AriasMARTINEZ,Elizabeth FerreiraNAPIMOGA,Marcelo HenriqueNOCITI JUNIOR,Francisco HumbertoPUPPIN-RONTANI,Regina MariaLISBOA-FILHO,Paulo NoronhaKANTOVITZ,Kamila Rosamiliaeng2021-06-11T00:00:00Zoai:scielo:S1806-83242021000100253Revistahttps://www.scielo.br/j/bor/https://old.scielo.br/oai/scielo-oai.phppob@edu.usp.br||bor@sbpqo.org.br1807-31071806-8324opendoar:2021-06-11T00:00Brazilian Oral Research - Sociedade Brasileira de Pesquisa Odontológica (SBPqO)false
dc.title.none.fl_str_mv Titanium dioxide nanotubes added to glass ionomer cements affect S. mutans viability and mechanisms of virulence
title Titanium dioxide nanotubes added to glass ionomer cements affect S. mutans viability and mechanisms of virulence
spellingShingle Titanium dioxide nanotubes added to glass ionomer cements affect S. mutans viability and mechanisms of virulence
ARAÚJO,Isaac Jordão de Souza
Nanotechnology
Glass Ionomer Cements
Titanium
Gene Expression
title_short Titanium dioxide nanotubes added to glass ionomer cements affect S. mutans viability and mechanisms of virulence
title_full Titanium dioxide nanotubes added to glass ionomer cements affect S. mutans viability and mechanisms of virulence
title_fullStr Titanium dioxide nanotubes added to glass ionomer cements affect S. mutans viability and mechanisms of virulence
title_full_unstemmed Titanium dioxide nanotubes added to glass ionomer cements affect S. mutans viability and mechanisms of virulence
title_sort Titanium dioxide nanotubes added to glass ionomer cements affect S. mutans viability and mechanisms of virulence
author ARAÚJO,Isaac Jordão de Souza
author_facet ARAÚJO,Isaac Jordão de Souza
RICARDO,Mariana Gallante
GOMES,Orisson Ponce
GIOVANI,Priscila Alves
PUPPIN-RONTANI,Júlia
PECORARI,Vanessa Arias
MARTINEZ,Elizabeth Ferreira
NAPIMOGA,Marcelo Henrique
NOCITI JUNIOR,Francisco Humberto
PUPPIN-RONTANI,Regina Maria
LISBOA-FILHO,Paulo Noronha
KANTOVITZ,Kamila Rosamilia
author_role author
author2 RICARDO,Mariana Gallante
GOMES,Orisson Ponce
GIOVANI,Priscila Alves
PUPPIN-RONTANI,Júlia
PECORARI,Vanessa Arias
MARTINEZ,Elizabeth Ferreira
NAPIMOGA,Marcelo Henrique
NOCITI JUNIOR,Francisco Humberto
PUPPIN-RONTANI,Regina Maria
LISBOA-FILHO,Paulo Noronha
KANTOVITZ,Kamila Rosamilia
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv ARAÚJO,Isaac Jordão de Souza
RICARDO,Mariana Gallante
GOMES,Orisson Ponce
GIOVANI,Priscila Alves
PUPPIN-RONTANI,Júlia
PECORARI,Vanessa Arias
MARTINEZ,Elizabeth Ferreira
NAPIMOGA,Marcelo Henrique
NOCITI JUNIOR,Francisco Humberto
PUPPIN-RONTANI,Regina Maria
LISBOA-FILHO,Paulo Noronha
KANTOVITZ,Kamila Rosamilia
dc.subject.por.fl_str_mv Nanotechnology
Glass Ionomer Cements
Titanium
Gene Expression
topic Nanotechnology
Glass Ionomer Cements
Titanium
Gene Expression
description Abstract This in vitro study evaluated the impact of TiO2 nanotubes (n-TiO2) incorporated into glass ionomer cement (GIC) on Streptococcus mutans (S. mutans) characteristics at cellular and molecular levels. n-TiO2, synthesized by the alkaline method (20 nm in size), was added to Ketac Molar EasyMix® at 0%, 3%, 5%, and 7% by weight. S. mutans strains were cultured on GIC disks with addition or not of n-TiO2 for 1, 3, and 7 days and the following parameters were assessed: inhibition halo (mm) (n=3/group); cell viability (live/dead) (n=5/group); cell morphology (SEM) (n=3/group); and gene expression by real-time PCR (vicR, covR, gtfB, gtfC, and gtfD) (n=6/group). The data were analyzed by the Kruskal-Wallis test, repeated-measures ANOVA or two-way ANOVA, and Tukey’s and Dunn’s post-hoc tests (α=0.05). The agar diffusion test showed a higher antibacterial property for 5% n-TiO2 compared with 3% and 7% (p<0.05) with no effect of time (1, 3, and 7 days). The cell number was significantly affected by all n-TiO2 groups, while viability was mostly affected by 3% and 5% n-TiO2, which also affected cell morphology and organization. Real-time PCR demonstrated that n-TiO2 reduced the expression of covR when compared with GIC with no n-TiO2 (p<0.05), with no effect of time, except for 3% n-TiO2 on vicR expression. Within-group and between-group analyses revealed n-TiO2 did not affect mRNA levels of gtfB, gtfC, and gtfD (p>0.05). Incorporation of n-TiO2 at 3% and 5% potentially affected S. mutans viability and the expression of key genes for bacterial survival and growth, improving the anticariogenic properties of GIC.
publishDate 2021
dc.date.none.fl_str_mv 2021-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242021000100253
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242021000100253
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1807-3107bor-2021.vol35.0062
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Pesquisa Odontológica - SBPqO
publisher.none.fl_str_mv Sociedade Brasileira de Pesquisa Odontológica - SBPqO
dc.source.none.fl_str_mv Brazilian Oral Research v.35 2021
reponame:Brazilian Oral Research
instname:Sociedade Brasileira de Pesquisa Odontológica (SBPqO)
instacron:SBPQO
instname_str Sociedade Brasileira de Pesquisa Odontológica (SBPqO)
instacron_str SBPQO
institution SBPQO
reponame_str Brazilian Oral Research
collection Brazilian Oral Research
repository.name.fl_str_mv Brazilian Oral Research - Sociedade Brasileira de Pesquisa Odontológica (SBPqO)
repository.mail.fl_str_mv pob@edu.usp.br||bor@sbpqo.org.br
_version_ 1750318327626989568