Isolation and characterization of a human cementocyte-like cell line, HCY-23
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Oral Research |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242019000100255 |
Resumo: | Abstract Cementum is the mineralized tissue covering the tooth root that functions in tooth attachment and post-eruptive adjustment of tooth position. It has been reported to be highly similar to bone in several respects but remains poorly understood in terms of development and regeneration. Here, we investigate whether cementocytes, the residing cells in cellular cementum, have the potential to be protagonist in cementum homeostasis, responding to endocrine signals and directing local cementum metabolism. Cells from healthy erupted human teeth were isolated using sequential collagenase/EDTA digestions, and maintained in standard cell culture conditions. A cementocyte-like cell line was cloned (HCY-23, for human cementocyte clone 23), which presented a cementocyte compatible gene expression signature, including the expression of dentin matrix protein 1 ( DMP1 ), sclerostin ( SOST ), and E11/gp38/podoplanin ( E11 ). In contrast, these cells did not express the odontoblast/dentin marker dentin sialoprotein ( DSPP ). HCY-23 cells produced mineral-like nodules in vitro under differentiation conditions, and were highly responsive to inorganic phosphate (Pi). Within the limits of the present study, it can be concluded that cementocytes are phosphate-responsive cells, and have the potential do play a key role in periodontal homeostasis and regeneration. |
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Isolation and characterization of a human cementocyte-like cell line, HCY-23Dental CementumGene ExpressionCell Culture TechniquesAbstract Cementum is the mineralized tissue covering the tooth root that functions in tooth attachment and post-eruptive adjustment of tooth position. It has been reported to be highly similar to bone in several respects but remains poorly understood in terms of development and regeneration. Here, we investigate whether cementocytes, the residing cells in cellular cementum, have the potential to be protagonist in cementum homeostasis, responding to endocrine signals and directing local cementum metabolism. Cells from healthy erupted human teeth were isolated using sequential collagenase/EDTA digestions, and maintained in standard cell culture conditions. A cementocyte-like cell line was cloned (HCY-23, for human cementocyte clone 23), which presented a cementocyte compatible gene expression signature, including the expression of dentin matrix protein 1 ( DMP1 ), sclerostin ( SOST ), and E11/gp38/podoplanin ( E11 ). In contrast, these cells did not express the odontoblast/dentin marker dentin sialoprotein ( DSPP ). HCY-23 cells produced mineral-like nodules in vitro under differentiation conditions, and were highly responsive to inorganic phosphate (Pi). Within the limits of the present study, it can be concluded that cementocytes are phosphate-responsive cells, and have the potential do play a key role in periodontal homeostasis and regeneration.Sociedade Brasileira de Pesquisa Odontológica - SBPqO2019-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242019000100255Brazilian Oral Research v.33 2019reponame:Brazilian Oral Researchinstname:Sociedade Brasileira de Pesquisa Odontológica (SBPqO)instacron:SBPQO10.1590/1807-3107bor-2019.vol33.0058info:eu-repo/semantics/openAccessALMEIDA,Amanda Bandeira deSANTOS,Elis Janaína Lira dosABUNA,Gabriel FloresRIBEIRO,Cristiane SalmonCASATI,Márcio ZaffalonRUIZ,Karina Gonzales SilvérioNOCITI JUNIOR,Francisco Humbertoeng2019-08-13T00:00:00Zoai:scielo:S1806-83242019000100255Revistahttps://www.scielo.br/j/bor/https://old.scielo.br/oai/scielo-oai.phppob@edu.usp.br||bor@sbpqo.org.br1807-31071806-8324opendoar:2019-08-13T00:00Brazilian Oral Research - Sociedade Brasileira de Pesquisa Odontológica (SBPqO)false |
dc.title.none.fl_str_mv |
Isolation and characterization of a human cementocyte-like cell line, HCY-23 |
title |
Isolation and characterization of a human cementocyte-like cell line, HCY-23 |
spellingShingle |
Isolation and characterization of a human cementocyte-like cell line, HCY-23 ALMEIDA,Amanda Bandeira de Dental Cementum Gene Expression Cell Culture Techniques |
title_short |
Isolation and characterization of a human cementocyte-like cell line, HCY-23 |
title_full |
Isolation and characterization of a human cementocyte-like cell line, HCY-23 |
title_fullStr |
Isolation and characterization of a human cementocyte-like cell line, HCY-23 |
title_full_unstemmed |
Isolation and characterization of a human cementocyte-like cell line, HCY-23 |
title_sort |
Isolation and characterization of a human cementocyte-like cell line, HCY-23 |
author |
ALMEIDA,Amanda Bandeira de |
author_facet |
ALMEIDA,Amanda Bandeira de SANTOS,Elis Janaína Lira dos ABUNA,Gabriel Flores RIBEIRO,Cristiane Salmon CASATI,Márcio Zaffalon RUIZ,Karina Gonzales Silvério NOCITI JUNIOR,Francisco Humberto |
author_role |
author |
author2 |
SANTOS,Elis Janaína Lira dos ABUNA,Gabriel Flores RIBEIRO,Cristiane Salmon CASATI,Márcio Zaffalon RUIZ,Karina Gonzales Silvério NOCITI JUNIOR,Francisco Humberto |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
ALMEIDA,Amanda Bandeira de SANTOS,Elis Janaína Lira dos ABUNA,Gabriel Flores RIBEIRO,Cristiane Salmon CASATI,Márcio Zaffalon RUIZ,Karina Gonzales Silvério NOCITI JUNIOR,Francisco Humberto |
dc.subject.por.fl_str_mv |
Dental Cementum Gene Expression Cell Culture Techniques |
topic |
Dental Cementum Gene Expression Cell Culture Techniques |
description |
Abstract Cementum is the mineralized tissue covering the tooth root that functions in tooth attachment and post-eruptive adjustment of tooth position. It has been reported to be highly similar to bone in several respects but remains poorly understood in terms of development and regeneration. Here, we investigate whether cementocytes, the residing cells in cellular cementum, have the potential to be protagonist in cementum homeostasis, responding to endocrine signals and directing local cementum metabolism. Cells from healthy erupted human teeth were isolated using sequential collagenase/EDTA digestions, and maintained in standard cell culture conditions. A cementocyte-like cell line was cloned (HCY-23, for human cementocyte clone 23), which presented a cementocyte compatible gene expression signature, including the expression of dentin matrix protein 1 ( DMP1 ), sclerostin ( SOST ), and E11/gp38/podoplanin ( E11 ). In contrast, these cells did not express the odontoblast/dentin marker dentin sialoprotein ( DSPP ). HCY-23 cells produced mineral-like nodules in vitro under differentiation conditions, and were highly responsive to inorganic phosphate (Pi). Within the limits of the present study, it can be concluded that cementocytes are phosphate-responsive cells, and have the potential do play a key role in periodontal homeostasis and regeneration. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242019000100255 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242019000100255 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1807-3107bor-2019.vol33.0058 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Pesquisa Odontológica - SBPqO |
publisher.none.fl_str_mv |
Sociedade Brasileira de Pesquisa Odontológica - SBPqO |
dc.source.none.fl_str_mv |
Brazilian Oral Research v.33 2019 reponame:Brazilian Oral Research instname:Sociedade Brasileira de Pesquisa Odontológica (SBPqO) instacron:SBPQO |
instname_str |
Sociedade Brasileira de Pesquisa Odontológica (SBPqO) |
instacron_str |
SBPQO |
institution |
SBPQO |
reponame_str |
Brazilian Oral Research |
collection |
Brazilian Oral Research |
repository.name.fl_str_mv |
Brazilian Oral Research - Sociedade Brasileira de Pesquisa Odontológica (SBPqO) |
repository.mail.fl_str_mv |
pob@edu.usp.br||bor@sbpqo.org.br |
_version_ |
1750318326588899328 |