Effect of cadmium on the floor of the mouth on rats during lactation
Autor(a) principal: | |
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Data de Publicação: | 2004 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Oral Research |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242004000200003 |
Resumo: | Cadmium (Cd) present in the air, drinking water and food has the potential to affect the health of people, mainly those who live in highly industrialized regions. Cd affects placental function, may cross the placental barrier and directly modify fetal development. It is also excreted into milk. The body is particularly susceptible to Cd exposure during perinatal period. The effect on rat oral epithelium (floor of the mouth) after continuous exposure to drinking water containing low levels of Cd during lactation was studied. Female rats were supplied with ad libitum drinking water containing 300 mg/l of CdCl2 throughout the whole lactation period. Control animals received a similar volume of water without Cd. Lactating rats (21 day-old) were killed by lethal dose of anesthetic. The heads were retrieved, fixed in "alfac" solution (alcohol, acetic acid and formaldehyde) for 24 h, serially sectioned in frontal plane, at the level of the first molars. The 6 µm sections were then stained with hematoxylin and eosin. Nuclear epithelium parameters were estimated, as well as cytoplasm and cell volume, nucleus/cytoplasm ratio, numeric and surface densities, and epithelial thickness. Mean body weight was 34.86 g for the control group and 18.56 g for the Cd-treated group. Histologically, the floor of the mouth epithelium was thinner in the treated group, with smaller and more numerous cells. In this experiment, Cd induced epithelial hypotrophy, indicating a direct action in oral mucosa cells, besides retarded development of the pups. |
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Effect of cadmium on the floor of the mouth on rats during lactationMouth mucosaMicroscopy, polarizationCadmiumMorphometryStereologyCadmium (Cd) present in the air, drinking water and food has the potential to affect the health of people, mainly those who live in highly industrialized regions. Cd affects placental function, may cross the placental barrier and directly modify fetal development. It is also excreted into milk. The body is particularly susceptible to Cd exposure during perinatal period. The effect on rat oral epithelium (floor of the mouth) after continuous exposure to drinking water containing low levels of Cd during lactation was studied. Female rats were supplied with ad libitum drinking water containing 300 mg/l of CdCl2 throughout the whole lactation period. Control animals received a similar volume of water without Cd. Lactating rats (21 day-old) were killed by lethal dose of anesthetic. The heads were retrieved, fixed in "alfac" solution (alcohol, acetic acid and formaldehyde) for 24 h, serially sectioned in frontal plane, at the level of the first molars. The 6 µm sections were then stained with hematoxylin and eosin. Nuclear epithelium parameters were estimated, as well as cytoplasm and cell volume, nucleus/cytoplasm ratio, numeric and surface densities, and epithelial thickness. Mean body weight was 34.86 g for the control group and 18.56 g for the Cd-treated group. Histologically, the floor of the mouth epithelium was thinner in the treated group, with smaller and more numerous cells. In this experiment, Cd induced epithelial hypotrophy, indicating a direct action in oral mucosa cells, besides retarded development of the pups.Sociedade Brasileira de Pesquisa Odontológica - SBPqO2004-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242004000200003Brazilian Oral Research v.18 n.2 2004reponame:Brazilian Oral Researchinstname:Sociedade Brasileira de Pesquisa Odontológica (SBPqO)instacron:SBPQO10.1590/S1806-83242004000200003info:eu-repo/semantics/openAccessPicoli,Lara CristinaWatanabe,Ii-SeiLopes,Ruberval ArmandoSala,Miguel AngelPicoli,Fábioeng2004-09-29T00:00:00Zoai:scielo:S1806-83242004000200003Revistahttps://www.scielo.br/j/bor/https://old.scielo.br/oai/scielo-oai.phppob@edu.usp.br||bor@sbpqo.org.br1807-31071806-8324opendoar:2004-09-29T00:00Brazilian Oral Research - Sociedade Brasileira de Pesquisa Odontológica (SBPqO)false |
dc.title.none.fl_str_mv |
Effect of cadmium on the floor of the mouth on rats during lactation |
title |
Effect of cadmium on the floor of the mouth on rats during lactation |
spellingShingle |
Effect of cadmium on the floor of the mouth on rats during lactation Picoli,Lara Cristina Mouth mucosa Microscopy, polarization Cadmium Morphometry Stereology |
title_short |
Effect of cadmium on the floor of the mouth on rats during lactation |
title_full |
Effect of cadmium on the floor of the mouth on rats during lactation |
title_fullStr |
Effect of cadmium on the floor of the mouth on rats during lactation |
title_full_unstemmed |
Effect of cadmium on the floor of the mouth on rats during lactation |
title_sort |
Effect of cadmium on the floor of the mouth on rats during lactation |
author |
Picoli,Lara Cristina |
author_facet |
Picoli,Lara Cristina Watanabe,Ii-Sei Lopes,Ruberval Armando Sala,Miguel Angel Picoli,Fábio |
author_role |
author |
author2 |
Watanabe,Ii-Sei Lopes,Ruberval Armando Sala,Miguel Angel Picoli,Fábio |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Picoli,Lara Cristina Watanabe,Ii-Sei Lopes,Ruberval Armando Sala,Miguel Angel Picoli,Fábio |
dc.subject.por.fl_str_mv |
Mouth mucosa Microscopy, polarization Cadmium Morphometry Stereology |
topic |
Mouth mucosa Microscopy, polarization Cadmium Morphometry Stereology |
description |
Cadmium (Cd) present in the air, drinking water and food has the potential to affect the health of people, mainly those who live in highly industrialized regions. Cd affects placental function, may cross the placental barrier and directly modify fetal development. It is also excreted into milk. The body is particularly susceptible to Cd exposure during perinatal period. The effect on rat oral epithelium (floor of the mouth) after continuous exposure to drinking water containing low levels of Cd during lactation was studied. Female rats were supplied with ad libitum drinking water containing 300 mg/l of CdCl2 throughout the whole lactation period. Control animals received a similar volume of water without Cd. Lactating rats (21 day-old) were killed by lethal dose of anesthetic. The heads were retrieved, fixed in "alfac" solution (alcohol, acetic acid and formaldehyde) for 24 h, serially sectioned in frontal plane, at the level of the first molars. The 6 µm sections were then stained with hematoxylin and eosin. Nuclear epithelium parameters were estimated, as well as cytoplasm and cell volume, nucleus/cytoplasm ratio, numeric and surface densities, and epithelial thickness. Mean body weight was 34.86 g for the control group and 18.56 g for the Cd-treated group. Histologically, the floor of the mouth epithelium was thinner in the treated group, with smaller and more numerous cells. In this experiment, Cd induced epithelial hypotrophy, indicating a direct action in oral mucosa cells, besides retarded development of the pups. |
publishDate |
2004 |
dc.date.none.fl_str_mv |
2004-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242004000200003 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242004000200003 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1806-83242004000200003 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Pesquisa Odontológica - SBPqO |
publisher.none.fl_str_mv |
Sociedade Brasileira de Pesquisa Odontológica - SBPqO |
dc.source.none.fl_str_mv |
Brazilian Oral Research v.18 n.2 2004 reponame:Brazilian Oral Research instname:Sociedade Brasileira de Pesquisa Odontológica (SBPqO) instacron:SBPQO |
instname_str |
Sociedade Brasileira de Pesquisa Odontológica (SBPqO) |
instacron_str |
SBPQO |
institution |
SBPQO |
reponame_str |
Brazilian Oral Research |
collection |
Brazilian Oral Research |
repository.name.fl_str_mv |
Brazilian Oral Research - Sociedade Brasileira de Pesquisa Odontológica (SBPqO) |
repository.mail.fl_str_mv |
pob@edu.usp.br||bor@sbpqo.org.br |
_version_ |
1750318320283811840 |