Antifungal, antibiofilm, and antiproliferative activities of Guapira graciliflora Mart
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Oral Research |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242018000100236 |
Resumo: | Abstract: The aim of this study was to evaluate in vitro the antifungal, antibiofilm and antiproliferative activities of the extract from the leaves of Guapira graciliflora Mart. The phytochemical characterization of the extract was performed using electrospray ionization mass spectrometry (ESI-MS). The antimicrobial activity of the extract and its fractions was evaluated using the broth microdilution method against species of Candida. The inhibition of C. albicans biofilm was evaluated based on the number of colony-forming units (CFU) and metabolic activity (MTT). The antiproliferative activity of the extract and its fraction was evaluated in the presence of human tumor and non-tumor cells, and the cytotoxicity of the extract was determined on the RAW 264.7 macrophage line – both using the sulforhodamine B method. The phytochemical characterization indicated the presence of the flavonoids rutin and kaempferol. The extract and the methanol fraction exhibited moderate antifungal activity against C. albicans, C. krusei, and C. glabrata, and strong activity against C. dubliniensis. In the biofilms at 24 and 48 hours, the concentration of 12500 µg/mL of the extract was the most effective at reducing the number of CFU s/mL (44.4% and 42.9%, respectively) and the metabolic activity of C. albicans cells (34.6% and 52%, respectively). The extract and its fractions had no antiproliferative effect on the tumor lines tested, with mean activity (log GI50) equal to or greater than 1.71 µg/mL. Macrophage cell viability remained higher than 80% for concentrations of the extract of up to 62.5 µg/mL. G. graciliflora has flavonoids in its chemical composition and demonstrates potential antifungal and antibiofilm activity, with no evidence of a significant change in the viability of human tumor and non-tumor cell lines. |
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Antifungal, antibiofilm, and antiproliferative activities of Guapira graciliflora MartPlantsMedicinalNyctaginaceaeCandidaBiofilmsAbstract: The aim of this study was to evaluate in vitro the antifungal, antibiofilm and antiproliferative activities of the extract from the leaves of Guapira graciliflora Mart. The phytochemical characterization of the extract was performed using electrospray ionization mass spectrometry (ESI-MS). The antimicrobial activity of the extract and its fractions was evaluated using the broth microdilution method against species of Candida. The inhibition of C. albicans biofilm was evaluated based on the number of colony-forming units (CFU) and metabolic activity (MTT). The antiproliferative activity of the extract and its fraction was evaluated in the presence of human tumor and non-tumor cells, and the cytotoxicity of the extract was determined on the RAW 264.7 macrophage line – both using the sulforhodamine B method. The phytochemical characterization indicated the presence of the flavonoids rutin and kaempferol. The extract and the methanol fraction exhibited moderate antifungal activity against C. albicans, C. krusei, and C. glabrata, and strong activity against C. dubliniensis. In the biofilms at 24 and 48 hours, the concentration of 12500 µg/mL of the extract was the most effective at reducing the number of CFU s/mL (44.4% and 42.9%, respectively) and the metabolic activity of C. albicans cells (34.6% and 52%, respectively). The extract and its fractions had no antiproliferative effect on the tumor lines tested, with mean activity (log GI50) equal to or greater than 1.71 µg/mL. Macrophage cell viability remained higher than 80% for concentrations of the extract of up to 62.5 µg/mL. G. graciliflora has flavonoids in its chemical composition and demonstrates potential antifungal and antibiofilm activity, with no evidence of a significant change in the viability of human tumor and non-tumor cell lines.Sociedade Brasileira de Pesquisa Odontológica - SBPqO2018-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242018000100236Brazilian Oral Research v.32 2018reponame:Brazilian Oral Researchinstname:Sociedade Brasileira de Pesquisa Odontológica (SBPqO)instacron:SBPQO10.1590/1807-3107bor-2018.vol32.0041info:eu-repo/semantics/openAccessAlmeida,Carolina Medeiros deLima,Rennaly de FreitasCosta,Tereza Karla Vieira Lopes daSousa,Ilza Maria de OliveiraCabral,Elaine CristinaBasting,Rosanna TarkanyTorre,Adriana DellaCavalcanti,Yuri WanderleyRosalen,Pedro LuizDuarte,Marta Cristina TeixeiraRuiz,Ana Lúcia Tasca GoisFoglio,Mary AnnGodoy,Gustavo PinaCosta,Edja Maria Melo de Britoeng2018-05-21T00:00:00Zoai:scielo:S1806-83242018000100236Revistahttps://www.scielo.br/j/bor/https://old.scielo.br/oai/scielo-oai.phppob@edu.usp.br||bor@sbpqo.org.br1807-31071806-8324opendoar:2018-05-21T00:00Brazilian Oral Research - Sociedade Brasileira de Pesquisa Odontológica (SBPqO)false |
dc.title.none.fl_str_mv |
Antifungal, antibiofilm, and antiproliferative activities of Guapira graciliflora Mart |
title |
Antifungal, antibiofilm, and antiproliferative activities of Guapira graciliflora Mart |
spellingShingle |
Antifungal, antibiofilm, and antiproliferative activities of Guapira graciliflora Mart Almeida,Carolina Medeiros de Plants Medicinal Nyctaginaceae Candida Biofilms |
title_short |
Antifungal, antibiofilm, and antiproliferative activities of Guapira graciliflora Mart |
title_full |
Antifungal, antibiofilm, and antiproliferative activities of Guapira graciliflora Mart |
title_fullStr |
Antifungal, antibiofilm, and antiproliferative activities of Guapira graciliflora Mart |
title_full_unstemmed |
Antifungal, antibiofilm, and antiproliferative activities of Guapira graciliflora Mart |
title_sort |
Antifungal, antibiofilm, and antiproliferative activities of Guapira graciliflora Mart |
author |
Almeida,Carolina Medeiros de |
author_facet |
Almeida,Carolina Medeiros de Lima,Rennaly de Freitas Costa,Tereza Karla Vieira Lopes da Sousa,Ilza Maria de Oliveira Cabral,Elaine Cristina Basting,Rosanna Tarkany Torre,Adriana Della Cavalcanti,Yuri Wanderley Rosalen,Pedro Luiz Duarte,Marta Cristina Teixeira Ruiz,Ana Lúcia Tasca Gois Foglio,Mary Ann Godoy,Gustavo Pina Costa,Edja Maria Melo de Brito |
author_role |
author |
author2 |
Lima,Rennaly de Freitas Costa,Tereza Karla Vieira Lopes da Sousa,Ilza Maria de Oliveira Cabral,Elaine Cristina Basting,Rosanna Tarkany Torre,Adriana Della Cavalcanti,Yuri Wanderley Rosalen,Pedro Luiz Duarte,Marta Cristina Teixeira Ruiz,Ana Lúcia Tasca Gois Foglio,Mary Ann Godoy,Gustavo Pina Costa,Edja Maria Melo de Brito |
author2_role |
author author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Almeida,Carolina Medeiros de Lima,Rennaly de Freitas Costa,Tereza Karla Vieira Lopes da Sousa,Ilza Maria de Oliveira Cabral,Elaine Cristina Basting,Rosanna Tarkany Torre,Adriana Della Cavalcanti,Yuri Wanderley Rosalen,Pedro Luiz Duarte,Marta Cristina Teixeira Ruiz,Ana Lúcia Tasca Gois Foglio,Mary Ann Godoy,Gustavo Pina Costa,Edja Maria Melo de Brito |
dc.subject.por.fl_str_mv |
Plants Medicinal Nyctaginaceae Candida Biofilms |
topic |
Plants Medicinal Nyctaginaceae Candida Biofilms |
description |
Abstract: The aim of this study was to evaluate in vitro the antifungal, antibiofilm and antiproliferative activities of the extract from the leaves of Guapira graciliflora Mart. The phytochemical characterization of the extract was performed using electrospray ionization mass spectrometry (ESI-MS). The antimicrobial activity of the extract and its fractions was evaluated using the broth microdilution method against species of Candida. The inhibition of C. albicans biofilm was evaluated based on the number of colony-forming units (CFU) and metabolic activity (MTT). The antiproliferative activity of the extract and its fraction was evaluated in the presence of human tumor and non-tumor cells, and the cytotoxicity of the extract was determined on the RAW 264.7 macrophage line – both using the sulforhodamine B method. The phytochemical characterization indicated the presence of the flavonoids rutin and kaempferol. The extract and the methanol fraction exhibited moderate antifungal activity against C. albicans, C. krusei, and C. glabrata, and strong activity against C. dubliniensis. In the biofilms at 24 and 48 hours, the concentration of 12500 µg/mL of the extract was the most effective at reducing the number of CFU s/mL (44.4% and 42.9%, respectively) and the metabolic activity of C. albicans cells (34.6% and 52%, respectively). The extract and its fractions had no antiproliferative effect on the tumor lines tested, with mean activity (log GI50) equal to or greater than 1.71 µg/mL. Macrophage cell viability remained higher than 80% for concentrations of the extract of up to 62.5 µg/mL. G. graciliflora has flavonoids in its chemical composition and demonstrates potential antifungal and antibiofilm activity, with no evidence of a significant change in the viability of human tumor and non-tumor cell lines. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242018000100236 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242018000100236 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1807-3107bor-2018.vol32.0041 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Pesquisa Odontológica - SBPqO |
publisher.none.fl_str_mv |
Sociedade Brasileira de Pesquisa Odontológica - SBPqO |
dc.source.none.fl_str_mv |
Brazilian Oral Research v.32 2018 reponame:Brazilian Oral Research instname:Sociedade Brasileira de Pesquisa Odontológica (SBPqO) instacron:SBPQO |
instname_str |
Sociedade Brasileira de Pesquisa Odontológica (SBPqO) |
instacron_str |
SBPQO |
institution |
SBPQO |
reponame_str |
Brazilian Oral Research |
collection |
Brazilian Oral Research |
repository.name.fl_str_mv |
Brazilian Oral Research - Sociedade Brasileira de Pesquisa Odontológica (SBPqO) |
repository.mail.fl_str_mv |
pob@edu.usp.br||bor@sbpqo.org.br |
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1750318325755281408 |