Flow Injection Analysis System for Screening Organophosphorus Pesticides by their Inhibitory Effect on the Enzyme Acethylcholinesterase

Detalhes bibliográficos
Autor(a) principal: Silva,Marcos P.
Data de Publicação: 2015
Outros Autores: Tubino,Matthieu, Elsholz,Tereza C. R., Elsholz,Olaf, Khan,Sabir, Vila,Marta M. D. C.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Journal of the Brazilian Chemical Society (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532015000300484
Resumo: A flow-injection spectrophotometric procedure was developed for screening organophosphorus pesticides. The method is based on the inhibition of acetylcholinesterase immobilized on controlled porous glass beads with acetylcholine chloride as the substrate. Methyl parathion, chlorpyrifos, malathion and dichlorvos have been tested. The analytical peak height for a given acetylcholine chloride concentration correlates linearly with the logarithmic concentration of the pesticides between 1.0 × 10-3 mol L-1 and 1.0 × 10-6 mol L-1. If bromine water is added to the pesticide solution, a dramatic increase is observed in the analytical signal, and correlations with the logarithm of the concentrations are observed from 1.0 × 10-3 to 1.0 × 10-10 mol L-1.
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spelling Flow Injection Analysis System for Screening Organophosphorus Pesticides by their Inhibitory Effect on the Enzyme AcethylcholinesterasepesticidesorganophosphorusacetylcholinesterasebiosensorA flow-injection spectrophotometric procedure was developed for screening organophosphorus pesticides. The method is based on the inhibition of acetylcholinesterase immobilized on controlled porous glass beads with acetylcholine chloride as the substrate. Methyl parathion, chlorpyrifos, malathion and dichlorvos have been tested. The analytical peak height for a given acetylcholine chloride concentration correlates linearly with the logarithmic concentration of the pesticides between 1.0 × 10-3 mol L-1 and 1.0 × 10-6 mol L-1. If bromine water is added to the pesticide solution, a dramatic increase is observed in the analytical signal, and correlations with the logarithm of the concentrations are observed from 1.0 × 10-3 to 1.0 × 10-10 mol L-1.Sociedade Brasileira de Química2015-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532015000300484Journal of the Brazilian Chemical Society v.26 n.3 2015reponame:Journal of the Brazilian Chemical Society (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.5935/0103-5053.20150301info:eu-repo/semantics/openAccessSilva,Marcos P.Tubino,MatthieuElsholz,Tereza C. R.Elsholz,OlafKhan,SabirVila,Marta M. D. C.eng2017-11-09T00:00:00Zoai:scielo:S0103-50532015000300484Revistahttp://jbcs.sbq.org.brONGhttps://old.scielo.br/oai/scielo-oai.php||office@jbcs.sbq.org.br1678-47900103-5053opendoar:2017-11-09T00:00Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)false
dc.title.none.fl_str_mv Flow Injection Analysis System for Screening Organophosphorus Pesticides by their Inhibitory Effect on the Enzyme Acethylcholinesterase
title Flow Injection Analysis System for Screening Organophosphorus Pesticides by their Inhibitory Effect on the Enzyme Acethylcholinesterase
spellingShingle Flow Injection Analysis System for Screening Organophosphorus Pesticides by their Inhibitory Effect on the Enzyme Acethylcholinesterase
Silva,Marcos P.
pesticides
organophosphorus
acetylcholinesterase
biosensor
title_short Flow Injection Analysis System for Screening Organophosphorus Pesticides by their Inhibitory Effect on the Enzyme Acethylcholinesterase
title_full Flow Injection Analysis System for Screening Organophosphorus Pesticides by their Inhibitory Effect on the Enzyme Acethylcholinesterase
title_fullStr Flow Injection Analysis System for Screening Organophosphorus Pesticides by their Inhibitory Effect on the Enzyme Acethylcholinesterase
title_full_unstemmed Flow Injection Analysis System for Screening Organophosphorus Pesticides by their Inhibitory Effect on the Enzyme Acethylcholinesterase
title_sort Flow Injection Analysis System for Screening Organophosphorus Pesticides by their Inhibitory Effect on the Enzyme Acethylcholinesterase
author Silva,Marcos P.
author_facet Silva,Marcos P.
Tubino,Matthieu
Elsholz,Tereza C. R.
Elsholz,Olaf
Khan,Sabir
Vila,Marta M. D. C.
author_role author
author2 Tubino,Matthieu
Elsholz,Tereza C. R.
Elsholz,Olaf
Khan,Sabir
Vila,Marta M. D. C.
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Silva,Marcos P.
Tubino,Matthieu
Elsholz,Tereza C. R.
Elsholz,Olaf
Khan,Sabir
Vila,Marta M. D. C.
dc.subject.por.fl_str_mv pesticides
organophosphorus
acetylcholinesterase
biosensor
topic pesticides
organophosphorus
acetylcholinesterase
biosensor
description A flow-injection spectrophotometric procedure was developed for screening organophosphorus pesticides. The method is based on the inhibition of acetylcholinesterase immobilized on controlled porous glass beads with acetylcholine chloride as the substrate. Methyl parathion, chlorpyrifos, malathion and dichlorvos have been tested. The analytical peak height for a given acetylcholine chloride concentration correlates linearly with the logarithmic concentration of the pesticides between 1.0 × 10-3 mol L-1 and 1.0 × 10-6 mol L-1. If bromine water is added to the pesticide solution, a dramatic increase is observed in the analytical signal, and correlations with the logarithm of the concentrations are observed from 1.0 × 10-3 to 1.0 × 10-10 mol L-1.
publishDate 2015
dc.date.none.fl_str_mv 2015-03-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532015000300484
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532015000300484
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.5935/0103-5053.20150301
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Química
publisher.none.fl_str_mv Sociedade Brasileira de Química
dc.source.none.fl_str_mv Journal of the Brazilian Chemical Society v.26 n.3 2015
reponame:Journal of the Brazilian Chemical Society (Online)
instname:Sociedade Brasileira de Química (SBQ)
instacron:SBQ
instname_str Sociedade Brasileira de Química (SBQ)
instacron_str SBQ
institution SBQ
reponame_str Journal of the Brazilian Chemical Society (Online)
collection Journal of the Brazilian Chemical Society (Online)
repository.name.fl_str_mv Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)
repository.mail.fl_str_mv ||office@jbcs.sbq.org.br
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