The violacein biosynthesis monitored by multi-wavelength fluorescence spectroscopy and by the PARAFAC method
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of the Brazilian Chemical Society (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532012001100014 |
Resumo: | Obtaining information about a biosynthetic pathway is a complex and laborious procedure. In this sense, this work presents a new approach for the initial analysis of the biosynthesis of fluorescent natural products using as example the violacein biosynthesis. For this, a culture of Chromobacterium violaceum was grown in a bioreactor from which aliquots were collected every 2 h for subsequent analysis by multi-wavelength fluorescence spectroscopy. The excitation-emission matrices demonstrated the dynamic behavior of the fluorophores signal that are consumed and produced by the bacterium. These signals were resolved by PARAFAC (parallel factor analysis) method totalizing six pure components. Tryptophan and violacein were identified by comparison to spectra available in the literature. The identification of other fluorophores was critical step due to the lack of a database of fluorescent natural products to compare spectra. Finally, this methodology has great potential to achieve a deeper insight into the biosynthesis of natural products. |
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The violacein biosynthesis monitored by multi-wavelength fluorescence spectroscopy and by the PARAFAC methodbiotransformationviolaceinfluorescence spectroscopycurve resolutionObtaining information about a biosynthetic pathway is a complex and laborious procedure. In this sense, this work presents a new approach for the initial analysis of the biosynthesis of fluorescent natural products using as example the violacein biosynthesis. For this, a culture of Chromobacterium violaceum was grown in a bioreactor from which aliquots were collected every 2 h for subsequent analysis by multi-wavelength fluorescence spectroscopy. The excitation-emission matrices demonstrated the dynamic behavior of the fluorophores signal that are consumed and produced by the bacterium. These signals were resolved by PARAFAC (parallel factor analysis) method totalizing six pure components. Tryptophan and violacein were identified by comparison to spectra available in the literature. The identification of other fluorophores was critical step due to the lack of a database of fluorescent natural products to compare spectra. Finally, this methodology has great potential to achieve a deeper insight into the biosynthesis of natural products.Sociedade Brasileira de Química2012-11-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532012001100014Journal of the Brazilian Chemical Society v.23 n.11 2012reponame:Journal of the Brazilian Chemical Society (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.1590/S0103-50532012005000083info:eu-repo/semantics/openAccessDantas,ClecioVolpe,Pedro L. O.Durán,NelsonFerreira,Márcia M. C.eng2013-01-03T00:00:00Zoai:scielo:S0103-50532012001100014Revistahttp://jbcs.sbq.org.brONGhttps://old.scielo.br/oai/scielo-oai.php||office@jbcs.sbq.org.br1678-47900103-5053opendoar:2013-01-03T00:00Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)false |
dc.title.none.fl_str_mv |
The violacein biosynthesis monitored by multi-wavelength fluorescence spectroscopy and by the PARAFAC method |
title |
The violacein biosynthesis monitored by multi-wavelength fluorescence spectroscopy and by the PARAFAC method |
spellingShingle |
The violacein biosynthesis monitored by multi-wavelength fluorescence spectroscopy and by the PARAFAC method Dantas,Clecio biotransformation violacein fluorescence spectroscopy curve resolution |
title_short |
The violacein biosynthesis monitored by multi-wavelength fluorescence spectroscopy and by the PARAFAC method |
title_full |
The violacein biosynthesis monitored by multi-wavelength fluorescence spectroscopy and by the PARAFAC method |
title_fullStr |
The violacein biosynthesis monitored by multi-wavelength fluorescence spectroscopy and by the PARAFAC method |
title_full_unstemmed |
The violacein biosynthesis monitored by multi-wavelength fluorescence spectroscopy and by the PARAFAC method |
title_sort |
The violacein biosynthesis monitored by multi-wavelength fluorescence spectroscopy and by the PARAFAC method |
author |
Dantas,Clecio |
author_facet |
Dantas,Clecio Volpe,Pedro L. O. Durán,Nelson Ferreira,Márcia M. C. |
author_role |
author |
author2 |
Volpe,Pedro L. O. Durán,Nelson Ferreira,Márcia M. C. |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Dantas,Clecio Volpe,Pedro L. O. Durán,Nelson Ferreira,Márcia M. C. |
dc.subject.por.fl_str_mv |
biotransformation violacein fluorescence spectroscopy curve resolution |
topic |
biotransformation violacein fluorescence spectroscopy curve resolution |
description |
Obtaining information about a biosynthetic pathway is a complex and laborious procedure. In this sense, this work presents a new approach for the initial analysis of the biosynthesis of fluorescent natural products using as example the violacein biosynthesis. For this, a culture of Chromobacterium violaceum was grown in a bioreactor from which aliquots were collected every 2 h for subsequent analysis by multi-wavelength fluorescence spectroscopy. The excitation-emission matrices demonstrated the dynamic behavior of the fluorophores signal that are consumed and produced by the bacterium. These signals were resolved by PARAFAC (parallel factor analysis) method totalizing six pure components. Tryptophan and violacein were identified by comparison to spectra available in the literature. The identification of other fluorophores was critical step due to the lack of a database of fluorescent natural products to compare spectra. Finally, this methodology has great potential to achieve a deeper insight into the biosynthesis of natural products. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-11-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532012001100014 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532012001100014 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0103-50532012005000083 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
dc.source.none.fl_str_mv |
Journal of the Brazilian Chemical Society v.23 n.11 2012 reponame:Journal of the Brazilian Chemical Society (Online) instname:Sociedade Brasileira de Química (SBQ) instacron:SBQ |
instname_str |
Sociedade Brasileira de Química (SBQ) |
instacron_str |
SBQ |
institution |
SBQ |
reponame_str |
Journal of the Brazilian Chemical Society (Online) |
collection |
Journal of the Brazilian Chemical Society (Online) |
repository.name.fl_str_mv |
Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ) |
repository.mail.fl_str_mv |
||office@jbcs.sbq.org.br |
_version_ |
1750318174422695936 |