Are bovine teeth a suitable substitute for human teeth in in vitro studies to assess endotoxin load in root canals?
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Oral Research |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242015000100282 |
Resumo: | The present study aimed to determine the feasibility of using bovine teeth as a suitable alternative for human teeth, in experiments involving in vitro endotoxin contamination. Twenty bovine central incisors and 20 human single-root premolars had their dental crowns removed and root lengths set at 16 mm. Root canals were prepared up to #60 K-file size and sterilized with cobalt-60 gamma irradiation (20 kGy, 6 h). The teeth were randomly divided into four groups: G1-bovine teeth (bovine negative control, n = 10), G2-human teeth (human negative control, n = 10), G3-bovine teeth, inoculated withEscherichia coli (055:B55) LPS, and G4-human teeth inoculated with E. coli LPS. The G1 and G2 groups were exposed to apyrogenic water. After the teeth had been incubated at 37 °C and atmospheric humidity for 24 h, the samples of solutions in the main canals were collected with apyrogenic absorbent paper tips. LPS levels were quantified using Limulus Amebocyte Lysate assay. The data obtained were statistically analyzed using one-way ANOVA, with a significance level of 5%. A high amount of endotoxin was detected in the inoculated human teeth (G4) when compared to the sterilized teeth (G2), as well as in the inoculated bovine teeth (G3) when compared to the inoculated human teeth (G4). However, there was no statistical difference between bovine teeth before and after the E. coli endotoxin inoculation. Therefore, under the mentioned experimental conditions, the use of bovine teeth should not be a choice for laboratory research on endotoxin contamination. |
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Are bovine teeth a suitable substitute for human teeth in in vitro studies to assess endotoxin load in root canals?Dental Pulp CavityEndotoxinsDecontaminationHumansCattleThe present study aimed to determine the feasibility of using bovine teeth as a suitable alternative for human teeth, in experiments involving in vitro endotoxin contamination. Twenty bovine central incisors and 20 human single-root premolars had their dental crowns removed and root lengths set at 16 mm. Root canals were prepared up to #60 K-file size and sterilized with cobalt-60 gamma irradiation (20 kGy, 6 h). The teeth were randomly divided into four groups: G1-bovine teeth (bovine negative control, n = 10), G2-human teeth (human negative control, n = 10), G3-bovine teeth, inoculated withEscherichia coli (055:B55) LPS, and G4-human teeth inoculated with E. coli LPS. The G1 and G2 groups were exposed to apyrogenic water. After the teeth had been incubated at 37 °C and atmospheric humidity for 24 h, the samples of solutions in the main canals were collected with apyrogenic absorbent paper tips. LPS levels were quantified using Limulus Amebocyte Lysate assay. The data obtained were statistically analyzed using one-way ANOVA, with a significance level of 5%. A high amount of endotoxin was detected in the inoculated human teeth (G4) when compared to the sterilized teeth (G2), as well as in the inoculated bovine teeth (G3) when compared to the inoculated human teeth (G4). However, there was no statistical difference between bovine teeth before and after the E. coli endotoxin inoculation. Therefore, under the mentioned experimental conditions, the use of bovine teeth should not be a choice for laboratory research on endotoxin contamination.Sociedade Brasileira de Pesquisa Odontológica - SBPqO2015-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242015000100282Brazilian Oral Research v.29 n.1 2015reponame:Brazilian Oral Researchinstname:Sociedade Brasileira de Pesquisa Odontológica (SBPqO)instacron:SBPQO10.1590/1807-3107BOR-2015.vol29.0087info:eu-repo/semantics/openAccessMELO,Tiago André Fontoura deGRÜNDLING,Grasiela Sabrina LonghiMONTAGNER,FranciscoSCARPARO,Roberta KochenborgerFIGUEIREDO,José Antônio Poli deVIER-PELISSER,Fabiana Vieiraeng2018-09-17T00:00:00Zoai:scielo:S1806-83242015000100282Revistahttps://www.scielo.br/j/bor/https://old.scielo.br/oai/scielo-oai.phppob@edu.usp.br||bor@sbpqo.org.br1807-31071806-8324opendoar:2018-09-17T00:00Brazilian Oral Research - Sociedade Brasileira de Pesquisa Odontológica (SBPqO)false |
dc.title.none.fl_str_mv |
Are bovine teeth a suitable substitute for human teeth in in vitro studies to assess endotoxin load in root canals? |
title |
Are bovine teeth a suitable substitute for human teeth in in vitro studies to assess endotoxin load in root canals? |
spellingShingle |
Are bovine teeth a suitable substitute for human teeth in in vitro studies to assess endotoxin load in root canals? MELO,Tiago André Fontoura de Dental Pulp Cavity Endotoxins Decontamination Humans Cattle |
title_short |
Are bovine teeth a suitable substitute for human teeth in in vitro studies to assess endotoxin load in root canals? |
title_full |
Are bovine teeth a suitable substitute for human teeth in in vitro studies to assess endotoxin load in root canals? |
title_fullStr |
Are bovine teeth a suitable substitute for human teeth in in vitro studies to assess endotoxin load in root canals? |
title_full_unstemmed |
Are bovine teeth a suitable substitute for human teeth in in vitro studies to assess endotoxin load in root canals? |
title_sort |
Are bovine teeth a suitable substitute for human teeth in in vitro studies to assess endotoxin load in root canals? |
author |
MELO,Tiago André Fontoura de |
author_facet |
MELO,Tiago André Fontoura de GRÜNDLING,Grasiela Sabrina Longhi MONTAGNER,Francisco SCARPARO,Roberta Kochenborger FIGUEIREDO,José Antônio Poli de VIER-PELISSER,Fabiana Vieira |
author_role |
author |
author2 |
GRÜNDLING,Grasiela Sabrina Longhi MONTAGNER,Francisco SCARPARO,Roberta Kochenborger FIGUEIREDO,José Antônio Poli de VIER-PELISSER,Fabiana Vieira |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
MELO,Tiago André Fontoura de GRÜNDLING,Grasiela Sabrina Longhi MONTAGNER,Francisco SCARPARO,Roberta Kochenborger FIGUEIREDO,José Antônio Poli de VIER-PELISSER,Fabiana Vieira |
dc.subject.por.fl_str_mv |
Dental Pulp Cavity Endotoxins Decontamination Humans Cattle |
topic |
Dental Pulp Cavity Endotoxins Decontamination Humans Cattle |
description |
The present study aimed to determine the feasibility of using bovine teeth as a suitable alternative for human teeth, in experiments involving in vitro endotoxin contamination. Twenty bovine central incisors and 20 human single-root premolars had their dental crowns removed and root lengths set at 16 mm. Root canals were prepared up to #60 K-file size and sterilized with cobalt-60 gamma irradiation (20 kGy, 6 h). The teeth were randomly divided into four groups: G1-bovine teeth (bovine negative control, n = 10), G2-human teeth (human negative control, n = 10), G3-bovine teeth, inoculated withEscherichia coli (055:B55) LPS, and G4-human teeth inoculated with E. coli LPS. The G1 and G2 groups were exposed to apyrogenic water. After the teeth had been incubated at 37 °C and atmospheric humidity for 24 h, the samples of solutions in the main canals were collected with apyrogenic absorbent paper tips. LPS levels were quantified using Limulus Amebocyte Lysate assay. The data obtained were statistically analyzed using one-way ANOVA, with a significance level of 5%. A high amount of endotoxin was detected in the inoculated human teeth (G4) when compared to the sterilized teeth (G2), as well as in the inoculated bovine teeth (G3) when compared to the inoculated human teeth (G4). However, there was no statistical difference between bovine teeth before and after the E. coli endotoxin inoculation. Therefore, under the mentioned experimental conditions, the use of bovine teeth should not be a choice for laboratory research on endotoxin contamination. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242015000100282 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242015000100282 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1807-3107BOR-2015.vol29.0087 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Pesquisa Odontológica - SBPqO |
publisher.none.fl_str_mv |
Sociedade Brasileira de Pesquisa Odontológica - SBPqO |
dc.source.none.fl_str_mv |
Brazilian Oral Research v.29 n.1 2015 reponame:Brazilian Oral Research instname:Sociedade Brasileira de Pesquisa Odontológica (SBPqO) instacron:SBPQO |
instname_str |
Sociedade Brasileira de Pesquisa Odontológica (SBPqO) |
instacron_str |
SBPQO |
institution |
SBPQO |
reponame_str |
Brazilian Oral Research |
collection |
Brazilian Oral Research |
repository.name.fl_str_mv |
Brazilian Oral Research - Sociedade Brasileira de Pesquisa Odontológica (SBPqO) |
repository.mail.fl_str_mv |
pob@edu.usp.br||bor@sbpqo.org.br |
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1750318324307197952 |